The Studies on Antimicrobial Activities of the Lichens Ramalina farinacea (L.) Ach. and Usnea intermedia (A.Massal.) Jatta

Year 2015, Volume: 3 Issue: 2, 340 - 349, 24.04.2015

Neslihan Şirin , Başaran Dülger

Abstract

In this study,Ramalina farinacea (L.) Ach. and Usnea intermedia (A Massal.) Jatta lichens which were collected from The National Park around Bursa-Uludağ were investigated antimicrobial activity against the bacterial cultures; Bacillus cereus ATCC 7064, Bacillus licheniformis ATCC 14580, Citrobacter freundii ATCC 8090, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25992, Klebsiella oxytoca ATCC 8724, Listeria innocua ATCC 33090, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Salmonella enteridis ATCC 13076, Salmonella typhimurium CCM 5445, Shigella flexneri ATCC 12022, Staphylococcus aureus ATCC 6538P, Staphylococcus epidermidis ATCC 3699, Streptococcus pyogenes ATCC 19615, Yersinia pestis ATCC 19428 and the yeast cultures; Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida lipolytica ATCC 8660, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 4563, Cryptococcus neoformans ATCC32045, Debaryomyces hansenii DSM 70238, Saccharomyces cerevisiae ATCC 9796.According to the findings; while the highest antimicrobial activity of Ramalina farinacea (L.) Ach. was found against Salmonella enteritidis ATCC 13076 and Candida albicans ATCC 90028, the highest antimicrobial activity of Usnea intermedia (A.Massal) Jatta was evaluated against Streptococcus pyogenes ATCC 19615 and Candida albicans ATCC 90028. Lichen species were determined to show at different levels of antimicrobial activity against all other microorganisms

Keywords

Antimicrobial activity, Ramalina farinacea, Usnea intermedia

Ramalina farinacea (L.) Ach. ve Usnea intermedia (A.Massal.) Jatta Likenlerinin Antimikrobiyal Aktiviteleri Üzerine Araştırmalar

Abstract

Bu çalışmada Bursa-Uludağ Milli Park civarından toplanan Ramalina farinacea (L.) Ach. ve Usnea intermedia (A.Massal.) Jatta likenlerinin Bacillus cereus ATCC 7064, Bacillus licheniformis ATCC 14580, Citrobacter freundii ATCC 8090, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25992, Klebsiella oxytoca ATCC 8724, Listeria innocua ATCC 33090, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Salmonella enteridis ATCC 13076, Salmonella typhimurium CCM 5445, Shigella flexneri ATCC 12022, Staphylococcus aureus ATCC 6538P, Staphylococcus epidermidis ATCC 3699, Streptococcus pyogenes ATCC 19615, Yersinia pestis ATCC 19428, Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida lipolytica ATCC 8660, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 4563, Cryptococcus neoformans ATCC 32045, Debaryomyces hansenii DSM 70238, Saccharomyces cerevisiae ATCC 9796 türlerine karşı antimikrobiyal aktiviteleri araştırılmıştır. Bulgulara göre; Ramalina farinacea (L.) Ach. likeninin en yüksek antimikrobiyal aktivitesi Salmonella enteritidis ATCC 13076 ve Candida albicans ATCC 90028 türlerine karşı bulunurken, Usnea intermedia (A.Massal) Jatta likeninin en yüksek antimikrobiyal aktivitesi Streptococcus pyogenes ATCC 19615 ve Candida albicans ATCC 90028 türlerinde ölçülmüştür. Liken türlerinin diğer tüm mikroorganizmalara karşı farklı seviyelerde antimikrobiyal aktivite gösterdikleri saptanmıştır.

Keywords

Antimikrobiyal Aktivite, Ramalina farinacea, Usnea intermedia

References

• S. Özenoğlu, G. Aydoğdu, A. B. Dinçsoy, A. A. Taghidizaj K. Derici, K. Yılmaz, S. Aras, D. Cansaran- Duman, Türk Hijyen ve Deneysel Biyoloji Dergisi, 70 (4) (2013) 215-226.
• A. Dilsizoğlu, Z. Kavuncuoğlu, D. Oba, G. Çobanoğlu, Bilim ve Teknik (2004) 86-89.
• P. Srivastava, D. K. Upreti, T. N. Dhole, A. K. Srivastava, M. T. Nayak, Interdisciplinary Pers pective on Infectious Diseases, 2013 (2013).
• NH. Khan, MSA. Nur-e-Kamal, M. Rahman, Indian J. Res. 87 (1988) 395-397.
• CH. Collins, PM Lyne, JM Grance, Microbiological Methods, 6th edition, Butterworths, (1989).
• CLSI (Clinical and Laboratory Standards Institute), Performance Standards for Antimicrobial Disk Susceptibility Test; Approved Standard, CLSI document M02-A11, 11th edition, Clinical and Laboratory Stantards Institute, (2012).
• M. Gulluce, A. Salan, M. Sokmen, F. Sahin, A. Adiguzel, G. Agar, A. Sokmen, Phytomedicine, 13 (2006) 515-521.
• M. T. Saenz, M. D. Garcia, J. G. Rowe, Fitoterapia, 77 (2006) 156-159.
• T. Tay, A. Özdemir Türk, M. Yılmaz, H. Türk, M. Kıvanç, Z. Naturforsch, 59 (2004) 384-387.
• G. Shrestha, L. L. Clair, Bulletin of California Lichen Society, 20 (1) (2013).
• C. O. Esimone, M. U. Adikwu, Fitoterapia, 70 (1999) 428-431.
• A. Agboke, C. Jackson, M. Adedokun , M. A. Momoh, African Journal of Biotechnology, 10 (12) (2011) 2314-2318.
• A.A. Agboke, C.O., Esimone Journal of Medicinal Plants Research, 5 (4) (2011) 644-648.
• B. C. Behera, N. Verma, A. Sonone, U. Makhija, Biotechnology Letters, 27 (2005) 991-995.
• N. Sultana,A.J. Afolayan, Journal of Asian Natural Products Research, 13 (12) (2011) 1158-1164.
• C. Ritika, A. Jayanthi, Iranian Journal of Basic Medical Sciences, 16 (2013) 882-885.
• P. Balaji, G. N. Hariharan, Research Journal of Botany, 2 (1) (2007) 54-59.