Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress

Hülya Torun; Engin Eroğlu; Vesile Yalçın; Elmas Usta

doi:10.29130/dubited.847860

TR EN

Kuraklık Stresi Altında Sarı Kantaronun (Hypericum perforatum L.) Fizikokimyasal ve Antioksidan Tepkileri

Abstract

Bu çalışmada, kuraklık stresinin Hypericum perforatum'daki (St. John's Wort) fizyolojik ve biyokimyasal tepkileri üzerine olan etkileri araştırılmıştır. Kuraklık stresine karşı toleransla ilişkili olarak bu tıbbi ve aromatik bitkide yaprak uzunluğu, bağıl su içeriği (RWC), ozmotik potansiyel, klorofil floresan (Fv/Fm), lipid peroksidasyonu (TBARS), hidrojen peroksit (H2O2), prolin içeriği ve antioksidan sistemdeki (süperoksit dismutaz (SOD), katalaz (CAT), peroksidaz (POX), askorbat peroksidaz (APX) ve glutatyon redüktaz (GR) enzim aktiviteleri) değişimler belirlenmiştir. Doksan günlük fidanlar 3 hafta süreyle kuraklığa maruz bırakılmıştır. Kuraklık altında fizyolojik parametrelerden uzunluk, RWC, Fv/Fm ve ozmotik potansiyel azalmıştır. Kuraklık stresi altında H2O2, TBARS ve prolin seviyeleri önemli ölçüde artmıştır; ancak kontrol grupları ile kıyaslandığında bu artış prolin içeriğinde (25.9 kat) daha belirgindir. Yüksek prolin birikimi, yapraktaki RWC'nin kuraklık stresi altında hala %83,8 olarak kalmasının bir sonucu olabilir. Diğer taraftan, SOD, CAT ve GR enzim aktiviteleri artarken, POX ve APX aktiviteleri azalmıştır. Sonuçlar, H. perforatum bitkisinde kuraklık stresine karşı geliştirilmiş toleransın, artan antioksidatif savunma sistemi kapasitesi ile başarılabileceğini göstermektedir.

Keywords

Antioksidan savunma, Kuraklık stresi, Hypericum perforatum, Sarı kantaron

Project Number

2019.02.01.970

Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress

Abstract

This study investigated the effects of drought stress on the physiological and biochemical responses of the medicinal and aromatic plant Hypericum perforatum (St. John’s Wort). Changes were determined in leaf length, relative water content (RWC), osmotic potential, chlorophyll fluorescence (Fv/Fm), lipid peroxidation (TBARS), hydrogen peroxide (H2O2), and proline content as well as in the antioxidant system enzyme activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), and glutathione reductase (GR). These responses were examined in relation to the tolerance of drought stress in H. perforatum. Ninety-day-old seedlings were subjected to drought for three weeks. The physiological parameters of leaf length, RWC, Fv/Fm, and osmotic potential were reduced under drought. The H2O2, TBARS, and proline levels were increased significantly under drought stress. Moreover, the proline content increase was greatly pronounced (25.9-fold) compared to the control groups. The high accumulation of proline may have resulted from the 83.8% leaf RWC still remaining under drought stress. On the other hand, the SOD, CAT, and GR enzyme activities were enhanced, whereas the POX and APX activities were reduced. The results indicate that improved tolerance to drought stress in H. perforatum plants may be accomplished through increased capacity of the antioxidative defense system

Keywords

Antioxidant defense, Drought stress, Hypericum perforatum, St. John’s wort

Supporting Institution

Duzce University

Project Number

2019.02.01.970

Thanks

This work was supported by funding from the Düzce University Research Foundation (Grant number 2019.02.01.970).

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Hülya Torun ^*
0000-0002-1118-5130
Türkiye

Engin Eroğlu
0000-0002-1777-8375
Türkiye

Vesile Yalçın
0000-0003-1293-732X
Türkiye

Elmas Usta This is me
0000-0001-8877-1106
Türkiye

Publication Date

January 31, 2021

Submission Date

December 27, 2020

Acceptance Date

January 6, 2021

Published in Issue

Year 2021 Volume: 9 Number: 1

DOI

https://doi.org/10.29130/dubited.847860

IZ

https://izlik.org/JA95DL89LJ

APA

Torun, H., Eroğlu, E., Yalçın, V., & Usta, E. (2021). Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. Duzce University Journal of Science and Technology, 9(1), 40-50. https://doi.org/10.29130/dubited.847860

AMA

1.Torun H, Eroğlu E, Yalçın V, Usta E. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DUBİTED. 2021;9(1):40-50. doi:10.29130/dubited.847860

Chicago

Torun, Hülya, Engin Eroğlu, Vesile Yalçın, and Elmas Usta. 2021. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Duzce University Journal of Science and Technology 9 (1): 40-50. https://doi.org/10.29130/dubited.847860.

EndNote

Torun H, Eroğlu E, Yalçın V, Usta E (January 1, 2021) Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. Duzce University Journal of Science and Technology 9 1 40–50.

IEEE

[1]H. Torun, E. Eroğlu, V. Yalçın, and E. Usta, “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress”, DUBİTED, vol. 9, no. 1, pp. 40–50, Jan. 2021, doi: 10.29130/dubited.847860.

ISNAD

Torun, Hülya - Eroğlu, Engin - Yalçın, Vesile - Usta, Elmas. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Duzce University Journal of Science and Technology 9/1 (January 1, 2021): 40-50. https://doi.org/10.29130/dubited.847860.

JAMA

1.Torun H, Eroğlu E, Yalçın V, Usta E. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DUBİTED. 2021;9:40–50.

MLA

Torun, Hülya, et al. “Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum Perforatum L.) under Drought Stress”. Duzce University Journal of Science and Technology, vol. 9, no. 1, Jan. 2021, pp. 40-50, doi:10.29130/dubited.847860.

Vancouver

1.Hülya Torun, Engin Eroğlu, Vesile Yalçın, Elmas Usta. Physicochemical and Antioxidant Responses of St. John’s Wort (Hypericum perforatum L.) under Drought Stress. DUBİTED. 2021 Jan. 1;9(1):40-5. doi:10.29130/dubited.847860

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