Bromass’ın Broyler Duodenum Histomorfolojisine Etkileri

Year 2020, Volume: 13 Issue: 2, 108 - 113, 31.12.2020

Sabire Guler , Şule Cengiz , Kerem Atamay

https://doi.org/10.47027/duvetfd.801606

Abstract

Melasın fermantasyonu sonucunda oluşan şilempe, broyler diyetlerinde direk olarak kullanılır. Yeni geliştirilen modifiye şilempe ürünü "Bromass" ın vücut ağırlık artışı, yem alımı ve broyler performans indeksi üzerindeki etkileri bilinirken, Bromass'ın duodenum histomorfolojisi üzerindeki etkileri hakkında bir çalışma yoktur. Bu çalışmada farklı doz Bromass uygulamalarının, duodenum histomorfolojisi; total mukoza, villus yükseklikleri, kript derinliği ve villus yüksekliğinin kript derinliğine oranına etkilerinin araştırılması amaçlanmıştır. Histomorfolojik inceleme için, 40 tane broyler tavuk (Ross-308), 4 gruba ayrılmış, bazal diyet (kontrol) ve Bromass’ın 3 farklı dozuyla (5, 30 ve 60 g/kg) 42 gün boyunca beslenmiştir. Uygulamanın sonucunda, duodenum örnekleri %10’luk formalin içerisinde tespit edilmiş, rutin histolojik prosedür sonrasında Crossman’nın üçlü boyasıyla boyanarak, duodenum mukozasında villus yükseklikleri, kript derinlikleri ve total mukoza ölçümleri yapılmıştır. Broyler tavukların Bromass ile beslenmesi sonrası, duodenumda villus ve total mukoza yüksekliklerinin arttığı gözlendi. Ayrıca sindirilebilirliğin en önemli göstergelerinden olan villus yüksekliğinin kript derinliğine oranında da artış saptandı. Bromass uygulama dozunun artışıyla bu parametrelerde artış belirgin olarak gösterildi. Sonuç olarak, Broyler diyetlerine Bromass katkı maddesinin ilavesi sonucunda duodumum emilim kapasitesinde artış olduğu saptanmıştır ve Bromass’ın ince bağırsak morfolojisi üzerine olumlu etkilerinin olabileceği düşünülmektedir. Bu çalışma temel alınarak, sonraki çalışmalarda, Bromass’ın sindirim sistemindeki farklı etkileri incelenmelidir.

Keywords

Bromass, Broyler, Duodenum

The Effects of Bromass on the Duodenal Histomorphology in the Broilers

Abstract

Molasses fermentation vinasse is used in broiler diets directly. The effects of newly developed modified vinasse product ‘’Bromass’’ on body weight gain, feed intake, and broiler performance index, but there is no study about the Bromass effects on intestinal histomorphology. The objective of present study to examine the different doses’ effects of Bromass on duodenum histomorphology; total mucosa height, villus height, crypt depth, and villus height to crypt depth ratio. For his purpose, 40 broiler chicks (Ross-308) were used and exposed to different doses of Bromass (5, 30, and 60 g/kg) for 42 days. After trials, duodenum sections were fixed with %10 formalin and after rutine histological procedure they were stained with triple (Crossman) stain and villus height, cript depth and total mucosa measured in duodenum. We found that villus height, crypt depth and total mucosa height increase after Bromass supplementation on diets. Also it was determined that villus height to crypt depth ratio, one of the most important parameters showing digestibility was increased. As the dose increased, it was determined that all parameter values increased. As a result of this study, it was found that Bromass supplementation on broiler diets increase absorption capacity and.has positive effects on intestinal morphology. In addition, this study will be lead of future studies is about different effects of Bromass on the digestive system.

Keywords

Bromass, Broiler, Duodenum

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