Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1

Elif Guduk; Gulhan Yasar; Unzile Guven Gulhan; Fatih Aktaş

doi:10.29130/dubited.537340

EN TR

Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1

Abstract

Proteases are hydrolytic enzymes that slice peptide bonds between amino acid residues and these enzymes have various industrial applications including detergent, food, pharmaceutical, leather and diagnostic reagent industries. Among them, alkaline proteases, the most commercialized enzymes in the industry, are of particular interest due to their potential applications in the detergent industry as cleaning additives. In this study, a novel alkaline protease from Bacillus sp. strain EL-GU1 was reported showing highest activity at pH 6 and 25°C. The novel protease was purified by using ammonium sulfate precipitation and identified by 16S rDNA sequencing. Highest activity was observed as 3300 µmol/min^-1mg^-1when casein used as a substrate. Kinetic parameters of the enzyme were determined; K_M, V_max, k_cat and catalytic efficiency values were calculated as 1.4 mM, 1 mM/s, 2.10^-7 s^-1, 0.14 10^-7 s^-1M^-1, respectively. These results indicated that the novel cold active protease from Bacillus sp. strain EL- GU1 can be a good candidate for the detergent industry

Keywords

Bacillus sp.,protease,enzyme characterization,cold active and alkali protease

References

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Elif Guduk
0000-0002-9724-0566
United States

Gulhan Yasar This is me
0000-0001-9759-7410
Türkiye

Unzile Guven Gulhan This is me
0000-0002-5608-0447
Türkiye

Fatih Aktaş ^*
0000-0002-2031-298X
Türkiye

Publication Date

July 31, 2019

Submission Date

March 11, 2019

Acceptance Date

July 17, 2019

Published in Issue

Year 2019 Volume: 7 Number: 3

DOI

https://doi.org/10.29130/dubited.537340

IZ

https://izlik.org/JA96WP83XE

APA

Guduk, E., Yasar, G., Guven Gulhan, U., & Aktaş, F. (2019). Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. Duzce University Journal of Science and Technology, 7(3), 2057-2073. https://doi.org/10.29130/dubited.537340

AMA

1.Guduk E, Yasar G, Guven Gulhan U, Aktaş F. Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. DUBİTED. 2019;7(3):2057-2073. doi:10.29130/dubited.537340

Chicago

Guduk, Elif, Gulhan Yasar, Unzile Guven Gulhan, and Fatih Aktaş. 2019. “Isolation, Purification and Characterization of New Cold Active Subtilisin-Like Protease from Bacillus Sp. Strain EL-GU1”. Duzce University Journal of Science and Technology 7 (3): 2057-73. https://doi.org/10.29130/dubited.537340.

EndNote

Guduk E, Yasar G, Guven Gulhan U, Aktaş F (July 1, 2019) Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. Duzce University Journal of Science and Technology 7 3 2057–2073.

IEEE

[1]E. Guduk, G. Yasar, U. Guven Gulhan, and F. Aktaş, “Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1”, DUBİTED, vol. 7, no. 3, pp. 2057–2073, July 2019, doi: 10.29130/dubited.537340.

ISNAD

Guduk, Elif - Yasar, Gulhan - Guven Gulhan, Unzile - Aktaş, Fatih. “Isolation, Purification and Characterization of New Cold Active Subtilisin-Like Protease from Bacillus Sp. Strain EL-GU1”. Duzce University Journal of Science and Technology 7/3 (July 1, 2019): 2057-2073. https://doi.org/10.29130/dubited.537340.

JAMA

1.Guduk E, Yasar G, Guven Gulhan U, Aktaş F. Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. DUBİTED. 2019;7:2057–2073.

MLA

Guduk, Elif, et al. “Isolation, Purification and Characterization of New Cold Active Subtilisin-Like Protease from Bacillus Sp. Strain EL-GU1”. Duzce University Journal of Science and Technology, vol. 7, no. 3, July 2019, pp. 2057-73, doi:10.29130/dubited.537340.

Vancouver

1.Elif Guduk, Gulhan Yasar, Unzile Guven Gulhan, Fatih Aktaş. Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. DUBİTED. 2019 Jul. 1;7(3):2057-73. doi:10.29130/dubited.537340

Cited By

Cold‐active microbial enzymes and their biotechnological applications

Microbial Biotechnology

https://doi.org/10.1111/1751-7915.14467

Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1

Abstract

Keywords

Soğuk Aktif ve Alkali Subtilisin Benzeri Proteaz Enziminin Bacillus sp. strain EL-GU1’ den İzolasyonu, Saflaştırılması ve Karekterizasyonu

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Cold‐active microbial enzymes and their biotechnological applications