Investigation of Seroprevalence of Bluetongue Diseases in Sheep in the Province of Siirt

Year 2019, Volume: 12 Issue: 1, 53 - 56, 30.06.2019

Özgür Yaşar Çelik , Tekin Şahin

Abstract

Bluetongue is a viral
infection that is commonly encountered in domestic and wild ruminants and is
transmitted by biting midges. The cause of the disease is within Orbivirus
genus of Reoviridae family and has 24 known serotypes. The infection is spread
by the flies of Culicoides species. The aim of this study was to determine the
serological presence of the bluetongue infection in sheep raised in various
locations of Siirt province and to obtain information about the spread of the
infection in the region as a whole. The study was conducted between August 2017
and February 2018, and the study consisted of 465 sheep (1-3 years old). 5 mL
blood samples were taken from the jugular veins of the sheep to
non-anticoagulant tubes for laboratory analyses. The blood samples were
centrifuged at 3000 rpm/10 minutes and the serum were transferred to Eppendorf
tubes, which were stored at -20 ºC until analysis was carried out.
Determination of the Bluetongue-specific antibodies in the serum samples was
performed using a commercial test kit by ELISA device. The results of the
specific-antibody analysis revealed that 340 (73.12%) of 465 sheep were
seropositive, while 125 (26.88%) were seronegative.  As a result, the seroprevalence of Bluetongue
disease was found to be considerably high in various locations of Siirt
province. Considering the findings of the study, in addition to the measures
like the establishment of quarantine protocols and vector controls, utilization
of different diagnostic methods and use of vaccines specific to the virus
serotypes are suggested to fight the infection. 

Keywords

Bluetongue, ELISA, seroprevalence, sheep, Siirt

Siirt İli Koyunlarında Mavidil Hastalığı Seroprevalansının Araştırılması

Abstract

Mavidil, evcil ve yabani
ruminantlarda görülen ve sokucu sineklerle bulaştırılan viral bir hastalıktır.
Hastalığın etkeni, Reoviridae ailesinin Orbivirus cinsi içinde yer alır ve
bilinen 24 serotipi bulunmaktadır. Hastalık Culicoides türü sinekler ile
yayılmaktadır. Bu araştırma, Siirt ilinin farklı lokalitelerinde yetiştirilen
koyunlarda mavidil enfeksiyonun varlığının serolojik olarak belirlenmesi ve
yöredeki yaygınlığı hakkında bilgi edinilmesi amacıyla yapılmıştır. Bu çalışma
Ağustos 2017- Şubat 2018 tarihleri arasında Siirt ilinin farklı lokalitelerinde
yetiştirilen 1-3 yaş aralığında toplam 465 koyun üzerinde gerçekleştirildi.
Laboratuvar analizleri için koyunların vena jugularisinden antikoagulansız
tüplere 5 mL kan örneği alındı. Alınan örnekler 3000 devirde 10 dakika
santrifüje edilip serumları ependorf tüplerine aktarıldı ve analiz yapılıncaya
kadar -20 ºC de saklandı. Serum örneklerinde mavidil virüsü spesifik antikor
tespiti, ticari test kiti kullanılarak ELISA reader ile gerçekleştirildi.
Mavidil virüs antikor tespiti için yapılan analiz sonucunda çalışmanın
materyalini oluşturan 465 koyunun 340 (%73.12)’ı seropozitif bulunurken, 125 (%
26.88)’i seronegatif tespit edildi. Sonuç olarak; Siirt ilinin farklı
lokalitelerinde mavidil hastalığının seroprevalansı oldukça yüksek tespit
edilmiştir. Çalışma verileri dikkate alındığında teşhiste farklı tanı
yöntemlerinin kullanılması, hastalıkla mücadelede karantina ve vektör
kontrolüne yönelik önlemler yanında, virüs serotiplerine spesifik aşıların
kullanılması gerektiği düşünülmektedir.

Keywords

Koyun, ELISA, Mavidil, Seroprevalans, Siirt

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