Anzibel Pastillerde Enoksolon-Benzokain-Klorheksidin HPLC Miktar Tayini

Year 2018, , 1200 - 1210, 01.08.2018

Cumhur Ökçelik Ece Ergun Ümit Ergun

https://doi.org/10.29130/dubited.426743

Abstract

Antiseptik etkili klorheksidini, antienflamatuar etkili enoksolonu ve
lokal etkili benzokaini bir arada içeren ve bu içeriğiyle ağız-boğaz
bölgesindeki lokal irritasyonlara bağlı rahatsızlıkları ortadan kaldırmaya
yardımcı olarak kullanılan Anzibel pastillerin maliyet,
iş yükü ve zaman açısından daha ekonomik olması nedeniyle miktar tayini
analizinin tek bir HPLC yöntemi geliştirilerek, gerekli validasyon çalışmaları
tamamlanmıştır. Analizlerde 25°C'de ACE 5 C18 4.6 x 150 mm, 5mm kolon, 250nm’de
dalga boyunda 0.01 M KH₂PO₄ ‘ın fosforik asit ile pH 3.0±
0.05’e ayarlanmış tampon çözeltisi ve asetonitril kullanılmıştır. Sistem uygunluk,
doğruluk ve doğrusallık parametreleri çalışılmış ve kabul edilebilir sonuçlar
elde edilmiştir.

Keywords

Klorheksidin, enoksolon, benzokain, HPLC

Quantitative HPLC Analysis of Enoxolone-Benzocaine-Chlorhexidine in Anzibel Pastilles

Abstract

In this study, Anzibel pastilles are used in the treatment of the diseases related to the local irritation in oropharyngeal area with the antiseptic effect of a chlorhexidine, antiinflammatory effect of an enoxolone and locally anesthetizing effect of benzocaine. Because of economic reasons such as cost, workload and time, a new simultaneous HPLC method has been developed for the quantitative analysis of anzibel pastilles and the validation studies have been completed. Analysis were performed at 25°C by using ACE 5 C18 4.6 x 150 mm, 5 mm column, mobile phase consisted of a mixture of 0.01 M KH2PO4/H3PO4 buffer (pH = 3.0± 0.05) and acetonitrile and PDA dedector set at 250 nm. System suitability,accuracy and linearity parameters were studied and acceptable results were obtained.

Keywords

Chlorhexidine, Benzocaine, Enoxolone, HPLC

References

• [1] 1RxMediaPharma 2017, İnteraktif İlaç Bilgi Kaynağı
• [2] T. Personen, J. Holmalahti and J. Pohjola, “Determination of chlorhexidine in saliva using high-performance liquid chromatography,” Journal of Chromatography & Biomedical Applications, c. 665, ss. 222-225, 1995.
• [3] L. Havlíková, L. Matysová, L. Nováková, R. Hájková and P. Solich, “HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment,” Journal of Pharmaceutical and Biomedical Analysis, c. 43, ss. 1169-1173, 2007.
• [4] Y. Ha and A. P. Cheung, “New stability-indicating high performance liquid chromatography assay and proposed hydrolytic pathways of chlorhexidine,” Journal of Pharmaceutical and Biomedical Analysis, c. 14, ss. 1327-1334, 1996.
• [5] F. Fiorentino , M. Corrêa, H. Regina and N. Salgado, “Analytical Methods for the Determination of Chlorhexidine: A Review,” Critical Reviews in Analytical Chemistry, c. 40, s. 2, ss. 89-101, 2010
• [6] L.R. Paschoal and W.A. Ferreira, "Simultaneous determination of benzocaine and cetylpiridinium chloride in tablets by first-derivative spectrophotometric method," Il Farmaco, c. 55, ss. 687-693, 2000.
• [7] P. Perez-Lozano, E. Garcia-Montoya, A. Orriols, M. Minarro, J.R. Tico and J.M. Sune-Negre, "A new validated method for the simultaneous determination of benzocaine, propylparaben and benzyl alcohol in a bioadhesive gel by HPLC," Journal of Pharmaceutical and Biomedical Analysis, c. 39, ss. 920-927, 2005.
• [8] H. A. Merey, "Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine," Bulletin of Faculty of Pharmacy, c. 54, ss. 181-189, 2016.
• [9] H. A. Merey and H. E. Zaazaa, "Validated simultaneous determination of antipyrine and benzocaine HCl in the presence of benzocaine HCl degradation product," Royal Society of Chemistry: Analytical Methods, c. 6, ss. 6044-6050, 2014.
• [10] S. Esmaeili, F. Naghibi, M. Mosaddegh and N. Nader, "Determination of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC," Iranian Journal of Pharmaceutical Research, c. 2, ss. 137-141, 2006.
• [11] V. Andrisano, V. Cavrini, D. Bonazzi, "HPLC Determination of 18 [3-Glycyrrhetinic and Glycyrrhizinic Acids in Toothpastes after Solid Phase Extraction," Chromatographia, c. 35, ss. 167-172, 1993.