Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae)

Year 2020, Volume: 8 Issue: 4, 2289 - 2295, 29.10.2020

Ayşe Aygün Barış Gülcü

https://doi.org/10.29130/dubited.768800

Abstract

In this study, Bacillus thrungiensis aizawai Bonnefoi & de Barjac, 1963 (Florbac), different isolates of Bacillus thuringiensis (Bt) kurstaki Berliner, 1915 (Biobit, Delfin, Rapax, Rebound) and spinosad (Spintor) were assessed for their efficacy against different instars of Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) under laboratory and field conditions in 2019 in Duzce Province, Turkey. Spinosad was the most effective bioinsecticide, followed by Bt aizawai and Bt kurstaki isolates against various instars of H. cunea. In the laboratory, at 96 hour post-treatment, spinosad caused 98% mortality in 2nd and 3rd instars, and 83% mortality in 4th and 5th instars; Bt aizawai caused 98% mortality in 2nd and 3rd instars and 43% mortality in 4th and 5th instars; and Bt kurstaki isolates caused 95% mortality in 2nd and 3rd instars and 51% mortality in 4th and 5th instars. The results from the field study showed similar trends. The older instars were less susceptible to all the tested bioinsecticides than the younger instars, which may be attributable to the phenomenon known as “maturation immunity”. Bacillus thuringiensis kurstaki has already been recommended for management of this pest. Whereas the data indicated that spinosad and Bt aizawai could also be used as bioinsecticides for control H. cunea larvae.

Keywords

Bacillus thuringiensis, spinosad, Hyphantria cunea

Supporting Institution

Düzce Üniversitesi Fen Bilimleri Enstitüsü

Thanks

We thank to Dr. Salih Tunc KAYA from Duzce University for his assistance in the analyses of data. We also thank to Dr. Derya ULUĞ from Adnan Menderes University, Aydın, Turkey and Dr. Harry K. KAYA from University of California, Davis, CA, USA for comments and advice on an earlier version of the manuscript. and for support of this study. This is a dissertation thesis in Institute of Science in Duzce University

Spinosad ve Farklı Bacillus thuringiensis (Bacillaceae) Suşlarının Hyphantria cunea (Lepidoptera: Erebidae) Larvaları Üzerindeki Etkinliğinin Değerlendirilmesi

Abstract

Bu çalışmada, Bacillus thrungiensis aizawai Bonnefoi & de Barjac, 1963 (Florbac), Bacillus thuringiensis (Bt) kurstaki Berliner, 1915 (Biobit, Delfin, Rapax, Rebound)’nin farklı ticari suşları ve spinosad (Spintor)’ın etkinliği Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae)’nın farklı larva dönemlerine karşı laboratuvar ve doğal koşullarda 2019 yılında Düzce ilinde test edilmiştir. Hyphantria cunea’nın farklı dönem larvalarına karşı en etkili biyoinsektisidin spinosad olduğu tespit edilmiştir. Spinosad’ı sırasıyla Bt aizawai ve Bt kurstaki suşları takip etmiştir. Laboratuvar deneylerinde 96 saat sonunda spinosad H. cunea’nın 2. ve 3. dönem larvalarında %98, 4. ve 5. dönem larvalarında ise %83 ölüm meydana getirmiştir. Bt aizawai 2. ve 3. dönem larvalarının %98’ini, 4. ve 5. dönem larvaların ise %43’ünü, Bt kurstaki suşları 2. ve 3. dönem larvaların ortalama %95’ini, 4. ve 5. dönem larvaların ise ortalama %51’ini öldürmüştür. Alan denemelerinde de spinosad en etkili sonuçları sergilemiştir. Çalışmada daha yaşlı larvaların daha az duyarlı oldukları görülmüştür. Bu durumun “maturation immunity” olarak bilinen olguyla bağlantılı olduğu düşünülmektedir. Bu zararlı ile mücadelede Bacillus thrungiensis (Bt) kurstaki zaten tavsiye edilmektedir. Bu çalışmada ise spinosad ve Bt aizawai etken maddesine sahip biyoinsektisitlerin de H. cunea’ya karşı kullanılabileceği gösterilmiştir.

Keywords

Bacillus thuringiensis, spinosad, Hyphantria cunea

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