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Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital

Year 2015, Volume: 3 Issue: 2, 367 - 372, 28.05.2015

Bahar Gedik , Görkem Dülger

Abstract

The purpose of this study is todetermine antimicrobial activity of extracts of L. stoechasL. againstfivedifferent pathogenic Candida species.As test microorganisms,  species of Candida (Candida albicans, Candida krusei, Candida tropicalis, Candida guilliermondii and Candida glabrata) which were isolated from the patients appyling to Medical Faculty Hospital of Duzce University were used. Ethyl alcohol, chloroform and ethyl asetate extractions were acquired from the plant via Soxhlet. 25 μl, 50 μl and 75 μl of these extractions were impregnated into sterile discs and the anti-candidal activity spectrums were determined by using the disc diffusion method againts test microorganisms.  As the control group standard antifungal antibiotics (Griseofulvin, Fluconazole, Amphotericin b, Miconazole, Nystatin, Flucytosine, Clotrimazole, Ketoconazole, Itraconazole) were used and the outcomes were compared.As a result, it is found out that the extractions acquired from L. stoechas L. plant have a very effective anti-candidal activity as compared with standard antibiotics.On the basis of our results, anti-candidal activity L. stoechas extracts more effective than standard antifungal antibiotics. So, this situation supports the ethnobotany usage of the plant. In addition to this, the potential extracts of the plant commonly used for alternative medicine may be also used for Candidemia treatment in future.

Keywords

Anti-candidal activity Lavandula stoechas L.

References

  • MRS. Zaidan, R. Noor, A. Badrul, AR. Adlin, A. Norazah, AI. Zakiah Trop. Biomed. 22 (2)(2005) 165-170.
  • AC. Goren, G. Topcu, G. Birsel, M. Birsel, Z. Aydogmus, M. PezzutoJ Z Naturforsch.57 (2002) 797-800.
  • MJ Gamez, J Jimenez,S. Risco, A. ZarzueloPharmazie. 42 (1987) 706-707.
  • ES. Giray, S. Kirici, A. Kaya, M. Turk, O. Sonmez, M. InanTalanta. 74 (2008) 930-935.
  • M. Zuzarte, MJ. Goncalves, C. Cavaleiro, JCanhoto, LV. Silva, MJ. Silva, E. Pinto, L. SalgueiroJ. Med. Microbiol. 60 (2011) 612–618.
  • CM. Collins, PM. Lyne, Microbiological Methods. Butterwords and Co. Ltd., (1987)
  • JJ. Walsh, Invasive fungal infections: problems and challenges for developing new antifungal compounds. In: Sutcliffe JA, Georgapapadakov NH, eds., Merging Targets in Antibacterial and Antifungal Chemotherapy. New York, Chapman and Hall(1992)
  • M. Nucci, TF. Queiroz,MT. Alvarado, IN. Tiraboschi, J. Corte, J. ZuritaPlos One.8(3) (2013)e59373.
  • DA. Said, E. Anaissie, O. Uzun, F. Raad, H. Pinzcowski, S. Clin Infect. Dis.(24) (1997) 1122–1128.
  • B. Ozturk, S. KonyalıogluJ. of AARI.15 (1) (2005)61-72.
  • H. Kirmizibekmez,B. Demirci, E. YeşiladaNat. Prod. Commun.4 (7) (2009) 1001-1006.
  • T. Benabdelkader, A. Zitouni, Y. GuittonChembiodiv.8 (3) (2011) 937-953.
  • E. Kakkalou, Planta. Med.54 (1988) 58-59.
  • G. Pintore, M. Usai, P. Bradesi, C. Juliano, G. Boatto,F. Tomi, M. Chessa, R. Cerri, J. CasanovaFlav. Fragr. J. 17 (2002) 15-19.
  • ML. Balchin,G. RothFlav. Fragr. J. 15 (2000) 391-394.
  • SG. Jonathan, O. FasidiI Afr. J. Biomed. Res.6 (2) (2003) 85-90.

Lavandula stoechas L.’ nin hastaneden izole edilen patojenik Candida türlerine karşı anti-candidal aktivitesi

Year 2015, Volume: 3 Issue: 2, 367 - 372, 28.05.2015

Bahar Gedik , Görkem Dülger

Abstract

Bu çalışmanın amacı, beş patojenik Candida türü üzerindeki Lavandula stoechas L. ekstrelerinin antimikrobiyal aktivtesini belirlemektir. Test mikroorganizmaları olarak, Düzce Üniversitesi Tıp Fakültesine başvuran hastalardan izole edilmiş olan Candida (Candida albicans, Candida krusei, Candida tropicalis, Candida guilliermondii ve Candida glabrata) türleri kullanıldı. Etil alkol, kloroform ve etil asetat ekstreleri, Soxhlet aracılığıyla bitkiden elde edildi. Bu ekstrelerden 25 ul, 50 ul ve 75 ul si steril disklere emdirildi ve anti-candidal aktivite spektrumları test mikroorganizmaları üzerinde disk difuzyon metodu kullanılarak belirlendi. Kontrol grubu olarak, standart antifungal antibiyotikler (Griseofulvin, Fluconazole, Amphotericin B, Miconazole, Nystatin, Flucytosine, Clotrimazole, Ketoconazole, Itraconazole) kullanıldı ve sonuçlar karşılaştırıldı. Sonuç itibariyle, L. stoechas L. bitkisiden elde edilen ekstrelerin standart antibiyotiklerle karşılaştırıldığında oldukça etkili anti-candidal bir aktiviteye sahip olduğu ortaya çıktı. Sonuçlarımıza göre, L. stoechas L. ekstrelerinden elde edilen anti-candidal aktivite standart antifungal antibiyotiklerden çok daha fazladır. Bu yüzden, bu durum bitkinin etnobotanik kullanımını desteklemektedir. Buna ilaveten, alternatif tıpta yaygın olarak kullanılan bitkinin potansiyel ekstreleri gelecekte Candidemia tedavisi için de kullanılabilecektir

Keywords

Anti-candidal activity Lavandula stoechas L

References

  • MRS. Zaidan, R. Noor, A. Badrul, AR. Adlin, A. Norazah, AI. Zakiah Trop. Biomed. 22 (2)(2005) 165-170.
  • AC. Goren, G. Topcu, G. Birsel, M. Birsel, Z. Aydogmus, M. PezzutoJ Z Naturforsch.57 (2002) 797-800.
  • MJ Gamez, J Jimenez,S. Risco, A. ZarzueloPharmazie. 42 (1987) 706-707.
  • ES. Giray, S. Kirici, A. Kaya, M. Turk, O. Sonmez, M. InanTalanta. 74 (2008) 930-935.
  • M. Zuzarte, MJ. Goncalves, C. Cavaleiro, JCanhoto, LV. Silva, MJ. Silva, E. Pinto, L. SalgueiroJ. Med. Microbiol. 60 (2011) 612–618.
  • CM. Collins, PM. Lyne, Microbiological Methods. Butterwords and Co. Ltd., (1987)
  • JJ. Walsh, Invasive fungal infections: problems and challenges for developing new antifungal compounds. In: Sutcliffe JA, Georgapapadakov NH, eds., Merging Targets in Antibacterial and Antifungal Chemotherapy. New York, Chapman and Hall(1992)
  • M. Nucci, TF. Queiroz,MT. Alvarado, IN. Tiraboschi, J. Corte, J. ZuritaPlos One.8(3) (2013)e59373.
  • DA. Said, E. Anaissie, O. Uzun, F. Raad, H. Pinzcowski, S. Clin Infect. Dis.(24) (1997) 1122–1128.
  • B. Ozturk, S. KonyalıogluJ. of AARI.15 (1) (2005)61-72.
  • H. Kirmizibekmez,B. Demirci, E. YeşiladaNat. Prod. Commun.4 (7) (2009) 1001-1006.
  • T. Benabdelkader, A. Zitouni, Y. GuittonChembiodiv.8 (3) (2011) 937-953.
  • E. Kakkalou, Planta. Med.54 (1988) 58-59.
  • G. Pintore, M. Usai, P. Bradesi, C. Juliano, G. Boatto,F. Tomi, M. Chessa, R. Cerri, J. CasanovaFlav. Fragr. J. 17 (2002) 15-19.
  • ML. Balchin,G. RothFlav. Fragr. J. 15 (2000) 391-394.
  • SG. Jonathan, O. FasidiI Afr. J. Biomed. Res.6 (2) (2003) 85-90.
There are 16 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Bahar Gedik

Görkem Dülger This is me

Publication Date May 28, 2015
Published in Issue Year 2015 Volume: 3 Issue: 2

Cite

APA Gedik, B., & Dülger, G. (2015). Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital. Duzce University Journal of Science and Technology, 3(2), 367-372.
AMA Gedik B, Dülger G. Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital. DUBİTED. July 2015;3(2):367-372.
Chicago Gedik, Bahar, and Görkem Dülger. “Anti-Candidal Activity of the Lavandula Stoechas L. Against Pathogenic Candida Species Isolated from the Hospital”. Duzce University Journal of Science and Technology 3, no. 2 (July 2015): 367-72.
EndNote Gedik B, Dülger G (July 1, 2015) Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital. Duzce University Journal of Science and Technology 3 2 367–372.
IEEE B. Gedik and G. Dülger, “Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital”, DUBİTED, vol. 3, no. 2, pp. 367–372, 2015.
ISNAD Gedik, Bahar - Dülger, Görkem. “Anti-Candidal Activity of the Lavandula Stoechas L. Against Pathogenic Candida Species Isolated from the Hospital”. Duzce University Journal of Science and Technology 3/2 (July 2015), 367-372.
JAMA Gedik B, Dülger G. Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital. DUBİTED. 2015;3:367–372.
MLA Gedik, Bahar and Görkem Dülger. “Anti-Candidal Activity of the Lavandula Stoechas L. Against Pathogenic Candida Species Isolated from the Hospital”. Duzce University Journal of Science and Technology, vol. 3, no. 2, 2015, pp. 367-72.
Vancouver Gedik B, Dülger G. Anti-candidal activity of the Lavandula stoechas L. against pathogenic Candida species isolated from the hospital. DUBİTED. 2015;3(2):367-72.