Anzibel Pastillerde Enoksolon-Benzokain-Klorheksidin HPLC Miktar Tayini

Year 2018, Volume: 6 Issue: 4, 1200 - 1210, 01.08.2018

Cumhur Ökçelik Ece Ergun Ümit Ergun

https://doi.org/10.29130/dubited.426743

Abstract

Antiseptik etkili klorheksidini, antienflamatuar etkili enoksolonu ve
lokal etkili benzokaini bir arada içeren ve bu içeriğiyle ağız-boğaz
bölgesindeki lokal irritasyonlara bağlı rahatsızlıkları ortadan kaldırmaya
yardımcı olarak kullanılan Anzibel pastillerin maliyet,
iş yükü ve zaman açısından daha ekonomik olması nedeniyle miktar tayini
analizinin tek bir HPLC yöntemi geliştirilerek, gerekli validasyon çalışmaları
tamamlanmıştır. Analizlerde 25°C'de ACE 5 C18 4.6 x 150 mm, 5mm kolon, 250nm’de
dalga boyunda 0.01 M KH₂PO₄ ‘ın fosforik asit ile pH 3.0±
0.05’e ayarlanmış tampon çözeltisi ve asetonitril kullanılmıştır. Sistem uygunluk,
doğruluk ve doğrusallık parametreleri çalışılmış ve kabul edilebilir sonuçlar
elde edilmiştir.

Keywords

Klorheksidin, enoksolon, benzokain, HPLC

Quantitative HPLC Analysis of Enoxolone-Benzocaine-Chlorhexidine in Anzibel Pastilles

Abstract

In this study, Anzibel pastilles are used in the treatment of the diseases related to the local irritation in oropharyngeal area with the antiseptic effect of a chlorhexidine, antiinflammatory effect of an enoxolone and locally anesthetizing effect of benzocaine. Because of economic reasons such as cost, workload and time, a new simultaneous HPLC method has been developed for the quantitative analysis of anzibel pastilles and the validation studies have been completed. Analysis were performed at 25°C by using ACE 5 C18 4.6 x 150 mm, 5 mm column, mobile phase consisted of a mixture of 0.01 M KH2PO4/H3PO4 buffer (pH = 3.0± 0.05) and acetonitrile and PDA dedector set at 250 nm. System suitability,accuracy and linearity parameters were studied and acceptable results were obtained.

Keywords

Chlorhexidine, Benzocaine, Enoxolone, HPLC

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