Comparison of Antioxidant Activity of Roccella phycopsis Ach. (Roccellaceae) and Flavoparmelia caperata L. Hale (Parmeliaceae) Lichens

Year 2016, Volume: 4 Issue: 3, 837 - 847, 31.07.2016

Sinem Aydın , Kadir Kınalıoğlu

Abstract

The antioxidant activity of Roccella phycopsis Ach. and Flavoparmelia caperata L. Hale lichens was determined by their ability to scavenge free radicals like DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azinobis-(3-ethylbenzothiaziline-6-sulfonate). Total phenolic and flavonoid contents, metal chelating activity and reducing power of the extracs were also measured. Both lichens species displayed noticeable antioxidant activity. The total flavonoid content of methanol and ethanol extracts of the lichens ranged from 110.06±0.004 µg/mL to 154.1±0.007 µg/mL and from 40.96±0.005 µg/mL to 65.23±0.007 µg/mL, respectively. F. caperata lichen showed higher ferric reducing antioxidant power and DPPH radical quenching activity than R. phycopsis lichen. Based on the obtained results, this lichen species are natural sources of antioxidant substances.

Keywords

Roccella phycopsis, Flavoparmelia caperata, Free radical, Antioxidant activity

Roccella phycopsis Ach. (Roccellaceae) ve Flavoparmelia caperata L. Hale (Parmeliaceae) Likenlerinin Antioksidan Aktivitelerinin Karşılaştırılması

Abstract

Roccella phycopsis Ach. ve Flavoparmelia caperata L. Hale likenlerinin antioksidan aktiviteleri DPPH (2,2- difenil-1-pikrilhidrazil) ve ABTS (2,2’-azinobis-(3-etilbenzotiyazilin-6-sulfonat) gibi serbest radikalleri süpürme yetenekleri kullanılarak belirlendi. Ekstraktların toplam fenol ve flavonoid içerikleri, metal şelatlama aktivitesi ve indirgeme gücü de belirlendi. Çalışılan her iki liken ekstraktı da dikkate değer bir antioksidan aktivite sergiledi. Likenlerin metanol ve etanol ekstraktlarının toplam flavonoid içerikleri sırasıyla 110.06±0.004 µg/mL154.1±0.007 µg/mL ile 40.96±0.005 µg/mL 65.23±0.007 µg/mL arasında değişmektedir. F. caperata, R. phycopsis’den daha yüksek demir indirgeyici antioksidan gücü ve DPPH radikali süpürme aktivitesi gösterdi.Bu çalışma, F. caperata ve R. phycopsis likenlerinin etanol ve metanol ekstraktlarının doğal antioksidan kaynağı olarak kullanılabileceğini ortaya koymaktadır.

Keywords

Roccella phycopsis, Flavoparmelia caperata, Serbest radikal, Antioksidan aktivite

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