Investigation of Seroprevalence of Bluetongue Diseases in Sheep in the Province of Siirt

Abstract

Bluetongue is a viral infection that is commonly encountered in domestic and wild ruminants and is transmitted by biting midges. The cause of the disease is within Orbivirus genus of Reoviridae family and has 24 known serotypes. The infection is spread by the flies of Culicoides species. The aim of this study was to determine the serological presence of the bluetongue infection in sheep raised in various locations of Siirt province and to obtain information about the spread of the infection in the region as a whole. The study was conducted between August 2017 and February 2018, and the study consisted of 465 sheep (1-3 years old). 5 mL blood samples were taken from the jugular veins of the sheep to non-anticoagulant tubes for laboratory analyses. The blood samples were centrifuged at 3000 rpm/10 minutes and the serum were transferred to Eppendorf tubes, which were stored at -20 ºC until analysis was carried out. Determination of the Bluetongue-specific antibodies in the serum samples was performed using a commercial test kit by ELISA device. The results of the specific-antibody analysis revealed that 340 (73.12%) of 465 sheep were seropositive, while 125 (26.88%) were seronegative.  As a result, the seroprevalence of Bluetongue disease was found to be considerably high in various locations of Siirt province. Considering the findings of the study, in addition to the measures like the establishment of quarantine protocols and vector controls, utilization of different diagnostic methods and use of vaccines specific to the virus serotypes are suggested to fight the infection. 

Keywords

• Bluetongue,ELISA,seroprevalence,sheep,Siirt

Siirt İli Koyunlarında Mavidil Hastalığı Seroprevalansının Araştırılması

Abstract

Mavidil, evcil ve yabani ruminantlarda görülen ve sokucu sineklerle bulaştırılan viral bir hastalıktır. Hastalığın etkeni, Reoviridae ailesinin Orbivirus cinsi içinde yer alır ve bilinen 24 serotipi bulunmaktadır. Hastalık Culicoides türü sinekler ile yayılmaktadır. Bu araştırma, Siirt ilinin farklı lokalitelerinde yetiştirilen koyunlarda mavidil enfeksiyonun varlığının serolojik olarak belirlenmesi ve yöredeki yaygınlığı hakkında bilgi edinilmesi amacıyla yapılmıştır. Bu çalışma Ağustos 2017- Şubat 2018 tarihleri arasında Siirt ilinin farklı lokalitelerinde yetiştirilen 1-3 yaş aralığında toplam 465 koyun üzerinde gerçekleştirildi. Laboratuvar analizleri için koyunların vena jugularisinden antikoagulansız tüplere 5 mL kan örneği alındı. Alınan örnekler 3000 devirde 10 dakika santrifüje edilip serumları ependorf tüplerine aktarıldı ve analiz yapılıncaya kadar -20 ºC de saklandı. Serum örneklerinde mavidil virüsü spesifik antikor tespiti, ticari test kiti kullanılarak ELISA reader ile gerçekleştirildi. Mavidil virüs antikor tespiti için yapılan analiz sonucunda çalışmanın materyalini oluşturan 465 koyunun 340 (%73.12)’ı seropozitif bulunurken, 125 (% 26.88)’i seronegatif tespit edildi. Sonuç olarak; Siirt ilinin farklı lokalitelerinde mavidil hastalığının seroprevalansı oldukça yüksek tespit edilmiştir. Çalışma verileri dikkate alındığında teşhiste farklı tanı yöntemlerinin kullanılması, hastalıkla mücadelede karantina ve vektör kontrolüne yönelik önlemler yanında, virüs serotiplerine spesifik aşıların kullanılması gerektiği düşünülmektedir.

Keywords

• Koyun,ELISA,Mavidil,Seroprevalans,Siirt

References

1. 1. Saltık HS, Kale M. (2017). Bluetongue Virus Disease. MAKÜ Sag Bil Enst Derg. 5 (1): 32-44.
2. 2. Yıldırım Y, Burgu İ. (2005). The Seroprevalence of Bluetongue (BT), IBR, PI-3, EBL and BVD Infections in Cattle in Northeastern Anatolia. Ankara Üniv Vet Fak Derg. 52: 113-117.
3. 3. Henning M. (1956). Animal Diseases in South Africa. Vol. 3rd. South Africa: Central News Agency Ltd.
4. 4. Wilson AJ, Mellor PS. (2009). Bluetongue in Europe: past, present and future. Philos Trans Royal Soc B. 364 (1530): 2669-2681.
5. 5. Albayrak H, Özan E. (2010). Seroprevalence of Some Arboviral Infections Transported Blood Sucking Insects in Ruminants and Equids in Middle Blacksea. Kafkas Univ Vet Fak Derg. 16 (1): 33-36.
6. 6. Studdert M, Pangborn J, Addison R. (1966). Bluetongue Virus Structure. Virology. 29 (3): 509-511.
7. 7. Mellor P, Wittmann E. (2002). Bluetongue Virus in the Mediterranean Basin 1998–2001. Vet J. 164 (1): 20-37.
8. 8. Kulaç E, Kırmızıgül AH, Yıldırım Y. (2016). Seroprevalance of Bluetongue Infection in Cattle in Rize Region. Atatürk Üniv Vet Bil Derg. 11 (2): 151-158.

9. 9. Purse BV, Mellor PS, Rogers DJ, et al. (2006). Climate change and the recent emergence of bluetongue in Europe. Nat Rev Microbiol. 3: 171-181.
10. 10. Toussaint J, Vandenbussche F, Mast J, et al. (2006). Bluetongue in Northern Europe. Vet Rec. 159 (10): 327.
11. 11. Walton T. (2004). The History of Bluetongue and a Current Global Overview. Vet Ital. 40 (3): 31-38.
12. 12. Tweedle N, Mellor P. (2002). Technical review–bluetongue: The virus, Hosts and Vectors. Veterinary Exotic Disease Divi-sion. DEFRA. Version. 1 21.
13. 13. Darpel KE, Batten CA, Veronesi E, et al. (2009). Transplacental Transmission of Bluetongue Virus in Cattle, UK. Emerg Infect Dis. 15 (12): 2025.
14. 14. Jacquot M, Nomikou K, Palmarini M, Mertens P, Biek R. (2017). Bluetongue Virus Spread in Europe is a Consequence of Climatic, Landscape and Vertebrate Host Factors as Revealed by Phylogeographic Inference. Proc R Soc B. 284 (1864): 20170919.
15. 15. Marku N, Bërxholi K, Spahiu J, Sherifi K, Rexhepi A. (2016). Preliminary Results On Seroprevalence Of Bluetongue In Sheep In Kosovo. Scientific Works Series C. Veterinary Medici-ne. LXII (1):91-93.
16. 16. Meteorology. (2018). Turkish State Meteorological Service. Available from: https://goo.gl/ud7dBK. Access date: 15.09.2017.
17. 17. Yapici O, Bulut O, Avci O, et al. (2014). First Report on Serop-revalence of Bluetongue, Border Disease and Peste Des Petits Ruminants Virus Infections in Sheep in Kyrgyzstan. Indian J Anim Res. 8 (5): 469.
18. 18. Odiawa G, Blue J, Tyler D, Shotts E. (1985). Bluetongue and Epizootic Hemorrhagic Disease in Ruminants in Georgia: Survey by Serotest and Virologic Isolation. Am J Vet Res. 46 (10): 2193-2196.
19. 19. Bitew M, Nandi S, Ravishankar C, Somvanshi R. (2013). Serological and Molecular Evidence of Bluetongue in Sheep and Goats in Uttar Pradesh, India. Afr J Biotechnol. 12 (19): 2699-2705.
20. 20. Di Ventura M, Tittarelli M, Semproni G, et al. (2004). Serologi-al Surveillance of Bluetongue Virus in Cattle, Sheep and Goats in Albania. Vet Ital. 40 (3): 101-104.
21. 21. Mahmoud M, Khafagi MH. (2014). Seroprevalence of Blueton-gue in Sheep and Goats in Egypt. Vet World. 7 (4): 205-208.
22. 22. Oryan A, Amrabadi O, Mohagheghzadeh M. (2014). Seroprevalence of Bluetongue in Sheep and Goats in Southern Iran with an Overview of Four Decades of Its Epidemiological Status in Iran. Com Clin Pathol. 23 (5): 1515-1523.
23. 23. Hasanpour A, Mosakhani F, Mirzaii H, Mostofi S. (2008). Seroprevalence of Bluetongue Virus Infection in Sheep in East-Azerbaijan Province in Iran. Res J Biol Sci. 3 (11): 1265-1270.
24. 24. Gizaw D, Sibhat D, Ayalew B, Sehal M. (2016). Seroprevalence Study of Bluetongue Infection in Sheep and Goats in Selected Areas of Ethiopia. Ethiopian Vet J. 20 (1): 105-114.
25. 25. Yousef MR, Al-Eesa AA, Al-Blowi MH. (2012). High Seropreva-lence of Bluetongue Virus Antibodies in Sheep, Goats, Cattle and Camel in Different Districts of Saudi Arabia. Vet World. 5 (7): 389-393.
26. 26. Ma J-G, Zhang X-X, Zheng W-B, et al. (2017). Seroprevalence and Risk Factors of Bluetongue Virus Infection in Tibetan Sheep and Yaks in Tibetan Plateau, China. BioMed Res Int. 2017:5139703
27. 27. Gür S. (2008). An Investigation of Blue Tongue Virus Infection Using Competative ELISA in Afyonkarahisar province. Vet Bil Derg. 24 (1): 35-39.
28. 28. Özgünlük I. (2003). Seroepidemiological Investigation of Blue-tongue (bt), Akabane (aka) and Ibaraki (iba) Disease in Cattle in Southeastern Project (GAP) area. Ankara Üniv Sağ Bil Enst, Doktora Tezi, Ankara.
29. 29. Savini G, MacLachlan NJ, Sanchez-Vizcaino J-M, Zientara S. (2008). Vaccines Against Bluetongue in Europe. Comp Immunol Microbiol Infect Dis. 31 (2-3): 101-120.
30. 30. Bulut O, Yavru S, Yapkic O, et al. (2006). Serological Investiga-tion of Bluetongue Virus Infection by Serum Neutralization Test and ELISA in Sheep and Goats. Bull Vet Inst Pulawy. 50 (3): 305.
31. 31. Celik O, Sahin T. (2018). Seroprevalence of Bluetongue Disease in the Cattle in Siirt Province, Turkey. J Agric Vet Sci. 11 (12): 39-41.
32. 32. Öztürk F, Yavru S, Eröz S. (1990). The Serological Survey On Bluetongue Virus Infection In Sheep. S Ü Vet Fak Derg. 6 (1): 37-40.
33. 33. Yiğit G. (2009). Serological Investigation of Bluetongue Virus Infection by Enzyme Linked Immunosorbent Assay (Elisa) in Sheep in Konya Region, in Biology (Master thesis). Selcuk University, Institute of Science: Konya.
34. 34. Yılmaz V, Yıldırım Y, Coşkun N. (2015). Serological Investigation of Bluetongue Virus and Rift Valley Fever Virus Infections in Sheep in Kars Province of Turkey. Van Vet J. 26 (3): 119-122.
35. 35. Girgin H, Yonguç A. (1988). Türkiye’deki Koyunların Mavidil Hastalığının Serolojik, Etiyolojik ve Patolojik Durumu Üzerinde Araştırmalar. Etlik Vet Mikrob Derg. 6: 13-24.
36. 36. Özgünlük I, Cabalar M. (2013). Investigation of Bluetongue Virus (BTV Antibodies in Sheep and Goats in Şanlıurfa Province, Turkey. Harran Üniv Vet Fak Derg. 2: 12-17.
37. 37. Uslu U, Dik B. (2004). Seasonal Distribution of Species Culicoides (Diptera: Ceratopogonidae) in Konya Province. Vet Bil Derg. 20 (4): 5-10.
38. 38. Mellor P. (1994). Bluetongue. State Vet J. 4:7-10.