Determination of the Antifungal Effect of Bacterial Metabolites of Xenorhabdus szentirmaii Against Some Phytopathogenic Fungi

Year 2022, Volume: 10 Issue: 3, 1334 - 1343, 31.07.2022

Nedim Altın , Barış Gülcü

https://doi.org/10.29130/dubited.1012415

Abstract

This study was carried out to determine the antifungal effects of the supernatant produced by the bacterium Xenorhabdus szentirmaii, which is associated with soil-inhabiting entomopathogenic nematodes, on important plant pathogenic fungi, Fusarium verticilliodes, Fusarium oxysporum f.sp lycopersici, Fusarium oxysporum f.sp radicis lycopersici, Botrytis cinerea, Sclerotinia sclerotiorum and Phytophthora nicotianae. The effects of 1, 3, 5 and 7% concentrations of the supernatant produced by X. szentirmaii bacteria on mycelium growth of the fungal pathogens was determined at 3, 7 and 14 days after application (dap). The effects of the supernatant on the formation of sporangia and the emergence of zoospores from the sporangium of Phytophthora nicotianae was also determined. According to the results of the study, the highest dose of the supernatant (7% concentration) prevented mycelium development of all tested pathogens. The effect of the supernatant decreased at 14 dap against F. verticilliodes, F. oxysporum f.sp lycopersici, F. oxysporum f.sp radicis lycopersici. The antifungal effect on S. sclerotiorum completely disappeared in 14 days whereas the effect continued even in 14 days against B. cinerea and P. nicotianae. Xenorhabdus szentirmaii supernatant at a concentration of 7% was highly efficacious on the formation of sporangia and the emergence of zoospores within the sporangium of P. nicotianae at a concentration of 7%.

Keywords

Botrytis, Fusarium, Phytophthora, Sclerotinia, Sporangium, Xenorhabdus.

Thanks

We thank to Dr. Selçuk Hazır from Adnan Menderes University for providing bacteria isolate.

Xenorhabdus szentirmaii Bakteri Metabolitlerinin Bazı Önemli Bitki Patojeni Funguslara Karşı Antifungal Etkilerinin Belirlenmesi

Abstract

Bu çalışmada entomopatojenik nematodların simbiyotik bakterilerinden birisi olan Xenorhabdus szentirmaii tarafından üretilen metabolitlerin önemli bitki patojenlerinden olan Fusarium verticilliodes, Fusarium oxysporum f.sp lycopersici, Fusarium oxysporum f.sp radicis lycopersici, Botrytis cinerea, Sclerotinia sclerotiorum ve Phytophthora nicotianae’ya karşı antifungal etkileri araştırılmıştır. Buna göre farklı oranlarda (%1, 3, 5 ve 7’lik) X. szentirmaii bakteri süpernatantı içeren besiyerlerinde fungusların misel gelişimi 3, 7 ve 14 gün sonra değerlendirilmiştir. Ayrıca supernatantın P. nicotianae’nın sporangia oluşumuna ve zoospor çıkışı üzerindeki etkisi de değerlendirilmiştir. Elde edilen sonuçlara göre test edilen tüm patojenlerde en yüksek süpernatant konsantrasyonu (%7) misel gelişimini önemli oranda engellemiştir. Süpernatantın etkisi F. verticilliodes, F. oxysporum f.sp lycopersici, F. oxysporum f.sp radicis lycopersici’ye karşı 14 gün sonunda azalmıştır. S. sclerotiorum için antifungal etkinin 14 gün sonunda tamamen kaybolduğu, fakat B. cinerea ve P. nicotianae için devam ettiği gözlenmiştir. Ayrıca X. szentirmaii süpernatantının %7’lik konsantrasyonunun P. nicotianae’nın sporangia oluşumu ve zoospor çıkışını engellediği bulunmuştur.           

Keywords

Botrytis, Fusarium, Phytophthora, Sclerotinia, Sporangium, Xenorhabdus

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