Amin Fonksiyonlu Karbon Nanotüp, Kalay Oksit Nanopartikül ve Diamin Oksidaz Temelli Triptamin Biyosensörü

Year 2020, Volume: 8 Issue: 1, 631 - 641, 31.01.2020

Ceren Kaçar , Berna Dalkıran

https://doi.org/10.29130/dubited.602777

Cited By: 1

Abstract

Bu çalışmada amino
fonksiyonlu çok duvarlı karbon nanotüp (NH₂-MWCNT) ve kalay oksit nanopartikül (SnO₂)
ile modifiye edilmiş perde baskılı karbon elektrotlara (SPCE) dayanan
amperometrik triptamin biyosensörü geliştirildi. Diamin oksidaz (DAO) enzimi NH₂-MWCNT-SnO₂/SPCE yüzeyine
N-etil-N′-(3-dimetilaminopropil) karbodiimit (EDC) ve N-hidroksi süksinimit
(NHS) kullanılarak kovalent
bağlama yöntemi ile immobilize edildi. Hazırlanan elektrot yüzeyi, enzimlerin
yüzeyden uzaklaşmasını engellemek ve girişim etkilerini azaltmak amacıyla son
olarak Nafyon ile kaplandı. Biyosensörün yüzey morfolojisi, elektrokimyasal
özellikleri ve analitik performansı taramalı elektron mikroskobu (SEM), dönüşümlü
voltammetri (CV), elektrokimyasal empedans spektroskopi (EIS) ve
kronoamperometri yöntemleri kullanılarak incelendi. Geliştirilen biyosensör ile
triptamin için elde edilen doğrusal çalışma aralığı, gözlenebilme sınırı ve
duyarlık sırası ile 2,0×10^-6 ‒ 2,5×10^-3 M, 6,0×10^-7 M
ve 6,52 µA mM^-1 olarak bulundu. Hazırlanan biyosensörün tekrar kullanılabilirlik ve tekrar üretilebilirliğinin oldukça iyi olduğu
belirlendi.

Keywords

Biyojenik amin, Triptamin, Biyosensör, Karbon nanotüp, Kalay oksit nanopartikül

Supporting Institution

TÜBİTAK

Project Number

116Z159

Thanks

Bu çalışma TÜBİTAK tarafından desteklenmiştir (Proje no: 116Z159).

Tryptamine Biosensor Based on Amino-Functionalized Multiwalled Carbon Nanotubes, Tin Oxide Nanoparticles and Diamine Oxidase

Abstract

In this study, amperometric tryptamine
biosensor based on amino functionalized multiwalled carbon nanotubes (NH₂-MWCNT)
and tin oxide nanoparticles (SnO₂) modified screen-printed carbon electrode
(SPCE) was developed. Diamine oxidase (DAO) enzyme was covalently immobilized
onto NH₂-MWCNT-SnO₂/SPCE
surface via (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (EDC) ve
N-hydroxysuccinimide (NHS) chemistry. The resulting electrode surface was
finally covered with Nafion in order to prevent enzyme leakage from the surface
and minimize the effect of interferences. The surface morphology,
electrochemical bahaviour and analytical performance of the biosensor was
investigated by scanning electron microscopy (SEM), cyclic voltammetry (CV),
electrochemical impedance spectroscopy (EIS) and chronoamperometry methods.
Linear working range, limit of detection and sensitivity of the developed
biosensor was found to be 2.0×10^-6 ‒ 2.5×10^-3 M, 6.0×10^-7
M ve 6.52 µA mM^-1, respectively. Biosensor also showed high
repeatability and reproducibility.

Keywords

Biogenic amine, Tryptamine, Biosensor, Carbon nanotube

Project Number

116Z159

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