Lactifluus Bertillonii Mantarından Sentezlenen Gümüş Nano Partiküllerinin Karakterizasyonu, Mik Yöntemiyle Antimikrobiyal Aktivitesinin Belirlenmesi Ve Moleküler Modelleme Yöntemi İle MAO-A Inhıbıtor Özelliklerinin İncelenmesi

Year 2024, , 2033 - 2049, 23.10.2024

Yasemin Keşkek Karabulut , Aybek Yiğit , Ayşe Karacalı Tunç , Büşra Merve Sarıtaş , Sedat Kesici , Yusuf Uzun , Cemil Sadullahoğlu

https://doi.org/10.29130/dubited.1445798

Abstract

Bu çalışma, minimum inhibitör konsantrasyon (MIC) yöntemi kullanılarak Lactifluus bertillonii mantarlarından yeşil, çevre dostu bir sentez işlemi kullanılarak üretilen AgNP'lerin antimikrobiyal aktivitesine odaklanmaktadır. Ayrıca mantar ekstraktında bulunan kimyasalların inhibitör özellikleri de belirlenmiştir. Çalışmanın bir parçası olarak SEM, TEM, UV-vis ve FT-IR cihazları kullanılmıştır. Karakterizasyondaki ortalama parçacık boyutu, görüntüleme programı tarafından 10.471 nm olarak belirlendi. Ayrıca MIC yöntemi kullanılarak yapılan antimikrobiyal aktivite testlerinde AgNP'lerin Klebsiella pneumoniae'ye karşı aktivitesinin 512 µg/ml olması daha hassas sonuçlar vermektedir. Depresyon tedavisinde hedef enzim olan ve 2Z5X kodlama yapısı insanlardan türetilen MAO-A enzimi, kenetlenme araştırmasında kullanıldı. Isoquercitrin (-8,2 kcal/mol), Rutin (-9,3 kcal/mol), Fisetin (-8,2 kcal/mol), Chrysin (-9,4 kcal/mol), Quercetin (-10,6 kcal/mol), Naringenin (8,8 kcal/mol), Kaemferol (-10,8 kcal/mol) ve Luteolin (-10,8 kcal/mol) üç boyutlu yapıları, DFT/B3LYP/6-31G(d,p) temel seti kullanılarak Gaussian09 programında optimize edildi. Daha sonra AutoDock Vina yazılımı yardımıyla bu yapıların bağlanma enerjileri belirlendi. Bağlanma enerjilerinin MAO-A inhibitörleri özelliğine sahip olduklarını gösterdiği gösterilmiştir.

Keywords

AgNP, Yeşil sentez, Docking, Lactifluus Bertillonii, MAO-A enzimi

MAO-A Inhibitor Properties by Molecular Modeling Method, Antimicrobial Activity and Characterization of Silver Nanoparticles Synthesized from Lactifluus Bertillonii Mushroom

Abstract

This work focuses on the antimicrobial activity of AgNPs produced using a green, environmentally friendly synthesis process from Lactifluus bertillonii mushrooms using the minimum inhibitory concentration (MIC) method. Additionally, the inhibitory characteristics of the chemicals present in the mushroom extract are also determined. SEM, TEM, UV-vis, and FT-IR instruments are employed as part of the study. The average particle size in the characterisation was determined by the imaging program to be 10.471 nm. Additionally, the activity of AgNPs against Klebsiella pneumoniae was found to be 512 µg/ml in the antimicrobial activity tests carried out using the MIC method, which yields more sensitive results. The target enzyme for treating depression, the MAO-A enzyme, whose 2Z5X coding structure was derived from humans, was employed in docking research. The three dimensional structures of Isoquercitrin (-8.2 kcal/mol), Rutin (-9.3 kcal/mol), Fisetin (-8.2 kcal/mol), Chrysin (-9.4 kcal/mol), Quercetin (-10.6 kcal/mol), Naringenin (8.8 kcal/mol), Kaemferol (-10.8 kcal/mol) and Luteolin (-10.8 kcal/mol) were optimized in the Gaussian09 program using the DFT/B3LYP/6-31G(d,p) basis set. Then, binding energies of these structures were determined with the help of the AutoDock Vina software. Their binding energies have been shown to indicate that they possess the property of MAO-A inhibitors.

Keywords

AgNPs, Green synthesis, Docking, Lactifluus bertillonii, MAO-A enzyme

Supporting Institution

Iğdır University

Thanks

The study was conducted at Iğdır University Research Laboratory Application and Research Center (ALUM). We would like to thank you very much for the device and laboratory support you provided us throughout the study.

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