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Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay

Year 2022, Volume: 10 Issue: 4, 1664 - 1669, 25.10.2022

Abstract

Imazamox is an imidazolinone herbicide; it is an acetalactacetase (ALS) / acetylhydroxyacid (AHAC) inhibitor and is widely used for weed control. In this study, the genotoxic effect of the commercial formulation of imazamox was investigated in zebrafish peripheral blood cells in vivo using the micronucleus (MN) method. Fishes were treated at three different concentrations of imazamox (3, 6, 12 mg L-1) for 24, 48, 72 and 96 hours. At the highest concentration (12 mg L-1), it was observed that all fish died in all treatment periods. According to 24, 48 and 96 h treatment results; compared to the negative control, it was determined that the MN frequency increased as the concentration increased. These results show us that imazamox herbicide may have genotoxic potential for aquatic organisms. Further studies should be conducted to investigate the mechanisms that are effective in the genotoxicity of imazamox.

Thanks

We thank Mr. Julian Johnson for checking the language of the text as a native speaker.

References

  • [1] S. Ogut, G. N. Cetin, ve E. Kucukoner, “Isparta ve çevre ilçelerde tarımsal üretimde kullanılan pestisitlerin uygulayıcılar üzerindeki akut etkileri,” Toksikoloji Dergisi, c. 6, s. 1–2, ss. 69-72, 2008.
  • [2] A. Zulet, M. Gil-Monreal, J. G. Villamor, A. Zabalza, R. A. L. van der Hoorn, and M. Royuela, “Proteolytic pathways induced by herbicides that inhibit amino acid biosynthesis,” Plos One, vol. 8, no. 9, 2013.
  • [3] X. Ma., Y. Zhang, M. Guan, W. Zhang, H. Tian, C. Jiang, X. Tan and W. Kang, “Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo,” International Journal of Molecular Medicine, vol. 47, no. 103, pp. 1-10, 2021.
  • [4] P. Gupta and S. K. Verma, “Evaluation of genotoxicity induced by herbicide pendimethalin in fresh water fish Clarias batrachus (linn.) and possible role of oxidative stress in induced DNA damage,” Drug and Chemical Toxicology, pp. 1-10, 2020.
  • [5] K. Nagy, R. Argaw, T. Lygia, T. Budnik and B. Ádám, “Comparative cyto- and genotoxicity assessment of glyphosate and glyphosate-based herbicides in human peripheral white blood cells,” Environmental Research, vol. 179, 2019.
  • [6] R. Liman and S. Ozkan, “Cytotoxicity and genotoxicity in Allium cepa L. root meristem cells exposed to the herbicide penoxsulam,” Celal Bayar University Journal of Science, vol. 15, no. 2, pp. 221-226, 2019.
  • [7] B. Ulus, M. Celiksoz and G. Ozhan, “Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-pbutyl herbicides,” Istanbul J Pharm, vol. 47, no. 1, pp. 5-8, 2017.
  • [8] J. M. Pérez-Iglesias, S. Soloneski, N. Nikoloff, G. S. Natale and M. L. Larramendy, “Toxic and genotoxic effects of the imazethapyr based herbicide formulation Pivot H® on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura,Hylidae),” Ecotoxicology and Environmental Safety, vol. 119, pp. 15-24, 2015.
  • [9] U.S. Environmental Protection Agency. (2021, Dec 19). Imazamox; notice of filing a pesticide petition to establish a tolerance for a certain pesticide chemical in or on food. Federal register [Online]. Available: https://www.govinfo.gov/content/pkg/FR-2002-12-23/pdf/02-32260.pdf.
  • [10] FAO/WHO. (2021, Dec 19). Pesticide residues in food. Report 2014, FAO plant production and protection paper [Online]. Available: /https://www.fao.org/3/CB2741EN/bookmarks.pdf
  • [11] European Food Safety Authority (EFSA), “Conclusion on the peer review of the pesticide risk assessment of the active substance imazamox.” EFSA Journal, vol. 14, no. 4:4432, pp. 116, 2016.
  • [12] E. J. Fragiorge, A. A. A. Rezende, U. Graf and M. A. Spano, “Comparative genotoxicity evaluation of imidazolinone herbicides in somatic cells of Drosophila melanogaster,” Food and Chemical Toxicology, vol. 46, pp. 393–401, 2008.
  • [13] C. Sevim, S. Comakli, A. Taghizadehghalehjoughi, M. Özkaraca, R, Mesnage, L. Kovatsi, T. I. Burykina, A. Kalogeraki, M. N. Antoniou and A. Tsatsakis, “An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas,” Toxicology Reports, vol. 6, pp. 42-50, 2019.
  • [14] Y. Liu, Y. Zhang, S. Tao, Y. Guan, T. Zhang, and Z. Wang, “Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure,” Ecotoxicology and Environmental Safety, vol. 130, pp. 124-132, 2016.
  • [15] Massachusetts Department of Agriculture Division of Crop and Pest Services and Massachusetts Department of Environmental Protection Office of Research and Standards. (2021, Dec 19) Imazamox [Online]. Available: https://www.mass.gov/doc/imazamox/download.
  • [16] Thurston County Health Department (2021, Dec 19), Assessment, acute toxıcıty hazard-ecotoxicity, hand, [Online]. Available: https://www.co.thurston.wa.us/Health/ehipm/pdf_terr/terrestrial%20actives/imazamox%20(terrestrial).pdf
  • [17] Ö. Demirci, B. C. Toptanci and M. Kizil, “In vitro studies on pesticide-induced oxidative DNA damage,” JOTCSA, vol. 3, no. 3, pp. 479-490, 2016.

İmazamoks Herbisidinin Zebra Balığı (Danio rerio) Üzerine Olan Genotoksik Etkisinin Mikronukleus Yöntemi Kullanılarak Araştırılması

Year 2022, Volume: 10 Issue: 4, 1664 - 1669, 25.10.2022

Abstract

İmazamoks (IMA), imidazolinon grubunda yer alan, asetalaktatsentaz (ALS) / asetilhidroksiasit (AHAS) inhibitörü olan ve yabani ot kontrolü için yaygın olarak kullanılan bir herbisittir. Bu çalışmada imazamoks’un ticari formülasyonunun genotoksik etkisi zebra balığı periferal kan hücrelerinde in vivo olarak mikronukleus yöntemi kullanılarak araştırıldı. Balıklar imazamoks’un üç farklı konsantrasyonuna (3, 6, 12 mg L-1) 24, 48, 72 ve 96 saatlik boyunca muamele edildiler. En yüksek konsantrasyonda (12 mg L-1) tüm muamele sürelerinde balıkların tamamının öldüğü gözlendi. 24, 48, 72 ve 96 saatlik muamele sonuçları; negatif kontrolle karşılaştırıldığında, konsantrasyon arttıkça MN(mikronükleus) frekansında artış olduğu belirlendi. Bu sonuçlar bize imazamoks herbisitinin sucul canlılar için genoksik potansiyale sahip olabileceğini göstermektedir. İmazamoks’un genotoksisite oluşturmasında etkili olan mekanizmaların araştırılmasına yönelik ileri çalışmalar yapılmalıdır.

References

  • [1] S. Ogut, G. N. Cetin, ve E. Kucukoner, “Isparta ve çevre ilçelerde tarımsal üretimde kullanılan pestisitlerin uygulayıcılar üzerindeki akut etkileri,” Toksikoloji Dergisi, c. 6, s. 1–2, ss. 69-72, 2008.
  • [2] A. Zulet, M. Gil-Monreal, J. G. Villamor, A. Zabalza, R. A. L. van der Hoorn, and M. Royuela, “Proteolytic pathways induced by herbicides that inhibit amino acid biosynthesis,” Plos One, vol. 8, no. 9, 2013.
  • [3] X. Ma., Y. Zhang, M. Guan, W. Zhang, H. Tian, C. Jiang, X. Tan and W. Kang, “Genotoxicity of chloroacetamide herbicides and their metabolites in vitro and in vivo,” International Journal of Molecular Medicine, vol. 47, no. 103, pp. 1-10, 2021.
  • [4] P. Gupta and S. K. Verma, “Evaluation of genotoxicity induced by herbicide pendimethalin in fresh water fish Clarias batrachus (linn.) and possible role of oxidative stress in induced DNA damage,” Drug and Chemical Toxicology, pp. 1-10, 2020.
  • [5] K. Nagy, R. Argaw, T. Lygia, T. Budnik and B. Ádám, “Comparative cyto- and genotoxicity assessment of glyphosate and glyphosate-based herbicides in human peripheral white blood cells,” Environmental Research, vol. 179, 2019.
  • [6] R. Liman and S. Ozkan, “Cytotoxicity and genotoxicity in Allium cepa L. root meristem cells exposed to the herbicide penoxsulam,” Celal Bayar University Journal of Science, vol. 15, no. 2, pp. 221-226, 2019.
  • [7] B. Ulus, M. Celiksoz and G. Ozhan, “Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-pbutyl herbicides,” Istanbul J Pharm, vol. 47, no. 1, pp. 5-8, 2017.
  • [8] J. M. Pérez-Iglesias, S. Soloneski, N. Nikoloff, G. S. Natale and M. L. Larramendy, “Toxic and genotoxic effects of the imazethapyr based herbicide formulation Pivot H® on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura,Hylidae),” Ecotoxicology and Environmental Safety, vol. 119, pp. 15-24, 2015.
  • [9] U.S. Environmental Protection Agency. (2021, Dec 19). Imazamox; notice of filing a pesticide petition to establish a tolerance for a certain pesticide chemical in or on food. Federal register [Online]. Available: https://www.govinfo.gov/content/pkg/FR-2002-12-23/pdf/02-32260.pdf.
  • [10] FAO/WHO. (2021, Dec 19). Pesticide residues in food. Report 2014, FAO plant production and protection paper [Online]. Available: /https://www.fao.org/3/CB2741EN/bookmarks.pdf
  • [11] European Food Safety Authority (EFSA), “Conclusion on the peer review of the pesticide risk assessment of the active substance imazamox.” EFSA Journal, vol. 14, no. 4:4432, pp. 116, 2016.
  • [12] E. J. Fragiorge, A. A. A. Rezende, U. Graf and M. A. Spano, “Comparative genotoxicity evaluation of imidazolinone herbicides in somatic cells of Drosophila melanogaster,” Food and Chemical Toxicology, vol. 46, pp. 393–401, 2008.
  • [13] C. Sevim, S. Comakli, A. Taghizadehghalehjoughi, M. Özkaraca, R, Mesnage, L. Kovatsi, T. I. Burykina, A. Kalogeraki, M. N. Antoniou and A. Tsatsakis, “An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas,” Toxicology Reports, vol. 6, pp. 42-50, 2019.
  • [14] Y. Liu, Y. Zhang, S. Tao, Y. Guan, T. Zhang, and Z. Wang, “Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure,” Ecotoxicology and Environmental Safety, vol. 130, pp. 124-132, 2016.
  • [15] Massachusetts Department of Agriculture Division of Crop and Pest Services and Massachusetts Department of Environmental Protection Office of Research and Standards. (2021, Dec 19) Imazamox [Online]. Available: https://www.mass.gov/doc/imazamox/download.
  • [16] Thurston County Health Department (2021, Dec 19), Assessment, acute toxıcıty hazard-ecotoxicity, hand, [Online]. Available: https://www.co.thurston.wa.us/Health/ehipm/pdf_terr/terrestrial%20actives/imazamox%20(terrestrial).pdf
  • [17] Ö. Demirci, B. C. Toptanci and M. Kizil, “In vitro studies on pesticide-induced oxidative DNA damage,” JOTCSA, vol. 3, no. 3, pp. 479-490, 2016.
There are 17 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Pınar Göç Rasgele 0000-0002-7558-3138

Guncha Meredova 0000-0002-4350-698X

Şerife Gülsün Kırankaya 0000-0002-5171-9256

Publication Date October 25, 2022
Published in Issue Year 2022 Volume: 10 Issue: 4

Cite

APA Göç Rasgele, P., Meredova, G., & Kırankaya, Ş. G. (2022). Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 10(4), 1664-1669. https://doi.org/10.29130/dubited.1050245
AMA Göç Rasgele P, Meredova G, Kırankaya ŞG. Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay. DUBİTED. October 2022;10(4):1664-1669. doi:10.29130/dubited.1050245
Chicago Göç Rasgele, Pınar, Guncha Meredova, and Şerife Gülsün Kırankaya. “Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio Rerio) Using Micronucleus Assay”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 10, no. 4 (October 2022): 1664-69. https://doi.org/10.29130/dubited.1050245.
EndNote Göç Rasgele P, Meredova G, Kırankaya ŞG (October 1, 2022) Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 10 4 1664–1669.
IEEE P. Göç Rasgele, G. Meredova, and Ş. G. Kırankaya, “Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay”, DUBİTED, vol. 10, no. 4, pp. 1664–1669, 2022, doi: 10.29130/dubited.1050245.
ISNAD Göç Rasgele, Pınar et al. “Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio Rerio) Using Micronucleus Assay”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 10/4 (October 2022), 1664-1669. https://doi.org/10.29130/dubited.1050245.
JAMA Göç Rasgele P, Meredova G, Kırankaya ŞG. Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay. DUBİTED. 2022;10:1664–1669.
MLA Göç Rasgele, Pınar et al. “Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio Rerio) Using Micronucleus Assay”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, vol. 10, no. 4, 2022, pp. 1664-9, doi:10.29130/dubited.1050245.
Vancouver Göç Rasgele P, Meredova G, Kırankaya ŞG. Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay. DUBİTED. 2022;10(4):1664-9.