Karnosik ve Gallik Asidin Çözdürme Sonrası Koç Sperm Parametreleri ve Seminal Plazma Homosistein-Nesfatin Düzeyleri Üzerindeki Etkileri

Abstract

Sunulan çalışmada koç spermasının çözüm sonu akrozom ve membran bütünlüğü üzerine karnosik ve gallik asidin koruyucu rolü incelendi, homosistein ve nesfatin düzeyleri belirlendi. Çalışmada her bir koç için altı ejakülat kullanıldı. Beş eşit parçaya bölünen ejakülatlardan, 0.05 mM gallik asit (GA), 2 mM gallik asit, 0.05 mM karnosik asit (CA), 0.2 mM karnosik asit ve katkı maddesi içermeyen (kontrol) gruplar oluşturuldu. Sulandırma işlemi 37 °C'de yapıldı ve 5 ° C de soğutulduktan sonra azot buharında donduruldu. Çözüm sonu koç sperması canlılığı gallik asit 2mM (%57.13±2.38) grubunda, kontrol grubundan (45.08 ±% 2.98) istatistiksel olarak farklı bulundu (p<0.05). Membran bütünlüğü değerlendirmesinde gruplar arasında istatistiksel fark bulunmadı (p> 0.05). En düşük homosistein seviyesi gallik asit gruplarında (0.67±0.11 ve 0.61±0.26 μmol/L) elde edildi ve kontrol grubu (1.36 ± 0.9 μmol/L) ile istatistiksel olarak farklı bulundu (p <0.05). Nesfatin düzeylerinde gruplar arasında anlamlı fark bulunmadı (p> 0.05). Sperma sulandırıcısına GA ilavesinin koç spermasının çözüm sonu canlılığı ve membran bütünlüğü üzerine koruyucu ve aynı zamanda homosistein seviyesini düşürücü etkisi olduğu belirlendi. Nesfatin düzeyinin değerlendirilmesinde gruplar arasında anlamlı fark bulunmadı.

Keywords

• Karnosik asit,Gallik asit,Homosistein,Nesfatin,Sperma

Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing

Abstract

In the presented study, the protective role of carnosic and gallic acid on post-thaw ram sperm acrosome and membrane integrity was examined, homocysteine and nesfatin levels were determined. Six ejaculates for each ram were used in the study. Each ejaculate, split into five equal aliquots was diluted with extenders including 0.05 mM gallic acid (GA), 2 mM gallic acid, 0.05 mM carnosic acid (CA), 0.2 mM carnosic acid and no additive (control) at 37 °C cooled to 5 °C then frozen at nitrogen vapor. Freeze-thawed ram semen viability was achieved in gallic acid 2mM (57.13± 2.38%) group and statistical difference was found with control group (45.08±2.98%) (p<0.05). There was no statistical difference between the groups in membrane integrity evaluation (p>0.05). The lowest level of homocysteine was obtained in the gallic acid groups (0.67±0.11 and 0.61±0.26 μmol/L) and was found statistically different with the control group (1.36±0.9 μmol/L) (p<0.05). No significant difference was found in nesfatin levels among groups (p>0.05). GA supplementation in ram semen extender has been determined to protect viability and membrane integrity also to decrease homocysteine level. There was no significant difference between the groups in the evaluation of nesfatin level.

Keywords

• Carnosic acid,Gallic acid,Homocysteine,Nesfatin,Sperm

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