Dietilnitrozamin ile İndüklenen Tavşanlarda β-Karotenin Nitrik Oksit ve Malondialdehit Düzeylerine Etkisinin Araştırılması

Öz

Nitrozaminler günlük yaşamda sıklıkla maruz kalınan karsinojenik bileşiklerdendir ve en bilindik üyesi dietilnitrozamin (DEN) olup, hepatik karsinoma neden olmaktadır. Dietilnitrozaminin erken dönem etkileri serbest radikal ve lipit peroksidasyonundaki artışı ile karakterizedir. β-karoten ise DEN’in hepatik hasarlarına karşı koruyucu etkileri olduğu düşünülen bir karotenoid türevidir. Bu çalışmada DEN toksikasyonuna karşı β-karoten uygulamasının serum nitrik oksit (NO) ve malondialdehit (MDA) değerleri üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla 21 Yeni Zelanda ırkı tavşan 3 gruba ayrılmış ve ilk grup kontrol grubu olarak değerlendirilmiştir. Dietilnitrozamin toksikasyonu oluşturulan gruptaki hayvanlara (2. grup) intraperitonal yolla 100 mg/kg oranında DEN uygulanmıştır. Diğer gruptaki hayvanlara (3. grup) ise 100 mg/kg oranında DEN toksikasyonunun ardından 7 gün boyunca 2 mg/kg/gün oranında β-karoten oral olarak verilmiştir. Deneyin 1, 4 ve 7. günlerinde kan örnekleri alınarak serumlarından NO ve MDA değerleri belirlenmiştir. Nitrik oksit seviyeleri β-karoten uygulanan grupta yalnızca DEN uygulanan gruba kıyasla istatistiksel olarak azalmış (P<0.01) ve 1, 4 ve 7. günlerde benzer trendini korumuştur. Ancak 4 ve 7. günlerdeki MDA seviyeleri 1. gün MDA seviyelerine kıyasla artış göstermiştir. Sonuç olarak, β-karotenin DEN toksikasyonunda serum MDA ve NO seviyelerini istatistiksel olarak düşürdüğü görülmüştür. Bu yüzden, DEN’in toksik etkisine karşı β-karotenin NO ve MDA üzerinde koruyucu etkisinin olabileceği kanısına varılmıştır.

Anahtar Kelimeler

• β-karoten
• dietilnitrozamin
• oksidatif stres

The Investigation of the Effect of β-Carotene on Nitric Oxide and Malondialdehyde Levels in Rabbits Induced with Diethylnitrosamine

Abstract

Nitrosamines are carcinogenic compounds that are frequently exposed in daily life and the most well-known member is diethylnitrosamine (DEN) which causing hepatic carcinoma. Early effects of DEN are characterized by the increase in free radical and lipid peroxidation. β-carotene is a carotenoid derivative thought to have protective effects against DEN's hepatic damage. In this study, it was aimed to determine the effects of β-carotene administration against DEN intoxication on serum nitric oxide (NO) and malondialdehyde (MDA) values. For this purpose, 21 New Zealand rabbits were divided into 3 groups and the first group was evaluated as the control group. 100 mg/kg of DEN was administered intraperitoneally to the animals in the group of DEN toxication (2. group). The animals in the other group (3. group) were given-carotene orally at a rate of 2 mg/kg/day for 7 days after 100 mg/kg DEN toxicity. Blood samples were taken on the 1st, 4th and 7th days of the experiment and NO and MDA values were determined from their serum. NO levels decreased in the β-carotene treated group compared to the DEN only group and maintained a similar trend on days 1, 4 and 7. However, MDA levels on days 4 and 7 increased compared to MDA levels on day 1. In conclusion, β-carotene was found to decrease serum MDA and NO levels in DEN intoxication. Therefore, β-carotene may have a protective effect on NO and MDA against toxic effects of DEN.

Keywords

• β-carotene
• diethylnitrosamine
• oxidative stress

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