Rapid Test Kits Used to Identify Antibiotic Residues in Milk and Their Reliability

Year 2018, Volume: 11 Issue: 1, 48 - 54, 30.06.2018

Zeynep Özdemir , Bünyamin Traş

Abstract

Antibacterial drugs are the
most widely use drug group in the veterinary medicine. These drugs pass to the
milk at various levels. There are many commercial kits available for screening
residue in the milk. These kits can be classified as microbiological, immunochemical
and biosensors according to the working principles.  Commercial test kits can give false-positive
results due to the following reasons; the presence of natural antibacterial
substances in colostrum and mastitic milk, high somatic cell count and pH in
mastitis, presence of high fat, fatty acid and protein in milk, detergent and
disinfectant residues in milk, storage and preservative conditions of milk
samples, duration of incubation in microbiological methods, presence of
antiparasitic drug residues in milk, visual interpretation of test results
instead of device. Chromatographic analyse methods are the most reliable method
for identifying and validating residues of antibacterial. Antibiotics and other
chemicals are screened in animal food by the Ministry of Food, Agriculture and
Livestock in Turkey for last 11 years with "Residue Monitoring
Program". 

Keywords

Antibacterial, kit, milk, reliability, residue

Sütte Antibiyotik Kalıntılarının Belirlenmesinde Kullanılan Hızlı Test Kitleri ve Güvenilirlikleri

Abstract

Antibakteriyal
ilaçlar, veteriner klinikte en yaygın kullanılan ilaç grubudur.  Bu ilaçlar çeşitli seviyelerde süte geçerler.
Sütte kalıntı taranmasında kullanılan çok sayıda ticari kit mevcuttur. Bu
kitler çalışma prensiplerine göre mikrobiyolojik, immunokimyasal ve biyosensörler
olarak sınıflandırılır. Ticari test kitlerinde kolostrum ve mastitisli sütte
doğal antibakteriyel maddelerin bulunması, mastitisli sütte yüksek somatik
hücre sayısı ve pH, sütün yüksek oranda yağ, yağ asiti ve protein içermesi,
sütteki deterjan ve dezenfektan kalıntıları, süt numunelerinin depolama ve
saklama koşulları, mikrobiyolojik metotlarda inkübasyon süresi, sütte
antiparaziter ilaç kalıntılarının bulunması, test sonuçlarının cihaz yerine
görsel olarak yorumlanması gibi nedenlere bağlı olarak yanlış-pozitif sonuçlar
alınabilmektedir. Antibakteriyellerin tanımlanması ve doğrulanmasında en
güvenilir yöntem kromatografik metotlardır. Türkiye’de hayvansal gıdalarda
antibiyotikler ve diğer kimyasallar “Kalıntı İzleme Programı” ile son 11 yıldır
Gıda, Tarım ve Hayvancılık Bakanlığı tarafından sürdürülmektedir.

Keywords

Antibakteriyal, güvenilirlik, kalıntı, kit, süt

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