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Sprague Dawley Sıçanlarında Anne-Yavru Ayrılmasının Maternal Sistolik ve Diyastolik Kan Basıncı Üzerine Etkisi

Year 2021, , 31 - 34, 30.06.2021
https://doi.org/10.47027/duvetfd.839345

Abstract

Bu çalışma, doğum sonrası erken yaşam stresine maruz kalan Sprague Dawley anne sıçanlarda sistolik ve diyastolik kan basıncındaki değişiklikleri araştırmaktadır. On sekiz gebe sıçan rastgele Kontrol (K, n: 6), Anne ayırma (AA, n: 6) ve Kısa Anne Ayırma (KAA, n: 6) gruplarına ayrıldı. Grup K'de (ellenmemiş), anne sıçanlar ve yavrular postnatal 2-14 gün boyunca standart hayvan yetiştirme koşulları altında rahatsız edilmeden barındırıldı. AA grubunda anne sıçanlar ve yavrular postnatal 2. ve 14. günlerde her gün 3 saat birbirinden ayrıldı. Grup KAA'da anne sıçanlar ve yavrular postnatal 2. ve 14. günlerde her gün 15 dk birbirinden ayrıldı. Anne sıçanların sistolik ve diyastolik kan basınçları postnatal 2., 8. ve 14. günlerde tail-cuff yöntemi ile kaydedildi. AA grubunda sistolik kan basıncı tüm postnatal günlerde K grubuna göre daha yüksekti (p<0.001). KAA grubundaki sistolik kan basıncı postnatal 2. ve 8. günde AA grubuna göre sırasıyla daha düşüktü (p<0.01, p<0.001). Ancak postnatal 14. günde AA grubu ile KAA grupları arasında fark yoktu (p>0.05). Diyastolik kan basıncı AA grubunda doğum sonrası tüm günlerde K grubuna göre daha yüksekti (p<0.001, p<0.01, p<0.001). Benzer şekilde doğum sonrası tüm günlerde KAA grubu K grubundan yüksekti (p<0.001, p<0.05, p<0.01). Postnatal 2. günde KAA grubunun diyastolik kan basıncı AA grubuna göre sırasıyla daha düşüktü (p<0.01). Ancak AA grubu ile KAA grupları arasında postnatal 8. ve 14. günlerde fark yoktu (p>0.05). Sonuç olarak, erken yaşam stresi Sprague-Dawley anne sıçanlarda kan basıncını artırabilir.

Supporting Institution

Çalışma hiçbir kurum tarafından desteklenmemiştir.

References

  • 1. Mogi K, Nagasawa M. (2011). Developmental consequences and biological significance of mother-infant bonding. Prog Neuropsychopharmacol Biol Psychiatry. 35: 1232–1241.
  • 2. Okabe S, Nagasawa M, Mogi K. (2012). The importance of mother-infant communication for social bond formation in mammals. Anim Sci J. 83: 446–452.
  • 3. Kristal MB. (2009). The biopsychology of maternal behavior in non-human mammals. ILAR J. 50: 51—63.
  • 4. Cramer CP, Thiels E, Alberts JR. (1990). Weaning in rats: I. Maternal behavior. Dev Psychobiol. 23: 479–493.
  • 5. Lippmann M, Bress A, Nemeroff CB, Plotsky PM, Monteggia LM. (2007). Long-term behavioural and molecular alterations associated with maternal separation in rats. Eur J Neurosci. 25(10): 3091-3098.
  • 6. Gardner KL, Thrivikraman KV, Lightman SL, Plotsky PM, Lowry CA. (2005). Early life experience alters behavior during social defeat: focus on serotonergic systems. Neuroscience. 136(1): 181-91.
  • 7. Plotsky PM, Meaney MJ. (1993). Early, postnatal experience alters hypothalamic corticotropin-releasing factor (CRF) mRNA, median eminence CRF content and stress-induced release in adult rats. Brain Res Mol Brain Res. 18(3): 195-200.
  • 8. Dong M, Giles WH, Felitti VJ, Dube SR, Williams JE, Chapman DP, Anda RF. (2004). Insights into causal pathways for ischemic heart disease: adverse childhood experiences study. Circulation. 110(13): 1761-1766.
  • 9. Levine S. (2005). Developmental determinants of sensitivity and resistance to stress. Psychoneuroendocrinolog. 30: 939–946.
  • 10. Oreland S, Pickering C, Gokturk C, Oreland L, Arborelius L, Nylander I. (2009). Two repeated maternal separation procedures differentially affect brain 5-hydroxytryptamine transporter and receptors in young and adult male and female rats. Brain Res. 1305(Suppl): S37–S49.
  • 11. Boccia ML, Razzoli M, Vadlamudi SP, Trumbull W, Caleffie C, Pedersen CA. (2007). Repeated long separations from pups produce depression-like behavior in rat mothers. Psychoneuroendocrinology. 32: 65–71.
  • 12. Sung YH, Shin MS, Cho S, Baik HH, Jin BK, Chang HK, Lee EK, Kim CJ. (2010). Depression-like state in maternal rats induced by repeated separation of pups is accompanied by a decrease of cell proliferation and an increase of apoptosis in the hippocampus. Neurosci Lett. 470(1): 86-90.
  • 13. Brunton PJ, Russell JA, Douglas AJ. (2008). Adaptive responses of the maternal hypothalamic–pituitary adrenal axis during pregnancy and lactation neuroendocrinology. J Neuroendocrinol.7: 764–76.
  • 14. Kurata A, Morinobu S, Fuchikami M, Yamamoto S, Yamawaki S. (2009). Maternal postpartum learned helplessness (LH) affects maternal care by dams and responses to the LH test in adolescent offspring. Horm Behav. 56: 112–20.
  • 15. van Zyl PJ, Dimatelis JJ, Russell VA. (2016). Behavioural and biochemical changes in maternally separated Sprague-Dawley rat sex posed to restraint stress. Metab Brain Dis. 31(1): 121-33.
  • 16. Daniels WM, Pietersen CY, Carstens ME, SteinDJ. (2004). Maternal separation in rats leads to anxiety-like behavior and a blunted ACTH response and altered neurotransmitter levels in response to a subsequent stressor. Metab Brain Dis. 19(1-2): 3-14.
  • 17. Orso R, Wearick-Silva LE, Creutzberg KC, Centeno-Silva A, Glusman Roithmann L, Pazzin R, Tractenberg SG, Benetti F, Grassi-Oliveira R. (2018). Maternal behavior of the mouse dam toward pups: implications for maternal separation model of early life stress. Stress. 21(1): 19-27.
  • 18. Maniam J, Morris MJ. (2010). Long-term postpartum anxiety and depression-like behavior in mother rats subjected to maternal separation are ameliorated by palatable high fat diet. Behavioural Brain Research. 208: 72–9.
  • 19. Brummelte S, Galea LA. (2010). Depression during pregnancy and postpartum: contribution of stress and ovarian hormones. Prog Neuropsychopharmacol Biol Psychiatry. 34(5): 766–776.
  • 20. Lang PJ, Davis M, Ohman A. (2000). Fear and anxiety: animal models and human cognitive psychophysiology. J Affect Disord. 61: 137–159.
  • 21. DiBona GF, Esler M. (2010). Translational medicine: the antihypertensive effect of renal denervation. Am J Physiol Regul Integr Comp Physiol. 298: R245–R253.
  • 22. Gianaros PJ, Onyewuenyi IC, Sheu LK, Christie IC, Critchley HD. (2012) Brain systems for baroreflex suppression during stress in humans. Hum Brain Mapp. 33: 1700 –1716.
  • 23. Jennings JR, Zanstra Y. (2009). Is the brain the essential in hypertension? Neuroimage. 47(3): 914-921.
  • 24. Gianaros PJ,Sheu LK. (2009). A review of neuroimaging studies of stressor-evoked blood pressure reactivity: emerging evidence for a brainbody pathway to coronary heart disease risk. NeuroImage. 47: 922–936.

The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats

Year 2021, , 31 - 34, 30.06.2021
https://doi.org/10.47027/duvetfd.839345

Abstract

This study investigates changes in systolic and diastolic blood pressure in Sprague Dawley rat dams exposed to postpartum stress. Eighteen pregnant dams were randomly divided into three groups: Control (C, n = 6, no-stress), Maternal Separation (MS, n = 6), and Brief Maternal Separation (BMS, n = 6). The dams and pups in group C were housed under standard conditions in their cages at the animal facility during the postpartum period. From postpartum day 2 to day 14, the dams and pups in group MS and BMS were separated from each other every day for 3-h and 15-min, respectively. Systolic and diastolic blood pressures of the dams were recorded on postpartum days 2, 8, and 14 by tail-cuff method. The findings show that the 3-h dam-pup separation caused high systolic blood pressure in dams, compared to the control dams at all time points that blood pressure was recorded (p<0.001). However, this effect is significant only on postpartum days 2 (p<0.01) and 8 (p<0.001) when compared to the dams that were subjected to 15-min separation. In addition, the 3-h and 15-min dam-pup separations lead to high diastolic blood pressure when compared to the controls throughout the experiment. The 3-h separation caused high diastolic blood pressure only on day 2, when compared to the dams that were subjected to the 15-min pup separation (p<0.01). The obtained data indicate that postpartum dam-pup separation may lead to an increase in blood pressure in Sprague-Dawley rats.

References

  • 1. Mogi K, Nagasawa M. (2011). Developmental consequences and biological significance of mother-infant bonding. Prog Neuropsychopharmacol Biol Psychiatry. 35: 1232–1241.
  • 2. Okabe S, Nagasawa M, Mogi K. (2012). The importance of mother-infant communication for social bond formation in mammals. Anim Sci J. 83: 446–452.
  • 3. Kristal MB. (2009). The biopsychology of maternal behavior in non-human mammals. ILAR J. 50: 51—63.
  • 4. Cramer CP, Thiels E, Alberts JR. (1990). Weaning in rats: I. Maternal behavior. Dev Psychobiol. 23: 479–493.
  • 5. Lippmann M, Bress A, Nemeroff CB, Plotsky PM, Monteggia LM. (2007). Long-term behavioural and molecular alterations associated with maternal separation in rats. Eur J Neurosci. 25(10): 3091-3098.
  • 6. Gardner KL, Thrivikraman KV, Lightman SL, Plotsky PM, Lowry CA. (2005). Early life experience alters behavior during social defeat: focus on serotonergic systems. Neuroscience. 136(1): 181-91.
  • 7. Plotsky PM, Meaney MJ. (1993). Early, postnatal experience alters hypothalamic corticotropin-releasing factor (CRF) mRNA, median eminence CRF content and stress-induced release in adult rats. Brain Res Mol Brain Res. 18(3): 195-200.
  • 8. Dong M, Giles WH, Felitti VJ, Dube SR, Williams JE, Chapman DP, Anda RF. (2004). Insights into causal pathways for ischemic heart disease: adverse childhood experiences study. Circulation. 110(13): 1761-1766.
  • 9. Levine S. (2005). Developmental determinants of sensitivity and resistance to stress. Psychoneuroendocrinolog. 30: 939–946.
  • 10. Oreland S, Pickering C, Gokturk C, Oreland L, Arborelius L, Nylander I. (2009). Two repeated maternal separation procedures differentially affect brain 5-hydroxytryptamine transporter and receptors in young and adult male and female rats. Brain Res. 1305(Suppl): S37–S49.
  • 11. Boccia ML, Razzoli M, Vadlamudi SP, Trumbull W, Caleffie C, Pedersen CA. (2007). Repeated long separations from pups produce depression-like behavior in rat mothers. Psychoneuroendocrinology. 32: 65–71.
  • 12. Sung YH, Shin MS, Cho S, Baik HH, Jin BK, Chang HK, Lee EK, Kim CJ. (2010). Depression-like state in maternal rats induced by repeated separation of pups is accompanied by a decrease of cell proliferation and an increase of apoptosis in the hippocampus. Neurosci Lett. 470(1): 86-90.
  • 13. Brunton PJ, Russell JA, Douglas AJ. (2008). Adaptive responses of the maternal hypothalamic–pituitary adrenal axis during pregnancy and lactation neuroendocrinology. J Neuroendocrinol.7: 764–76.
  • 14. Kurata A, Morinobu S, Fuchikami M, Yamamoto S, Yamawaki S. (2009). Maternal postpartum learned helplessness (LH) affects maternal care by dams and responses to the LH test in adolescent offspring. Horm Behav. 56: 112–20.
  • 15. van Zyl PJ, Dimatelis JJ, Russell VA. (2016). Behavioural and biochemical changes in maternally separated Sprague-Dawley rat sex posed to restraint stress. Metab Brain Dis. 31(1): 121-33.
  • 16. Daniels WM, Pietersen CY, Carstens ME, SteinDJ. (2004). Maternal separation in rats leads to anxiety-like behavior and a blunted ACTH response and altered neurotransmitter levels in response to a subsequent stressor. Metab Brain Dis. 19(1-2): 3-14.
  • 17. Orso R, Wearick-Silva LE, Creutzberg KC, Centeno-Silva A, Glusman Roithmann L, Pazzin R, Tractenberg SG, Benetti F, Grassi-Oliveira R. (2018). Maternal behavior of the mouse dam toward pups: implications for maternal separation model of early life stress. Stress. 21(1): 19-27.
  • 18. Maniam J, Morris MJ. (2010). Long-term postpartum anxiety and depression-like behavior in mother rats subjected to maternal separation are ameliorated by palatable high fat diet. Behavioural Brain Research. 208: 72–9.
  • 19. Brummelte S, Galea LA. (2010). Depression during pregnancy and postpartum: contribution of stress and ovarian hormones. Prog Neuropsychopharmacol Biol Psychiatry. 34(5): 766–776.
  • 20. Lang PJ, Davis M, Ohman A. (2000). Fear and anxiety: animal models and human cognitive psychophysiology. J Affect Disord. 61: 137–159.
  • 21. DiBona GF, Esler M. (2010). Translational medicine: the antihypertensive effect of renal denervation. Am J Physiol Regul Integr Comp Physiol. 298: R245–R253.
  • 22. Gianaros PJ, Onyewuenyi IC, Sheu LK, Christie IC, Critchley HD. (2012) Brain systems for baroreflex suppression during stress in humans. Hum Brain Mapp. 33: 1700 –1716.
  • 23. Jennings JR, Zanstra Y. (2009). Is the brain the essential in hypertension? Neuroimage. 47(3): 914-921.
  • 24. Gianaros PJ,Sheu LK. (2009). A review of neuroimaging studies of stressor-evoked blood pressure reactivity: emerging evidence for a brainbody pathway to coronary heart disease risk. NeuroImage. 47: 922–936.
There are 24 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Mehmet Ekici 0000-0002-2163-6214

Publication Date June 30, 2021
Acceptance Date March 31, 2021
Published in Issue Year 2021

Cite

APA Ekici, M. (2021). The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 14(1), 31-34. https://doi.org/10.47027/duvetfd.839345
AMA Ekici M. The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats. Dicle Üniv Vet Fak Derg. June 2021;14(1):31-34. doi:10.47027/duvetfd.839345
Chicago Ekici, Mehmet. “The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-Gue Dawley Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14, no. 1 (June 2021): 31-34. https://doi.org/10.47027/duvetfd.839345.
EndNote Ekici M (June 1, 2021) The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14 1 31–34.
IEEE M. Ekici, “The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats”, Dicle Üniv Vet Fak Derg, vol. 14, no. 1, pp. 31–34, 2021, doi: 10.47027/duvetfd.839345.
ISNAD Ekici, Mehmet. “The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-Gue Dawley Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14/1 (June 2021), 31-34. https://doi.org/10.47027/duvetfd.839345.
JAMA Ekici M. The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats. Dicle Üniv Vet Fak Derg. 2021;14:31–34.
MLA Ekici, Mehmet. “The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-Gue Dawley Rats”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 14, no. 1, 2021, pp. 31-34, doi:10.47027/duvetfd.839345.
Vancouver Ekici M. The Effect of Maternal-Pup Separation on Maternal Systolic and Diastolic Blood Pressure in Spra-gue Dawley Rats. Dicle Üniv Vet Fak Derg. 2021;14(1):31-4.