Quantitative HPLC Analysis of Vitamin K3-Amprolium HCl-Methylparaben-Propylparaben in Oral Solutions

Year 2019, , 1224 - 1236, 31.07.2019

Akın Osanmaz Esen Bayraktar Ümit Ergun

https://doi.org/10.29130/dubited.540905

Abstract

HPLC ile Enjeksiyon Çözeltilerde Vitamin K3-Amprolyum HCl-Metilparaben-Propilparaben Miktar Tayini

Abstract

Bu
çalışmada antiprotozoal etkili amprolyum HCl, kanın
pıhtılaşmasını önleme açısından etkili vitamin K3, antimikrobiyal koruyucu
etkili metilparaben ve propilparabeni bir arada içeren oral enjeksiyon
çözeltisinin zaman açısından avantajı nedeniyle tek bir HPLC yöntemi ile miktar
tayini ve validasyon çalışmaları yapılmıştır. Analizlerde
25°C'de İnertsil C8-3 4,6 x 250 mm, 5 µm kolon, 254nm’de dalga boyunda heptan-1-sülfonik
asit sodyum tuzu, glasiyel asetik asit, trietilamin tampon çözeltisi ve asetonitril
ve metanol kullanılmıştır. Seçicilik, sistem uygunluk, doğrusallık ve doğruluk
parametreleri çalışılmış ve kabul edilebilir sonuçlar elde edilmiştir.

Keywords

Vitamin K3, Amprolyum HCl, Metilparaben, Propilparaben, HPLC

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