Bakterilerde Yaşam Stratejisi Olarak Canlı Fakat Kültürü Yapılamayan Durum ve Önemi

Year 2018, Volume: 6 Issue: 1, 108 - 122, 31.01.2018

Cihan Darcan

https://doi.org/10.29130/dubited.347176

Abstract

Bu çalışmada farklılaşma özelliği olmayan (spor
yada kist oluşturamayan) bakterilerin stres koşulları altında yaşamlarını devam
ettirebilmek amacı ile oluşturdukları bir yaşam stratejisi olan dormansi durumu
incelenmiştir. Canlı Fakat Kültürü Yapılamayan Durum (VBNC) hipotezi metabolik
olarak aktif fakat bilinen laboratuar metodları ile kültüre edilemeyen
bakteriyal hücrelerin girmiş olduğu bir bölünememe durumunu tanımlamaktadır. Bu
durum nedeni ile doğal ortamlardan bakterilerin izolasyonları ve sayımlarının
yapılmasında büyük problemler vardır. Ayrıca henüz bütün bakterilere ve bütün
şartlarda uygulanabilecek geleneksel bir metod geliştirilememiştir. İnsan
sağlığı açısından oldukça önemli olan bu durumun çözülmesi gerekmektedir. Fakat
VBNC’nin henüz genetik mekanizması bilinmemektedir.

Keywords

Dormansi, Aktif fakat kültürü yapılamayan(VBNC), Stres

Viable But Non Culturable State as a Survival Strategy in Bacteria and The Important of This State

Abstract

In this study, dormancy state which is a life strategy be able to maintain their lives under stress conditions of the bacteria which are not differentiating feature which can not form a sport or cyst) can be sustained under the stress conditions was investigated. The Viable But Non Culturable State (VBNC) hypothesis is metabolically active but not cultured in the bacterial cells by known laboratory methods. Due to this situation, there is a great problems in isolating and counting bacteria from natural environments. Furthermore, a conventional method that can be applied to both all bacteria and under all conditions has not yet been developed. This situation, which is very important for human health, needs to be solved. The genetic mechanism of VBNC is not yet known.

Keywords

Dormancy, Viable but non culturable (VBNC), Stres

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