Laktozun Enzimatik Hidrolizi için Dolgulu Kolon Reaktör Parametrelerinin Optimizasyonu

Abstract

Çalışmanın amacı, enzim tutuklu dolgulu kolon reaktör ile laktoz hidrolizinin glikoz ve galaktoza dönüşüm oranını arttımak ve sürekli sistemde yüksek dönüşüm sağlayacak bir reaktör tasarlamaktır. Bu amaçla Duolite A 568 reçinesine tutuklu β-galaktosidaz enzimi içeren dolgulu kolon reaktörün dinamik davranışı moment analizi ve uyarı cevap tekniği ile belirlendi. Kinetik davranış ile dinamik tepki arasındaki ilişki Peclet sayısı (Pe) ve Damkohler sayısı (Da) ile belirlendi. Bu çalışma akış dinamiği, kolon geometrisi, enzim immobilizasyon performansı ve enzimin kolondaki davranışı gibi parametreler kullanılarak matematiksel bir modelin geliştirilmesi ile tamamlandı. Bu veriler doğrultusunda hazırlanan enzim tutuklu dolgulu kolon reaktör sisteminde kullanılması uygun olan partikül büyüklüğü, kolon L/D oranı ve akış hızı sırasıyla 390 μm, 10 ve 4 mL/dk olarak belirlenmiştir. Ayrıca enzim tutuklu dolgulu kolon reaktör ile sütteki laktozdan 55 % dönüşüm elde edilmiştir.

Keywords

• Dolgulu Kolon
• β-galactosidase
• Uyarı Cevap Tekniği
• Moment Analizi

Optimization Packed Bed Column Reactor Parameters for Enzymatic Hydrolyzing of Lactose

Abstract

The purpose of the study to increase the conversion ratio of lactose hydrolysis to glucose and galactose via packed bed reactors containing immobilised enzyme and design a reactor that will achieve a high conversion ratio in continuous systems. For this purpose, the dynamic properties of a packed bed reactor containing immobilised β-galactosidase enzyme on Duolite A 568 resin were determined by a stimulus-response technique and moment analysis. The relation between kinetic behaviour and The dynamic response was determined via Peclet number (Pe) and Damkohler number (Da). This study was completed with the development of a mathematical model using parameters such as flow dynamics, column geometry, enzyme immobilization performance and the behaviour of the enzyme in the column. According to the results, the particle size, L/D ratio and flow rate appropriate for this system were determined as 390 m, 10 and 4 mL/min, respectively. Furthermore, 55% conversion of lactose from milk was obtained using the enzyme immobilized packed bed reactor.

Keywords

• Packed bed column
• β-galactosidase
• Stimulus-response technique
• Moment analysis.

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