Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü

Year 2019, , 1279 - 1289, 31.07.2019

Kürşad Yapar Kültiğin Çavuşoğlu , Emine Yalçın , Ali Acar , Güray Demirtaş

https://doi.org/10.29130/dubited.546146

Abstract

In Vivo Protective Role of Nigella sativa L. Against Pb(NO3)2 Induced Toxicity

Abstract

In
this study, the protective role of Nigella
sativa L. seed extract (NSSE) against Pb(NO₃)₂
toxicity in albino mice was investigated. For this purpose, the mice were
randomly divided to six groups. In control group, mice were treated with tap
water and in the treatment groups 500 mg kg^-1 bw Pb(NO₃)₂,
300 and 600 mg kg^-1 bw doses of NSSE were exposed to mice. The
effects of all treatments on organism development were investigated by
determining the changes in body, liver and kidney weights of each group.
Genotoxic effects were determined by investigating the chromosomal
abnormalities (CAs) in bone marrow cells, micronucleus (MN) frequency in
erythrocyte and buccal mucosa cells. As a result, it was observed that Pb(NO₃)₂  treatment resulted in a 6.33-fold decrease in
body weight gain and 2.08 and 1.65-fold increase in liver and kidney weights
compared to control group, respectively. In addition, it was determined that
these alterations in weights were positively changed after NSSE treatment. From
MN assays it was observed that MN frequencies of erythrocyte and buccal mucosa
cells in 500 mg kg^-1 bw
Pb(NO₃)₂ treated group were found as
58.17±5.46 and 19.00±2.76 while in 600 mg kg^-1 bw NSSE+Pb(NO₃)₂
treated group, the frequencies were determined as 32.67±3.78 and
6.50±1.87, respectively. A similar protective role was also observed against
CAs formation, and 600 mg kg^-1 NSSE treatment was found to reduce
the chromatid breaks by 44%. As a result, it has been determined that NSSE can
be used as a protective nutrient against the harmful effects of chemicals such
as heavy metals especially Pb.

Keywords

Albino mice, Body weight, Cytotoxicity, Nigella sativa, Organ weight

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