Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme

Kemal Çetin

doi:10.29130/dubited.891731

EN TR

Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme

Abstract

Hydrogels exhibiting selectivity towards lysozyme were produced by metal-ion assisted-imprinting technology. A metal-chelate monomer N-vinyl-2-pyrrolidone is pre-assembled with the template molecule via assistance of Cu(II) ions and co-polymerized with 2-hydroxyethyl methacrylate. Lysozyme imprinted hydrogels were characterized by Fourier transform infrared spectroscopy, swelling tests, scanning electron microscopy. The conditions for the optimum adsorption capacity of the hydrogels towards lysozyme were found out by investigating the effects of initial concentration of lysozyme, medium pH, contact time and ionic strength. Maximum adsorption of lysozyme on poly(hydroxyethyl methacrylate-co-N-vinyl pyrrolidone) hydrogel was found to be 12.25 mg/g for 1.0 mg/mL initial concentration of lysozyme at 25.0°C with an optimal pH of 7.0. After ten adsorption-desorption cycles with the same hydrogel, the lysozyme adsorption capacity decreased by 13.80%.

Keywords

Hydrogels, lysozyme, metal ion coordination, molecular imprinting

References

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Kemal Çetin ^*
0000-0002-7393-7377
Türkiye

Publication Date

May 29, 2021

Submission Date

March 5, 2021

Acceptance Date

April 8, 2021

Published in Issue

Year 2021 Volume: 9 Number: 3

DOI

https://doi.org/10.29130/dubited.891731

IZ

https://izlik.org/JA65YU26CB

APA

Çetin, K. (2021). Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme. Duzce University Journal of Science and Technology, 9(3), 545-555. https://doi.org/10.29130/dubited.891731

AMA

1.Çetin K. Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme. DUBİTED. 2021;9(3):545-555. doi:10.29130/dubited.891731

Chicago

Çetin, Kemal. 2021. “Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme”. Duzce University Journal of Science and Technology 9 (3): 545-55. https://doi.org/10.29130/dubited.891731.

EndNote

Çetin K (May 1, 2021) Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme. Duzce University Journal of Science and Technology 9 3 545–555.

IEEE

[1]K. Çetin, “Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme”, DUBİTED, vol. 9, no. 3, pp. 545–555, May 2021, doi: 10.29130/dubited.891731.

ISNAD

Çetin, Kemal. “Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme”. Duzce University Journal of Science and Technology 9/3 (May 1, 2021): 545-555. https://doi.org/10.29130/dubited.891731.

JAMA

1.Çetin K. Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme. DUBİTED. 2021;9:545–555.

MLA

Çetin, Kemal. “Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme”. Duzce University Journal of Science and Technology, vol. 9, no. 3, May 2021, pp. 545-5, doi:10.29130/dubited.891731.

Vancouver

1.Kemal Çetin. Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme. DUBİTED. 2021 May 1;9(3):545-5. doi:10.29130/dubited.891731

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Hittite Journal of Science and Engineering

https://doi.org/10.17350/HJSE19030000320

Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme

Abstract

Keywords

Lizozim Tanımada Metal İyon Destekli Baskılanmış Hidrojeller

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Nucleobase-Modified Microgels Synthesized via Microfabrication Technology for DNA Adsorption