Ekzojen Salisilik Asit Uygulamalarının Tuz Stresi Altındaki Hıyar Bitkilerinde Fotosistem II Aktivitesi Üzerindeki Etkileri

Year 2021, Volume: 9 Issue: 1, 418 - 429, 31.01.2021

Sezen Toksoy , Ali Doğru

https://doi.org/10.29130/dubited.746811

Abstract

Tuz stresi (100 mM NaCl) altındaki hıyar (Cucumis sativus L.) genotipinde (Beith Alpha F1) ekzojen salisilik asit uygulamasının fotosistem II aktivitesi üzerindeki etkileri klorofil a fluoresansı tekniği yardımıyla araştırılmıştır. 10 günlük hıyar fidelerine 100 mM tuza karşı 50 µM salisilik asit 5 gün süreyle uygulanmıştır. 5. günün sonunda yapılan klorofil a fluoresansı ölçümleri değerlendirilmiştir. Tuz stresi hıyar yapraklarında fotosistem II’nin hem donör hem de akseptör bölgesindeki elektron hareketlerini inhibe etmiştir. Ayrıca tuz stresinin hıyar bitkisinde aktif reaksiyon merkezi miktarını ve kinonA ile plastokinonun indirgenme yeteneğini azalttığı, indirgenmiş reaksiyon merkezlerinin birikimini ve ısı enerjisi kaybını artırdığı belirlenmiştir. Salisilik asit uygulaması ise hıyar bitkilerinde tuz stresinin fotosistem II’nin donör ve akseptör bölgesindeki elektron hareketleri üzerindeki olumsuz etkisini ortadan kaldırmıştır. Ek olarak salisilik asit uygulaması hıyar yapraklarındaki aktif reaksiyon merkezi miktarını ve kinonA ile plastokinonun indirgenme yeteneğini artırırken, indirgenmiş reaksiyon merkezi miktarını ve ısı enerjisi kaybını azaltmıştır. Sonuç olarak salisilik asidin hıyar yapraklarında tuz toleransını artırdığı ve bu yaklaşımın tarımsal amaçlarla kullanılabileceği söylenebilir.

Keywords

Cucumis sativus L., salisilik asit, FSII aktivitesi

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