Investigation of Some Pesticides’s Effects on Lipase Under In vitro Conditions
Abstract
It is known that the enzyme activities of non-target organisms are influenced in vivo or in vitro by pesticides that play a major role to obtain higher yields in the field of agriculture. It is foreseen that the pesticides used for various purposes may negatively affect lipase, an enzyme of the digestive system, of the non target organisms. Therefore, in this study, in vitro effect of diazinon (organophosphate class) and carbaryl (carbamate class) pesticides, which are known to be widely used in agriculture, was investigated and an effective dosage range of these pesticides on lipase enzyme was determined. It was observed that the lipase enzyme treated with diazinon and carbaryl in the concentration range of 100-2500 ppm was inhibited. Lipase enzyme activity at maximum diazinon concentration was calculated as 2.05%. Carbaryl completely inhibited lipase at maximum concentration The Kinetics conformed to the Michaelis-Menten model and a Lineweaver-Burk graph of the lipase was drawn. Carbaryl showed the strongest inhibitory effect against lipase (Ki: 460.96±28.25 mM; IC50: 2.5±1.3 µM) in comparison to diazinon. In the case of diazinon, Ki and IC50 value were found as 481.32±45.18mM and 3.6±0.9 µM for the lipase, respectively. The results showed that C. rugosa lipase are inhibited by diazinon and carbryl in vitro. It was shown that the catalytic activity of C. rugosa lipase is inhibited competitively by carbaryl but noncompetitively by diazinon.
Keywords: Lipase, Diazinon, Carbaryl, Pesticide, Inhibition kinetics
Keywords: Lipase, Diazinon, Carbaryl, Pesticide, Inhibition kinetics
Keywords
References
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