Chromatographic Determination of Denatonium Benzoate in Colognes

Year 2024, , 1840 - 1848, 23.10.2024

Emine Kübra İnal

https://doi.org/10.29130/dubited.1409022

Abstract

Denatonium benzoate, commercially known as Bitrex, is an ionic compound containing a quaternary ammonium cation (denatonium) and negatively charged benzoate. It is commonly used as a bittering agent in various alcohol-based products to deter ingestion. The national regulations regarding the production and domestic-foreign trade of ethyl alcohol and methanol states that ethyl alcohols for domestic use should contain 0.8 g denatonium benzoate per 100 L of absolute alcohol. This leads to the necessity of quantitative analysis of denatonium benzoate in alcoholic products. Cologne samples of different brands were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) coupled with UV/vis detector. Isocratic elution was performed on an ODS column with a mobile phase containing 75% MeOH:25% H2O. Since the proposed method does not require any pre-treatment step, the procedure is simple and rapid. The limit of quantitation was found to be 0.17 mg/L. The linearity of the method was tested in the range 0.26 – 15.75 mg/L with a limit of detection of 0.050 mg/L (signal-to-noise ratio of 3).

Keywords

Denatonium benzoate, Bitrex, Denaturant, Alcohol-based hand sanitizers, Cologne, HPLC

Kolonyalarda Denatonyum Benzoatın Kromatografik Tespiti

Abstract

Ticari olarak Bitrex adıyla bilinen denatonyum benzoat, bir kuaterner amonyum katyonu (denatonyum) ve negatif yüklü benzoat içeren iyonik bir bileşiktir. Genellikle çeşitli alkol bazlı ürünlerde kazara yutmayı önlemek amacıyla kullanılır. Etil alkol ve metanol üretimi ve iç-dış ticaretine ilişkin ulusal düzenlemeler, evsel kullanım için etil alkollerin 100 L mutlak alkol başına 0,8 g denatonyum benzoat içermesi gerektiğini belirtmektedir. Bu durum, alkollü ürünlerdeki denatonyum benzoatın nicel analizinin gerekliliğini ortaya koymaktadır. Farklı markalara ait kolonya numuneleri UV/vis dedektörü ile ters-faz yüksek performanslı sıvı kromatografisi (RP-HPLC) kullanılarak analiz edilmiştir. %75 MeOH:%25 H2O içeren mobil faz ile ODS kolonda izokratik elüsyon gerçekleştirilmiştir. Önerilen yöntem herhangi bir ön işlem adımı gerektirmediğinden, işlem basit ve hızlıdır. Alt tayin sınırı 0,17 mg/L olarak bulunmuştur. Yöntemin doğrusallığı 0,26 – 15,75 mg/L aralığında test edilmiş ve gözlenebilme sınırı 0,050 mg/L olarak bulunmuştur (sinyal-gürültü oranı 3).

Keywords

Denatonyum benzoat, Bitrex, Denatüran, Alkol bazlı el dezenfektanı, Kolonya, HPLC

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