Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler

Year 2017, Volume: 10 Issue: 1, 1 - 6, 19.02.2017

Birgül Otludil Hülya Karadede Akın

Abstract

Nil tilapiası, Oreochromis niloticus (L.), Cladophora glomerata (L) Kutz (Chlorophyta)’nın bulunup bulunmadığı ortamlarda
kadmiyumun (0,1 mg/l ve 1 mg/l) sublethal konsantrasyonlarına maruz bırakıldı. 15 ve 30 günlük periyotlar sonunda Oreochromis
niloticus karaciğerleri alınarak histolojik preparatları hazırlandı. Kadmiyumun farklı konsantrasyonlarında karaciğerlerde meydana
gelen histopatolojik değişiklikler ışık mikroskobunda incelendi. Kadmiyum uygulaması sonucunda; karaciğer dokusunda, sinüzoidal
bölgelerde dilatasyon, kan damarlarında ve sinüzoidlerde konjesyon, hepatositlerde hipertrofi, hiyalin damlacıkları akümülasyonu,
parankim dejenerasyonu ve lipid vakuolasyonu gibi değişiklikler gözlendi. Ayrıca subkapsüler ve dağınık fokal nekroz görüldü.
Karaciğerlerde lezyonların şiddeti artan kadmiyum konsantrasyonuna ve zamana bağlı olarak artış gösterdi. 

Keywords

Cladophora glomerata, Cadmium, Histopatology, Liver, Oreochromis niloticus

Histological Changes in Liver Tissues of Nile Tilapia Oreochromis niloticus Exposed to Cadmium in the Presence of Green Algae Cladophora glomerata

Abstract

Nile tilapia, Oreochromis niloticus (L.), was exposed to sublethal concentrations (0.1 mg/l and 1 mg/l) of cadmium (cd) in the presence or absence of Cladophora glomerata (L) Kutz (Chlorophyta). At the end of 15 and 30 days periods, Oreochromis niloticus samples were dissected and their livers were collected. Then, the livers were histologically investigated. Histopatological changes occurred in the livers at different concentrations of Cd were studied by light microscope. After Cd application, changes such as dilatation in the sinusoidal region of liver tissue, congestion in blood vessels and sinusoids, hypertrophy in hepatocytes, accumulation of hyaline droplets, degeneration of parenchyma and lipid vacuoiation were observed. Furthermore, subcapsular and dispersed focal necrosis were also encountered. Lesion intensity in the livers increased depending on increased Cd concentration and time. 

Keywords

Cladophora glomerata, Cadmium, Histopatology, Liver, Oreochromis niloticus

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