Toprak Streptomyces İzolatlarının Cryptococcus neoformans Kültürlerine Karşı Antifungal Aktivitesi

Year 2021, Volume: 9 Issue: 2, 591 - 595, 25.04.2021

Gorkem Dulger , Başaran Dülger

https://doi.org/10.29130/dubited.862551

Abstract

Bu çalışmada, Düzce ili topraklardan elde edilen 128 farklı Streptomyces izolatlarının % 26,5'inin Cryptococcus neoformans ATCC 90112 mayasına karşı antifungal aktivite gösterdiği saptanmıştır. Agar plağında oluşan inhibisyon bölgesinin çapı dikkate alınarak izolatlar dört bölüme ayrılmıştır. Buna göre, bölüm 1 (5-10 mm, hafif aktif); bölüm 2 (11-15 mm, orta derecede aktif), bölüm 3 (16-25 mm, yüksek derecede aktif) ve bölüm 4 (26-35 mm, ultra aktif) olarak belirlenmiştir. Bölüm 4'teki 3 izolatın ise kriptokokkoz hastalığına karşı yeni bir antibiyotik kaynağı olabileceği saptanmıştır.

Keywords

Streptomyces, Antifungal Aktivite, Cryptococcus neoformans

Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans

Abstract

In this study, 26.5% of 128 different isolates of Streptomyces recovered from soils in Duzce province, Turkey showed antifungal activity against Cryptococcus neoformans ATCC 90112. Considering the diameter of the inhibition zone formed on the agar plate, isolates were divided into four sections: section 1 (5-10 mm, slightly-active); section 2 (11-15 mm, moderately-active), section 3 (16-25 mm, highly-active) and section 4 (26-35 mm, ultra-active). It is determined that 3 isolates in section 4 may be a source of novel antibiotic against Cryptococcosis.

Keywords

Streptomyces, Antifungal Activity, Cryptococcus neoformans

References

• [1] G. Gebreselema, M. Feleke, S. Samuel, R. Nagappan, “Isolation and characterization of potential antibiotic producing Actinomycetes from water and sediments of Lake Tana Ethiopia”, Asian Pacific Journal of Tropical Biomedicine, vol. 3, pp. 426-35, 2013.
• [2] J. Berdy, “Bioactive microbial metabolites: a personal view”, The Journal of Antibiotics, vol. 58(1), pp. 1-26, 2005.
• [3] I. Saadoun, F. Al-Momani, “Steptomycetes from Jordan soils active against Agrobacterium tumefaciens”, Actinomycetes, vol. 8, pp. 29-36, 1997.
• [4] A.W. Bauer, W.M. Kirby, J.C. Sherris, M. Turk, “Antibiotic susceptibility testing by a standardized single disk method”, American Journal of Clinical Pathology, vol. 45, pp. 493-496, 1966.
• [5] H.M. Atta, “Production, purification, physico-chemical characteristics and biological activities of antifungal antibiotic produced by Streptomyces antibioticus, AZ-Z710”, American-Eurasian Journal of Scientific Research, vol. 5(1), pp. 39-49, 2010.
• [6] S. Ramesh, N. Mathivanan, “Screening of marine Actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes”, World Journal of Microbiology and Biotechnology, vol. 25, pp. 2103–2111, 2009
• [7] P.R. Jensen, P.G. Williams, D.C. Oh, L. Zeigler, W. Fenical, “Species-specific secondary metabolite production in marine Actinomycetes of the genus Salinispora”, Applied and Environmental Microbiology, vol. 73, pp. 1146–1152, 2007.
• [8] A.A. Denizci, “Ege ve Doğu Karadeniz bölgesi topraklarinden izole edilen aktinomisetlerden antibakteriyal antibiyotiklerin aranması ve üretimi üzerine bir araştırma”, Doctoral Tezi, Ege Üniversitesi, Fen Bilimleri Enstitisü, 1996.
• [9] N. Sahin, A. Ugur, “Investigation of the antimicrobial activity of some Streptomyces isolates”, Turkish Journal of Biology, vol. 27, pp. 79-84, 2003.
• [10] M. Mohseni, H. Norouzi, J. Hamedi, A. Roohi, “Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea”, International Journal of Molecular and Cellular Medicine, vol. 2(2), pp. 65-71, 2013.
• [11] C. Coelho, A.L. Bocca, A. Casadevall, “The tools for virulence of Cryptococcus neoformans”, Advances in Applied Microbiology, vol. 87, pp. 1-41, 2014.
• [12] R. Inci, “Kriptokok infeksiyonları”, Flora, vol. 13(2), pp. 61-71, 2008.