Lipid peroxidation and thiol/disulfide homeostasis in cattle with trichophytosis

Abstract

The aim of this study was to determine thiol/disulfide homeostasis in cattle with trichophytosis and to determine the changes in malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px=GPx) levels due to the disease. 15 cattle with trichophytosis and 15 healthy cattle in the control group constituted the material of the study. Blood samples were taken from the jugular vein of the animals in tubes with and without anticoagulant. MDA, CAT, SOD, total thiol, native thiol analyses were performed in serum and GSH-Px analyses were performed in plasma. It was determined that total thiol, native thiol and native thiol/total thiol levels among biochemical parameters in cattle with trichophytosis decreased statistically significantly. It was determined that disulfide/native thiol and disulfide/total thiol levels increased statistically compared to the control group. Although the disulfide level increased compared to the control group, it was not statistically significant. In addition, it was determined that MDA level increased compared to the control group, CAT, SOD and GSH-Px levels decreased statistically significantly compared to the control group. In conclusion, the findings obtained from the study showed that trichophytosis causes oxidative stress in cattle, and the use of oxidative stress markers, especially thiol/disulfide homeostasis markers, would contribute to the pathogenesis of trichophytosis.

Keywords

• Cattle
• oxidative stress
• thiol/disulfide homeostasis
• trichophytosis

Trikofitozisli sığırlarda lipid peroksidasyonu ve tiyol/disülfid homeostazı

Abstract

Amacımız trikofitozisli sığırlarda tiyol/disülfid homeostazisini ortaya koymak ve hastalığa bağlı olarak oluşan malondialdehit (MDA), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px= GPx) seviyesindeki değişimleri belirlemektir. Trikofitozisli 15 ve kontrol grubunu oluşturan 15 adet sağlıklı sığır çalışmanın materyalini oluşturdu. Hayvanların Vena jugularis’inden antikoagulanlı ve antikoagulansız tüplere kan örnekleri alındı. Serumda MDA, CAT, SOD, total tiyol, natif tiyol, plazmada ise GSH-Px analizleri yapıldı. Trikofitozisli sığırlarda total tiyol, natif tiyol ve natif tiyol/total tiyol düzeylerinin istatistiksel olarak anlamlı düzeyde azaldığı belirlendi. Disülfid/natif tiyol ve disülfid/total tiyol düzeylerinin ise kontrol grubuna göre istatistiksel olarak arttığı tespit edildi. Disülfid düzeyi kontrol grubuna göre artmakla beraber istatistiksel olarak anlamsızdı. Bunun yanı sıra MDA düzeyinin kontrol grubuna göre arttığı, CAT, SOD ve GSH-Px düzeylerinin ise kontrol grubuna göre istatistiksel olarak anlamlı düzeyde azaldığı belirlendi. Sonuç olarak çalışmadan elde edilen bulgular trikofitozisin sığırlarda oksidatif strese neden olduğu, oksidatif stres belirteçlerinden özellikle de tiyol/disülfid homeostazis belirteçlerinin kullanımının trikofitozisin patogenezine katkı sağlayacağı kanısına varıldı.

Keywords

• Oksidatif stres
• sığır
• tiyol/disülfid homeostazı
• trikofitozis

References

1. Papini R, Nardoni S, Fanelli A, Mancianti F (2009). High infection rate of Trichophyton verrucosum in calves from central Italy. Zoonoses Public Health, 56:59-64.
2. Lakshmipathy DT, Kannabiran K (2010). Review on dermatomycosis: pathogenesis and treatment. Nat Sci., 2(7):726-731.
3. Bozukluhan K (2014). Deri Hastalıkları. (İçinde): Sığır Hastalıkları. Kırbaş A, Ömür AD, Doğan E (editörler). Baskı 1. Medipres Matbaacılık Ltd Şti, Malatya, Türkiye, 207-216.
4. Paryuni AD, Indarjulianto S, Widyarini S (2020). Dermatophytosis in companion animals: A review. Vet World, 13(6):1174-1181.
5. Merhan O (2022). Biochemistry and antioxidant effects of melatonin. In: Melatonin - Recent Updates. Gelen V, Şengül E and Kükürt A (eds). IntechOpen, London, 44-54.
6. Kahkönen MP, Hopia AI, Vuorela HJ, et al. (1999). Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem., 47:3954-3962.
7. Winterbourn CC, Metodiewa D (1999). Reactivity of biologically important thiol compounds with superoxide and hydrogen peroxide. Free Radic Biol Med., 27(3-4):322-328.
8. Kükürt A, Gelen V, Başer ÖF, Deveci AH, Karapehlivan M (2021). Thiols: role in oxidative stress-related disorders. In: Accenting Lipid Peroxidation. Atukeren P (ed). IntechOpen, London, 27-47.

9. Yoshioka T, Kawada K, Shimada T, Mori M (1979). Lipid peroxidation in maternal and cord blood and protective mechanism againist active-oxygen toxicity in the blood. Am J Obstet Gynecol., 135:372-376.
10. Erel O, Neselioglu S (2014). A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem., 47(18):326-332.
11. Özkanlar YE, Aktas MS, Kirecci, E (2009). Mycozoonozis associated with ringworm of calves in Erzurum province Turkey. Kafkas Univ Vet Fak Derg., 15:141-144.
12. Kabu M, Sayın Z (2016). Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis. Vet Med (Praha), 61(3):133-135.
13. Constable PD, Hinchcliff KW, Done SH, Grünberg W (2017). Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats. 11th ed. Elsevier, China.
14. Sezer K, Keskin M (2014). Serbest oksijen radikallerinin hastalıkların patogenezisindeki rolü. FÜ Sağ Bil Vet Derg., 28:149-156.
15. Apaydin Yildirim B (2020). Evaluation of biochemical parameters and oxidative stress in native and crossbred cattle naturally infected with dermatophytosis. GSC Biol Pharm Sci., 13(02):099-104.
16. Bozukluhan K, Merhan O, Celebi O, Buyuk F, Ogun M, Gokce G (2017). Levels of certain biochemical and oxidative stress parameters in cattle with brucellosis. J Hellenic Vet Med Soc., 68(3):285-290.
17. Bozukluhan K, Merhan O, Gökçe Hİ, et al. (2018). Determination of some acute phase proteins, biochemical parameters and oxidative stress in sheep with naturally infected sheeppox virus. Kafkas Univ Vet Fak Derg., 24(3):437-441.
18. Merhan O, Bozukluhan K, Gokce HI (2017). Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle. J Hellenic Vet Med Soc., 68:535-540.
19. Merhan O, Taşçı GT, Bozukluhan K, Aydın N (2020). Determination of oxidative stress index and total sialic acid in cattle infested with Hypoderma spp. Kafkas Univ Vet Fak Derg., 26(5):633-636.
20. Bozukluhan K, Merhan O, Kiziltepe S, Ergin Egritag H, Akyuz E, Gokce HI (2021). Determination of haptoglobin, some biochemical and oxidative stress parameters in calves with pneumonia. Fresenius Environ Bull., 30:9485-9489.
21. Bayyit E, Merhan O (2020). Normal ve güç doğum yapan ineklerde bazı akut faz proteinlerinin ve oksidatif stres düzeyinin belirlenmesi. Atatürk Üniversitesi Vet Bil Derg., 15(2):145-150.
22. de Zwart LL, Meerman JHN, Commandeur JNM, Vermeulen NPE (1999). Biomarkers of free radical damage applications in experimental animals and in humans. Free Radic Biol Med., 26(1-2):202-226.
23. Arosio B, Gagliano N, Fusaro LMP (2000). Aloe-Emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol&Toxicol., 87:229-233.
24. Kataria N, Kataria AK, Maan R, Gahlot AK (2010). Evaluation of oxidative stress in brucella infected cows. J Stress Physiol Biochem., 6(2):19-25.
25. Beigh SA, Soodan JS, Singh R, Khan AM, Dar MA (2014). Evaluation of trace elements, oxidant/antioxidant status, vitamin C and beta-carotene in dogs with dermatophytosis. Mycoses, 57(6):358-365.
26. Alam RTM, Hassanen EAA, El-Mandrawy SAM (2020). Haemonchus contortus infection in sheep and goats: alterations in haematological, biochemical, immunological, trace element and oxidative stress markers. J Appl Anim Res., 48(1):357-364.
27. Karapehlivan M, Uzlu E, Kaya N, Kankavi O, Ural K, Çitil M (2007). Investigation of some biochemical parameters and the antioxidant system in calves with dermatophytosis. Turk J Vet Anim Sci., 31(2):85-89.
28. Sezer K, Hanedan B, Ozcelik M, Kirbas A (2021). Assessment of oxidative stress, trace elements, serum biochemistry, and hormones levels in weaned calves with dermatophytosis. J Hellenic Vet Med Soc., 72(1):2653-2660.
29. Georgescu SR, Mitran CI, Mitran MI, et al. (2022). Thiol-disulfide homeostasis in skin diseases. J Clin Med., 11(6):1507.
30. Atalay Mert S, Dilbaz B, Kinay T, et al. (2022). Evaluation of oxidative stress with “dynamic thiol/disulfide homeostasis” in cases with endometrioma. Med Sci Discov., 9(8):458-464.
31. Matteucci E, Giampietro O (2010). Thiol signalling network with an eye to diabetes. Molecules, 15:8890-8903.
32. Kuo LM, Kuo CY, Lin CY, Hung MF, Shen JJ, Hwang TL (2014). Intracellular glutathione depletion by oridonin leads to apoptosis in hepatic stellate cells. Molecules, 19:3327-3344.
33. Erdoğan H, Çamkerten İ, Çamkerten G, et al. (2019). The effect of hot-iron disbudding on thiol-disulphide homeostasis in calves. Kafkas Univ Vet Fak Derg., 25(3):335-339.
34. Aydın O, Özkurt G, Çamkerken İ, Eren E, Yanar KE, Aktaş MS (2023). Investigation of ischemia-modified albumin and thiol/disulfide homeostasis for the determination of oxidative stress in sheep with toxoplasmosis. Small Rumin Res., 225:107023.