Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes

Uarda Neziri; Aydın Alemdar; Meryem Yıldırım; Cenk Denktaş

doi:10.29130/dubited.1818963

TR EN

Helichrysum arenarium'un PVA/İpek Proteini Bazlı İskele Filmleri Üzerindeki Etkisi: Mekanik, Termal ve Faz Değişimlerinin Ayrıntılı İncelenmesi

Abstract

Bitki özleriyle zenginleştirilmiş biyolojik olarak parçalanabilir iskele sistemleri, çok çeşitli biyomedikal uygulamalarda kritik bir rol oynamaktadır. Bu çalışmada, Helichrysum arenarium özütünün poli(vinil alkol)/ipek proteini (PVA/SP) biyolojik olarak parçalanabilir filmler üzerindeki etkisi ATR/FTIR, XRD, DSC, şişme, mekanik ve MTT analizleriyle karakterize edilmiştir. FTIR analizleri, bitki özütünün iskele yapısına kimyasal olarak entegre olduğunu doğrulamıştır. 3700-2900 cm-1 arasındaki geniş pik (O–H ve N–H gerilme bantları) kaybolmuş ve amid piklerinin yoğunluğu artmıştır. XRD sonuçları, iskelelerin kristal yapısının bozulduğunu göstermiştir. DSC ölçümleri, bitki özütlü iskelelerin erime sıcaklığında (Tm) bir düşüş göstermiştir. Şişme testleri, iskelelerin su tutma kapasitesinin en yüksek özüt oranında (%1,00 ağırlıkça) arttığını ortaya koymuştur. Mekanik analizler, kopma anındaki çekme dayanımının (%20) ve kopma anındaki uzamanın (%34,63) %1,0 ağırlık oranında bitki özütü içeriğiyle azaldığını ortaya koymuştur. MTT analizleri, bitki özütü katkılı iskelelerin fibroblast hücreleriyle biyouyumlu olmadığını göstermiştir. Özütle zenginleştirilmiş biyofilmlerin özelliklerini analiz etmek için yürütülen bu araştırma, özütün belirli dozlarda toksisitesini doğrulamıştır. En biyouyumlu sonuçları elde etmek için, sınırlı dozda H. arenarium özütü kullanılması düşünülmelidir.

Keywords

Altın otu özü, ipek protein, pva, MTT, Termal ve mekanik özellikler

Thanks

Bitkisel özlerle zenginleştirilmiş biyolojik olarak parçalanabilir iskele çalışmalarımızın gerçekleştirilmesinde önemli bir rol oynayan yüksek kaliteli H. arenarium özütünü cömertçe sağlayan MG International Fragrance Company'ye (Gebze, Türkiye) içtenlikle teşekkür ederiz. Ayrıca, bu çalışmada kullanılan ticari ipek proteini (SP) malzemesini cömertçe sağlayan FEBS Biotechnology'ye de teşekkür ederiz.

Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes

Abstract

Biodegradable scaffold systems enhanced with plant extracts play a critical role in a wide range of biomedical applications. In this study, the effect of Helichrysum arenarium extract on poly(vinyl alcohol)/silk protein (PVA/SP) biodegradable films was characterized by ATR/FTIR, XRD, DSC, swelling, mechanical, and MTT analyses. FTIR analyses confirmed the chemical integration of the plant extract into the scaffold structure. The broad peak between 3700-2900 cm-1 (O–H and N–H stretching bands) disappeared, and the intensity of amide peaks increased. XRD results showed that the scaffolds' crystal structure was disrupted. DSC measurements showed a decrease in the melting temperature (Tm) of the plant extract scaffolds. Swelling tests revealed that the water retention capacity of the scaffolds increased at the highest extract ratio (1.00 wt.%). Mechanical analyses revealed that tensile strength at break (20%) and elongation at break (34.63%) decreased with increasing plant extract content. MTT analyses indicated that the plant extract-doped scaffolds were not biocompatible with fibroblast cells at high concentrations, unlike those at lower concentrations. This research, conducted to analyze the properties of biofilms enriched with the extract, confirmed the extract's toxicity at certain doses. To achieve the most biocompatible results, the limited dose of H. arenarium extract should be considered.

Keywords

Helichrysum arenarium, Silk fibroin, PVA, MTT, Thermal and mechanical properties

Supporting Institution

H. arenarium extract was provided by MG International Fragrance Company, and Tannic Acid (TA) was obtained from Balmumcu Kimya.

Ethical Statement

This study does not involve human or animal participants. All procedures followed scientific and ethical principles, and all referenced studies are appropriately cited.

Thanks

We sincerely thank MG International Fragrance Company (Gebze, Türkiye) for generously providing the high-quality H. arenarium extract, which played a crucial role in the implementation of our biodegradable scaffold studies enriched with herbal extracts. We also thank FEBS Biotechnology for generously providing the commercial silk protein (SP) material used in this study.

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Details

Primary Language

English

Subjects

Classical Physics (Other)

Journal Section

Research Article

Authors

Uarda Neziri
0009-0000-3072-442X
Türkiye

Aydın Alemdar
0000-0002-8069-1248
Türkiye

Meryem Yıldırım
0000-0003-1168-2679
Türkiye

Cenk Denktaş ^*
0000-0001-9255-4374
Türkiye

Publication Date

July 24, 2026

Submission Date

November 27, 2025

Acceptance Date

May 21, 2026

Published in Issue

Year 2026 Volume: 14 Number: 3

DOI

https://doi.org/10.29130/dubited.1818963

IZ

https://izlik.org/JA45AA95LS

APA

Neziri, U., Alemdar, A., Yıldırım, M., & Denktaş, C. (2026). Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes. Duzce University Journal of Science and Technology, 14(3), 738-749. https://doi.org/10.29130/dubited.1818963

AMA

1.Neziri U, Alemdar A, Yıldırım M, Denktaş C. Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes. DUBİTED. 2026;14(3):738-749. doi:10.29130/dubited.1818963

Chicago

Neziri, Uarda, Aydın Alemdar, Meryem Yıldırım, and Cenk Denktaş. 2026. “Effect of Helichrysum Arenarium on PVA Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes”. Duzce University Journal of Science and Technology 14 (3): 738-49. https://doi.org/10.29130/dubited.1818963.

EndNote

Neziri U, Alemdar A, Yıldırım M, Denktaş C (July 1, 2026) Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes. Duzce University Journal of Science and Technology 14 3 738–749.

IEEE

[1]U. Neziri, A. Alemdar, M. Yıldırım, and C. Denktaş, “Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes”, DUBİTED, vol. 14, no. 3, pp. 738–749, July 2026, doi: 10.29130/dubited.1818963.

ISNAD

Neziri, Uarda - Alemdar, Aydın - Yıldırım, Meryem - Denktaş, Cenk. “Effect of Helichrysum Arenarium on PVA Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes”. Duzce University Journal of Science and Technology 14/3 (July 1, 2026): 738-749. https://doi.org/10.29130/dubited.1818963.

JAMA

1.Neziri U, Alemdar A, Yıldırım M, Denktaş C. Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes. DUBİTED. 2026;14:738–749.

MLA

Neziri, Uarda, et al. “Effect of Helichrysum Arenarium on PVA Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes”. Duzce University Journal of Science and Technology, vol. 14, no. 3, July 2026, pp. 738-49, doi:10.29130/dubited.1818963.

Vancouver

1.Uarda Neziri, Aydın Alemdar, Meryem Yıldırım, Cenk Denktaş. Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes. DUBİTED. 2026 Jul. 1;14(3):738-49. doi:10.29130/dubited.1818963

Helichrysum arenarium'un PVA/İpek Proteini Bazlı İskele Filmleri Üzerindeki Etkisi: Mekanik, Termal ve Faz Değişimlerinin Ayrıntılı İncelenmesi

Abstract

Keywords

Thanks

Effect of Helichrysum Arenarium on PVA/Silk Protein-Based Scaffold Films: Detailed Investigation of Mechanical, Thermal, and Phase Changes

Abstract

Keywords

Supporting Institution

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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