Allium cepa Kök Ucu Hücrelerinde Sülfonamid-Aldehit Türevinin Sitotoksik ve Genotoksik Etkileri

Abstract

Kimyasalların sürekli üretimi ve çevreye salınmaları sitotoksisitelerini ve genotoksisitelerini belirleme ihtiyacını ortaya çıkarmıştır. Biyolojik aktivite özellikleri geniş bir spektrumda değişen sülfonamid türevleri, tarım, tıp, eczacılık ve diğer birçok alanda sıklıkla kullanılmaktadır. Bu bileşikler, kullanımları ve çeşitlilikleri nedeniyle ekolojik sistemde önemli bir döngüye sahiptir. Bu çalışmada, sülfonamid-aldehid (SA) türevinin potansiyel sitotoksik ve genotoksik etkileri 6.25, 12.5, 25, 50 ve 100 uM konsantrasyonlarında Allium test sistemi kullanılarak araştırılmıştır. A. cepa hücrelerinde, artan SA konsantrasyonları mitotik indeksi inhibe ederek ve nükleer lezyonları indükleyerek sitotoksik etkilere neden oldu. Ek olarak, artan SA konsantrasyonları, en yaygın olanları C-mitoz, yapışkan metafaz ve anafaz köprüsü olan kromozom anomalileri ve mikronükleusu indükleyerek genotoksik etkilere neden oldu. Sonuçlar gösteriyor ki mikronükleus, nükleer lezyonlar ve kromozom anomalilerinde 25 uM’lık konsantrasyon ve mitotik indekste ise 50 uM’lık konsantrasyon EC50'dir (p <0.05).

Keywords

• Allium Testi
• Azo-Boyalar
• Kromozom Kusuru
• Mikronükleus
• Mitotik İndeks

Cytotoxic and Genotoxic Effects of Sulfonamide-Aldehyde Derivative in Allium cepa Root Tip Cells

Abstract

The continuous production and release into the environment of chemicals has revealed the need to determine their cytotoxicity and genotoxicity. Sulfonamide-aldehyde (SA) derivatives, whose biological activity properties vary in a wide spectrum, are frequently used in agriculture, medicine, pharmacy and many other fields. These compounds have an important cycle in the ecological system due to their use and diversity. In the present study, the potential cytotoxic and genotoxic effects of sulfonamide-aldehyde derivative were investigated using Allium test system with the concentrations of 6.25, 12.5, 25, 50, and 100 μM. In A. cepa cells, the increasing concentrations of SA caused cytotoxic effects by inducing nuclear lesions and inhibition of mitotic index. In addition, the increasing concentrations of SA caused genotoxic effects by inducing micronucleus and chromosome aberrations, which the most common ones are C-mitosis, sticky metaphase, and anaphase bridge. The results indicate that the concentration of 25 μM is EC50 in micronucleus, nuclear lesions, and chromosome aberrations; and 50 μM is EC50 in mitotic index (p < 0.05).

Keywords

• Allium Test
• Azo-Dyes
• Chromosome Aberration
• Micronucleus
• Mitotic index

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