HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi

Dilşad Özerkan; Ferdane Danışman Kalındemirtaş; Naci Tüzemen

doi:10.29130/dubited.993669

EN TR

Investigation of 5-Fluorouracil-Induced Cell Death in HCT116 and HT29 Colon Cancer Cells by Fourier Transform Infrared Spectroscopy

Abstract

Colon cancer is a common and highly lethal type of cancer all over the world. As with all cancers, monitoring the course of colon cancer is critical. 5-Fluorouracil (5-FU) is an anticancer drug frequently used in colon cancers and causes some biochemical and molecular differences in cells during cell death. In this study, after incubation of HCT116 and HT29 colon cancer cells with 5-FU chemotherapy drug, Fourier transform infrared (FTIR) spectroscopy was used to detect the biochemical change caused by 5FU on cells. The concentration (IC50) of 5-FU at which percent inhibition equals 50 was determined using the MTT method. FTIR measurements were taken after the IC50 value was determined. Accordingly, the IC50 value of 5-FU was found to be 12.69 µg/ml for HCT116 cells and 10.10 µg/ml for HT29 cells, respectively. FTIR spectroscopy showed changes in IR biomarkers such as the increase in lipid content, which is said to be indicators of cell death caused by 5-FU, and the nucleic acid ratio, the α-helix and β-sheet secondary structures of total cellular proteins, and the reduction of amino acid residues, and changes in collagen conformation. As a result, examining cancer cell death with FTIR, which is both a cheap and fast method, can be used as an alternative method.

Keywords

Colon cancer, 5-fluorouracil

HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi

Abstract

Kolon kanseri, tüm dünyada yaygın olan ve yüksek oranda ölümcül bir kanser tipidir. Tüm kanserlerde olduğu gibi kolon kanserinin seyrinin izlenmesi kritik bir öneme sahiptir. 5-Florourasil (5-FU) kolon kanserlerinde sıklıkla kullanılan bir antikanser ilaçtır ve hücre ölümü esnasında hücrelerde bir takım biyokimyasal ve moleküler farklılıklara neden olmaktadır. Bu çalışmada HCT116 ve HT29 kolon kanseri hücreleri 5-FU kemoterapi ilacı ile inkübe edildikten sonra, 5FU’nun hücreler üzerinde oluşturduğu biyokimyasal değişikliğin tespiti için Fourier transform kızılötesi (FTIR) spektroskopisi kullanılmıştır. 5-FU'nun inhibisyon yüzdesinin 50'ye eşit olduğu konsantrasyonu (IC50), MTT yöntemi kullanılarak belirlendi. IC50 değeri saptandıktan sonra FTIR ölçümleri alındı. Buna göre, 5-FU IC50 değeri sırasıyla HCT116 hücreleri için 12,69 µg/ml, HT29 hücreleri için 10,10 µg/ml bulunmuştur. 5-FU’nun sebep olduğu hücre ölümünün göstergeleri olduğu söylenen lipit içeriğindeki artış ile nükleik asit oranı, total hücresel proteinlerin α-sarmal ve β-yaprak ikincil yapıları ve aminoasit kalıntılarının azalması, kollajen konformasyonundaki değişiklikler gibi IR biyobelirteçlerindeki değişiklikler FTIR spektroskopisi ile gösterilmiştir. Sonuç olarak hem ucuz ve hem de hızlı bir yöntem olan FTIR ile kanser hücre ölümünün incelenmesi alternatif bir yöntem olarak kullanılabilir.

Keywords

Kolon kanseri, 5-florourasil, FTIR, Colon cancer, 5-fluorouracil

Thanks

Çalışmada gerek laboratuvar imkanları gerek fikirleriyle bize yol gösteren sayın Prof. Dr. D. Serap KURUCA’ya teşekkürlerimizi iletiriz.

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Details

Primary Language

Turkish

Subjects

Engineering

Journal Section

Research Article

Authors

Dilşad Özerkan ^*
0000-0002-0556-3879
Türkiye

Ferdane Danışman Kalındemirtaş This is me
0000-0001-7085-8596
Türkiye

Naci Tüzemen
0000-0001-8804-5323
Türkiye

Publication Date

January 31, 2023

Submission Date

September 10, 2021

Acceptance Date

May 29, 2022

Published in Issue

Year 2023 Volume: 11 Number: 1

DOI

https://doi.org/10.29130/dubited.993669

IZ

https://izlik.org/JA54YU95WJ

APA

Özerkan, D., Danışman Kalındemirtaş, F., & Tüzemen, N. (2023). HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi. Duzce University Journal of Science and Technology, 11(1), 524-535. https://doi.org/10.29130/dubited.993669

AMA

1.Özerkan D, Danışman Kalındemirtaş F, Tüzemen N. HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi. DUBİTED. 2023;11(1):524-535. doi:10.29130/dubited.993669

Chicago

Özerkan, Dilşad, Ferdane Danışman Kalındemirtaş, and Naci Tüzemen. 2023. “HCT116 Ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi Ile İncelenmesi”. Duzce University Journal of Science and Technology 11 (1): 524-35. https://doi.org/10.29130/dubited.993669.

EndNote

Özerkan D, Danışman Kalındemirtaş F, Tüzemen N (January 1, 2023) HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi. Duzce University Journal of Science and Technology 11 1 524–535.

IEEE

[1]D. Özerkan, F. Danışman Kalındemirtaş, and N. Tüzemen, “HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi”, DUBİTED, vol. 11, no. 1, pp. 524–535, Jan. 2023, doi: 10.29130/dubited.993669.

ISNAD

Özerkan, Dilşad - Danışman Kalındemirtaş, Ferdane - Tüzemen, Naci. “HCT116 Ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi Ile İncelenmesi”. Duzce University Journal of Science and Technology 11/1 (January 1, 2023): 524-535. https://doi.org/10.29130/dubited.993669.

JAMA

1.Özerkan D, Danışman Kalındemirtaş F, Tüzemen N. HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi. DUBİTED. 2023;11:524–535.

MLA

Özerkan, Dilşad, et al. “HCT116 Ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi Ile İncelenmesi”. Duzce University Journal of Science and Technology, vol. 11, no. 1, Jan. 2023, pp. 524-35, doi:10.29130/dubited.993669.

Vancouver

1.Dilşad Özerkan, Ferdane Danışman Kalındemirtaş, Naci Tüzemen. HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi. DUBİTED. 2023 Jan. 1;11(1):524-35. doi:10.29130/dubited.993669

Investigation of 5-Fluorouracil-Induced Cell Death in HCT116 and HT29 Colon Cancer Cells by Fourier Transform Infrared Spectroscopy

Abstract

Keywords

HCT116 ve HT29 Kolon Kanseri Hücrelerinde 5-Florourasil Kaynaklı Hücre Ölümünün Fourier Dönüşümlü Kızılötesi Spektroskopisi ile İncelenmesi

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