Bazı Pestisitlerin Lipaz Enzimi Üzerine Etkisinin In vitro Koşullarda İncelenmesi

Abstract

Tarım alanında daha yüksek verimde ürün elde etmek için kullanılan pestisitlerin in vivo veya in vitro koşullarda hedef olmayan organizmaların enzim aktivitelerini etkilediği bilinmektedirler. Hedef olmayan organizmaya etki eden pestisitlerin sindirim sistemi enzimi olan lipaz enzimini olumsuz şekilde etkileyebileceği öngörülmüştür. Bu nedenle bu çalışmada tarım alanında yaygın kullanıldıkları bilinen diazinon (organofosfat sınıfı) ve karbaril (karbamat sınıfı) pestisitlerinin lipaz enzimi aktivitesi üzerine in vitro etkisi araştırıldı ve bu pestisitlerin lipaz enzimi üzerine etkili dozaj aralığı tespit edildi. 100-2500 ppm konsantrasyon aralığında diazinon ve karbaril ile muamele edien lipaz enziminin inhibe olduğu gözlemlendi. Maksimum Diazinon konsantrasyonunda lipaz enzim aktivitesi %2,05 olarak hesaplandı. Karbaril ise lipazı maksimum konsantrasyonda tamamen inhibe etti. Diazinon ve karbaril ile muamele edilen lipaz enzimi için Michaelis-Menten ve Lineweaver-Burk grafiği çizildi. Karbarilin, diazinon ile karşılaştırıldığında lipaz enzimi (Ki: 460.96 ± 28.25 mM; IC50: 2.5 ± 1.3 uM) için daha güçlü inhibitör etki gösterdiği belirlendi. Diazinon ile muamele edilen lipaz enziminin Ki ve IC50 değeri sırasıyla 481,32 ± 45,18mM ve 3,6 ± 0,9 uM olarak bulundu. Sonuçlar, C. rugosa lipazının in vitro olarak diazinon ve carbryl tarafından inhibe edildiğini gösterdi. C. rugosa lipazının katalitik aktivitesinin karbaril tarafından yarışmalı bir şekilde, ancak diazinon tarafından yarışmasız bir şekilde inhibe edildiği gösterildi.

Anahtar Kelimeler: Lipaz, Diazinon, Karbaril, Pestisit, İnhibisyon kinetiği

Keywords

• Lipaz
• Diazinon
• Karbaril
• İnhibisyon Kinetiği
• Pestisit

Investigation of Some Pesticides’s Effects on Lipase Under In vitro Conditions

Abstract

It is known that the enzyme activities of non-target organisms are influenced in vivo or in vitro by pesticides that play a major role to obtain higher yields in the field of agriculture. It is foreseen that the pesticides used for various purposes may negatively affect lipase, an enzyme of the digestive system, of the non target organisms. Therefore, in this study, in vitro effect of diazinon (organophosphate class) and carbaryl (carbamate class) pesticides, which are known to be widely used in agriculture, was investigated and an effective dosage range of these pesticides on lipase enzyme was determined. It was observed that the lipase enzyme treated with diazinon and carbaryl in the concentration range of 100-2500 ppm was inhibited. Lipase enzyme activity at maximum diazinon concentration was calculated as 2.05%. Carbaryl completely inhibited lipase at maximum concentration The Kinetics conformed to the Michaelis-Menten model and a Lineweaver-Burk graph of the lipase was drawn. Carbaryl showed the strongest inhibitory effect against lipase (Ki: 460.96±28.25 mM; IC50: 2.5±1.3 µM) in comparison to diazinon. In the case of diazinon, Ki and IC50 value were found as 481.32±45.18mM and 3.6±0.9 µM for the lipase, respectively. The results showed that C. rugosa lipase are inhibited by diazinon and carbryl in vitro. It was shown that the catalytic activity of C. rugosa lipase is inhibited competitively by carbaryl but noncompetitively by diazinon.
Keywords: Lipase, Diazinon, Carbaryl, Pesticide, Inhibition kinetics

Keywords

• Lipase
• Diazinon
• Carbaryl
• Pesticide
• Inhibition kinetic

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