The Effect of Bellis on Levels of Plasma Paraoxonase Activity, High-Density Lipoprotein and Malondialdehyde in Cyprinus carpio

Year 2022, Volume: 5 Issue: 1, 23 - 29, 30.06.2022

Akram Mudher Kareem Aljubourı H.ahmet Deveci Gökhan Nur

Abstract

The aim of this study is to investigate the effect of Bellis, which is used as a fungicide, on the levels of plasma paraoxonase activity (PON), high-density lipoprotein (HDL), and malondialdehyde (MDA) in Cyprinus carpio. A total of 24 (200-300g) C. carpio fish including eight (8) in each group were used. Fish were divided into three (3) groups; Group-I (control), Group-II (0.025 mg/L Bellis), and Group-III (80.050 mg/L Bellis). Blood samples were collected from the fish after 14-day application and their plasmas were separated. Plasma samples were analysed for PON activity, HDL, and MDA levels. The study findings revealed that PON activity and HDL levels were significantly higher (p<0.001) in Group-I, compared to Group-II and Group-III; whereas, MDA levels were lower (p<0.05). When the dosage of fungicide increased, PON activity and HDL levels dropped but MDA levels increased. In accordance with these findings, it was concluded that Bellis, which has been widely used in recent years, may trigger oxidative stress in fish depending on increasing dose.

Keywords

bellis, Cyprinus carpio, malondialdehyde, oxidative stress, paraoxonase activity

Sazan Balığında Bellis’in Plazma Paraoksonaz Aktivitesi, Yüksek Dansiteli Lipoprotein ve Malondialdehit Düzeyleri Üzerine Etkisi

Abstract

Bu çalışmada fungusit olarak kullanılmakta olan Bellis’in Cyprinus carpio’da plazma paraoksonaz aktivitesi (PON), yüksek dansiteli lipoprotein (HDL) ve malondialdehit (MDA) düzeylerine etkisinin araştırılması amaçlandı. Her grupta 8 adet olmak üzere toplam 24 adet (200-300g) C. carpio balık kullanıldı. Balıklar Grup-I (kontrol), Grup-II (0.025 mg/L Bellis) ve Grup-III 80.050 mgl/L Bellis) olmak üzere 3 gruba ayrıldı. 14 günlük uygulamanın ardından balıklardan kan örneği alındı ve plazmaları ayrıldı. Elde edilen plazma örneklerinde PON aktivitesi, HDL ve MDA düzeyleri çalışıldı. Çalışmada elde edilen bulgulara göre Grup-I’ de PON aktivitesi ve HDL düzeyleri Grup-II ve Grup-III’e göre anlamlı şekilde (p<0.001) daha yüksek bulunurken, MDA düzeylerin ise daha düşük bulundu (p<0.05). Fungisit uygulanan balıklarda artan doza bağlı olarak PON aktivitesi ve HDL düzeyleri azalırken MDA düzeylerinin ise arttığı tespit edildi. Bu sonuçlara göre son yıllarda yaygın bir şekilde kullanılan Bellis’in artan doza bağlı olarak balılarda oksidatif stresi tetikleyebileceği sonucuna varıldı.

Keywords

bellis, Cyprinus carpio, malondialdehit, oksidatif stres, paraoksonaz aktivite

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