Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors

Year 2019, Volume: 7 Issue: 3, 1806 - 1812, 31.07.2019

Zehra Banu Bahsi Oral

https://doi.org/10.29130/dubited.558155

Abstract

Alkanethiol
self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed
as a novel probe design in electrochemical biosensors. This paper investigates
the optimization of PNA probe by using two different concentrations of SAMs,
immobilization temperature, and two-stage immobilization process. Alkanethiol
self-assembled monolayers with PNA probe immobilization and cDNA (complementary
DNA) hybridization were measured via AC voltammetry method. The small negative
shift in formal potential (-17 mV) was occurred after cDNA hybridization at
high ionic strength concentration.

Keywords

Alkanethiol self-assembled monolayers, PNA, electrochemical biosensors, AC voltammetry

Elektrokimyasal Biyosensörlerin Geliştirilmesinde Alkanetiol Kendiliğinden Oluşan Tek Katmanlar ile Peptid Nükleik Asit (PNA) Probu

Abstract

Peptid Nükleik Asit (PNA) içeren alkanetiol kendiliğinden
oluşan tek katmanlar (SAM), elektrokimyasal biyosensörlerde prob olarak
önerilmiştir. Bu makale, PNA probunun optimizasyonunu iki farklı SAM konsantrasyonu,
immobilizasyon sıcaklığı ve iki aşamalı immobilizasyon prosesi kullanarak araştırmıştır.
Alkanetiol kendiliğinden oluşan tek katmanlar ile PNA prob immobilizasyonu ve
cDNA (tamamlayıcı DNA) hibridizasyonu AC voltametre metodu ile ölçülmüştür. Yüksek
iyonik kuvvet konsantrasyonunda, cDNA hibridizasyonundan sonra formal
potansiyelde küçük negatif kayma (-17 mV) meydana gelmiştir

Keywords

Alkanetiol kendiliğinden oluşan tek katmanlar, PNA, Elektrokimyasal biyosensörler, AC voltametre

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