Nikel’in Brokoli (Brassica oleracea L. var. italica) Tohumlarının Çimlenmesi ve Fide Gelişimi Üzerine Etkileri

Year 2021, Volume: 9 Issue: 3 - Additional Issue, 226 - 261, 29.05.2021

Çiğdem Çıngıl Barış , Muammer Ünal

https://doi.org/10.29130/dubited.798455

Cited By: 3

Abstract

Bu araştırmada, brokoli bitkisinin tohum ve fidelerinde değişen konsantrasyonlardaki nikelin etkisi ve birikimi incelenmiştir. Tohumlarda çimlenme yüzdesi, bitkinin kök-gövde uzunlukları, ağırlık miktarları, klorofil, karotenoid, total çözünebilir protein ile MDA içerikleri, POD aktiviteleri ve Ni birikimi belirlenmiştir. 100 μM NiSO4 ün tohumlarda çimlenmeyi inhibe ettiği gözlenmiştir. Ni’nin büyümeyi etkilediği, ağırlık miktarlarında da değişikliklere neden olduğu belirlenmiştir. 9 günlük fidelerin klorofil içerikleri 1 μM NiSO4 de artarken, 30 günlük fidelerde ise 0.1 μM NiSO4 de artış tespit edilmiştir. Fidelerdeki total çözünebilir protein içeriğinde 10 ve 100 μM NiSO4 de azalışlar gözlenirken, POD aktivitesinde artışlar gözlenmiştir. Ayrıca, nikelin özellikle köklerde biriktiği belirlenmiştir. Nikelin brokolinin çimlenme ve büyüme-gelişmesi üzerine olan etkileri ile ilgili olarak, düşük konsantrasyonlardan (0.01, 0.1 ve 1 μM) bitkinin olumlu etkilendiği, yüksek konsantrasyonların ise (10 ve 100 μM) toksik etkilere neden olduğu tespit edilmiştir.
Anahtar Kelimeler: Nikel, Çimlenme, Bitki büyümesi, Brokoli, Brassica oleracea L. var. italica, Metal birikimi

Keywords

Nikel, Çimlenme, Bitki büyümesi, Brassica oleracea L. var. italica, Metal birikimi

Supporting Institution

İstanbul Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2265

Thanks

Bu çalışma İstanbul Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir (Proje no: 2265).

The Effects of Nickel on Seed Germination and Seedling Development in Broccoli (Brassica oleracea L. var. italica)

Abstract

In this research, the effect and accumulation of nickel in varying concentrations in broccoli seeds and seedlings were investigated. Germination rate of seeds, root-stem length of the plant, weight amounts, contents of chlorophyll, carotenoid, total soluble protein and MDA, POD activities and Ni accumulation were determined. It was observed that 100 μM NiSO4 inhibited germination in seeds. It has been determined that Ni affect growth and also cause changes in weight amounts. While the chlorophyll content of 9-day-old seedlings increased in 1 μM NiSO4 was applied, an increase was found in 0.1 μM NiSO4 in 30-day-old seedlings. While decreases were observed in 10 and 100 μM NiSO4 in total soluble protein content in seedlings, increases in POD activity were observed. In addition, it was determined that nickel accumulated especially in the roots. Regarding the effects of nickel on the germination and growth-development of broccoli, it was determined that plant is affected positively in low concentrations (0.01, 0.1 and 1 μM), while high concentrations (10 and 100 μM) caused toxic effects.
Keywords: Nickel, Germination, Plant growth, Broccoli, Brassica oleracea L. var. italica, Metal accumulation

Project Number

2265

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