Molecularly Imprinted Polymer Based Biosensor for Choline

Year 2020, , 974 - 982, 31.01.2020

Melahat Sevgül Bakay , Tuğçe Polat Adil Denizli , Feride Şermin Utku

https://doi.org/10.29130/dubited.578392

Abstract

Biosensors
are systems that can perform a quantitative and/or qualitative analysis of
substances in a liquid or gas environment through their biological recognition
sites and transform the acquired data into detectable signals. Biosensors are
able to detect physical changes (i.e. as density, mass concentration, etc.) by
means of recognition sites and correlate them with electrical or optical
quantities (i.e. current, voltage and impedance). In this study, three
molecularly imprinted pencil graphite electrodes (PGE) with differing numbers
of choline recognition sites, at E-1 M, E-3 M and E-5 M concentration, were
used as electrochemical biosensors. An increase in choline receptor
concentration on the electrode surface was expected to correlate with an
increase in PGE surface bound choline and thus lead to electrical changes. The
study was conducted in a three-electrode cell with Ag/AgCl as the reference
electrode, platinum wire as the counter electrode and PGE as the working
electrode. Cyclic voltammetry and electrochemical impedance measurements were
conducted in 10 mM phosphate buffer solution (PBS) containing 5mM K₃[FeCN₆]^-3/-4
redox pair. As expected, as increasing amount of choline was bound to the
complementary recognition sites on choline imprinted PGEs, a correlating change
in current, voltage and impedance on PGEs was observed. The dynamic detection
range for choline expanded as the choline concentration imprinted on the PGE
electrode increased. Using the E-1 M PGE electrode, 72 pM limit of detection,
up to 7.2 nM limit of linearity was attained.

Keywords

Biosensor, electrochemical impedance spectroscopy, choline, molecularly imprinted polymer, PGE electrode

Kolin Tespiti İçin Moleküler Baskılama Tabanlı Biyosensör Geliştirilmesi

Abstract

Biyolojik sensörün
kısaltması olarak kullanılan biyosensörler, maddelerin sıvı ya da gaz ortamda
nicel veya nitel tayinini sahip olduğu biyolojik tanıma bölgeleri sayesinde
yapabilen ve elde ettiği verileri tespit edilebilir sinyallere çeviren
sistemlerdir. Biyosensörler, uygun tanıma bölgeleri aracılığıyla fiziksel
değişiklikleri (yoğunluk, kütle, derişim vb.) tespit edebilmekte ve bunları
elektriksel veya optik büyüklüklerle (akım, gerilim, empedans vb.)
ilişkilendirmektedir. Bu çalışmada, E-1M, E-3M ve E-5M olmak üzere 3 farklı
derişimde moleküler baskılanmış, farklı sayıda kolin tanıma bölgelerine sahip,
kalem grafit elektrotlar (PGE), elektrokimyasal biyosensörler olarak
kullanılmıştır. Elektrot yüzeyindeki kolin reseptörü konsantrasyonundaki
artışın, PGE yüzeyine bağlı kolindeki artışla ilişkili olması ve dolayısıyla
elektriksel değişikliklere yol açması beklenmektedir. Çalışma, üç elektrotlu
hücrede, referans elektrot olarak Ag/AgCl, 
karşı elektrot olarak platin tel ve çalışma elektrotu olarak PGE
kullanılarak gerçekleştirilmiştir. Elektrotların açık hücre potansiyeli,
dönüşümsel voltametri ve elektrokimyasal empedans ölçümleri, 5mM K₃[FeCN₆]^-3/-4
redoks çifti içeren 10 mM fosfat tampon çözeltisi (PBS) içerisinde
alınmıştır. Çözelti içerisindeki kolinin, kolin baskılanmış PGE'ler üzerindeki
tamamlayıcı tanıma alanlarına bağlanmasıyla beklendiği gibi PGE'lerde akım,
voltaj ve empedans değişimleri gözlenmiştir. Baskılanan molekül
konsantrasyonunun artışıyla bağıntılı olarak tespit aralığında da bir artış
gözlenmiştir. Sonuç olarak, E-1M kolin baskılanan PGE, 7.2 nM-72 pM tespit
aralığındaki kolin konsantrasyonunda en yüksek farklılaşmayı göstermiştir.

Keywords

Biyosensör, elektrokimyasal empedans spektroskopisi, kolin, moleküler baskılama, PGE elektrot

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