Investigation of Genotoxic Effects of Imazamox Herbicide on Zebrafish (Danio rerio) Using Micronucleus Assay

Year 2022, Volume: 10 Issue: 4, 1664 - 1669, 25.10.2022

Pınar Göç Rasgele , Guncha Meredova , Şerife Gülsün Kırankaya

Abstract

Imazamox is an imidazolinone herbicide; it is an acetalactacetase (ALS) / acetylhydroxyacid (AHAC) inhibitor and is widely used for weed control. In this study, the genotoxic effect of the commercial formulation of imazamox was investigated in zebrafish peripheral blood cells in vivo using the micronucleus (MN) method. Fishes were treated at three different concentrations of imazamox (3, 6, 12 mg L-1) for 24, 48, 72 and 96 hours. At the highest concentration (12 mg L-1), it was observed that all fish died in all treatment periods. According to 24, 48 and 96 h treatment results; compared to the negative control, it was determined that the MN frequency increased as the concentration increased. These results show us that imazamox herbicide may have genotoxic potential for aquatic organisms. Further studies should be conducted to investigate the mechanisms that are effective in the genotoxicity of imazamox.

Keywords

Imazamox, Imidazolinon herbicide, Micronucleus formation

Thanks

We thank Mr. Julian Johnson for checking the language of the text as a native speaker.

İmazamoks Herbisidinin Zebra Balığı (Danio rerio) Üzerine Olan Genotoksik Etkisinin Mikronukleus Yöntemi Kullanılarak Araştırılması

Abstract

İmazamoks (IMA), imidazolinon grubunda yer alan, asetalaktatsentaz (ALS) / asetilhidroksiasit (AHAS) inhibitörü olan ve yabani ot kontrolü için yaygın olarak kullanılan bir herbisittir. Bu çalışmada imazamoks’un ticari formülasyonunun genotoksik etkisi zebra balığı periferal kan hücrelerinde in vivo olarak mikronukleus yöntemi kullanılarak araştırıldı. Balıklar imazamoks’un üç farklı konsantrasyonuna (3, 6, 12 mg L-1) 24, 48, 72 ve 96 saatlik boyunca muamele edildiler. En yüksek konsantrasyonda (12 mg L-1) tüm muamele sürelerinde balıkların tamamının öldüğü gözlendi. 24, 48, 72 ve 96 saatlik muamele sonuçları; negatif kontrolle karşılaştırıldığında, konsantrasyon arttıkça MN(mikronükleus) frekansında artış olduğu belirlendi. Bu sonuçlar bize imazamoks herbisitinin sucul canlılar için genoksik potansiyale sahip olabileceğini göstermektedir. İmazamoks’un genotoksisite oluşturmasında etkili olan mekanizmaların araştırılmasına yönelik ileri çalışmalar yapılmalıdır.

Keywords

İmazamoks, İmidazolinon herbisiti, Mikronukleus oluşumu

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