The ısolation of mastitis agents and determination of sensitive antibiotics in goat milks with subclinical mastitis in Diyarbakır region

Year 2024, Volume: 17 Issue: 2, 100 - 105, 31.12.2024

Simten Yeşilmen Alp , Nihat Özyurtlu , Feray Altan , Servet Bademkıran , Aydın Vural , Yasemin Kaplan Bilmez

https://doi.org/10.47027/duvetfd.1477728

Abstract

There has been an increase in the consumption of goat milk worldwide in recent years, as it is an alternative to cow milk for people with lactose intolerance. Mastitis in goats negatively affects the quality of milk and leads to decreases in milk yield. Therefore, identifying mastitis pathogens is important to prevent infection. The aim of this study is to isolate and identify subclinical mastitis agents in goats in Diyarbakır and its districts and to determine effective antibiotics by the minimum inhibitory concentration (MIC) method. A total of 358 goat milk samples were examined for mastitis with the California Mastitis Test (CMT). In the microbiology examination of a total of 106 milk samples that were CMT (+2, +3) positive, while bacteria were isolated in 79 (74.5%) samples, no growth was observed in 27 (25.5%) samples.. Of the milk samples with bacterial growth, 30 (37.97%) were Staphylococcus aureus, 26 (32.91%) were Coagulase-negative Staphylococci (CNS), 3 (3.80%) were Streptococcus agalactiae, 1 (1.27%) Enterococcus spp., 18 (22.78%) Escherichia coli and 1 (1.27%) Acitenobacter spp. was isolated and identified. Proportional distribution of CNS positive bacteria: 20.25% Staphylococcus xylosus, 5.06% Staphylococcus warneri, 2.53% Staphylococcus lugdunensis, 1.27% Staphylococcus capitis, 2.53% Staphylococcus chromogenes and 1.27% Staphylococcus hominis were identified. According to the MIC results against these bacteria isolated and identified in the study, it was determined that ciprofloxacin, tigecycline, benzylpenicillin, oxacillin and ceftazidimine could be effective in the treatment of mastitis.

Keywords

Goat, identification, isolation, mastitis, prevalence

Diyarbakır bölgesinde subklinik mastitisli keçi sütlerinde mastitis etkenlerinin izolasyonu ve duyarlı antibiyotiklerin belirlenmesi

Abstract

Keçi sütünün, laktoz intoleransı olan insanlarda inek sütüne alternatif olmasından dolayı son yıllarda dünya genelinde tüketiminde artışı görülmektedir. Keçi mastitisi, süt kalitesini olumsuz etkilemekte ve süt veriminde azalmalara yol açmaktadır. Bu nedenle mastitis patojenlerinin belirlenmesi enfeksiyonun önlenmesi açısından önemlidir. Bu çalışmanın amacı Diyarbakır ve ilçelerinde bulunan keçilerde, subklinik mastitis etkenlerinin izolasyonu ve identifikasyonu ile bunlara etkili antibiyotiklerin minimal inhibitör konsantrasyonu (MİK) yöntemi ile belirlenmesidir. Bu amaçla toplam 358 adet keçi süt örneğine California Mastitis Testi (CMT) ile mastitis yönünden bakıldı. CMT (+2,+3) pozitif olan toplam 106 süt örneğinin mikrobiyoloji incelemesinde 79’undan (%74.5) bakteri izole edilirken, 27’sinden (%25.5) herhangi bir üreme gözlenmedi. Üreme olan süt örneklerinin 30’undan (%37.97) Staphylococcus aureus ve 26’sından (%32.91) Koagülaz-negatif Staphylococcus (KNS), 3’ünden (%3.80) Streptococcus agalactiae, 1’inden (%1.27) Enterococcus spp., 18’inden (%22.78) Escherichia coli ve 1’inden de (%1.27) Acitenobacter spp. izole ve identifiye edildi. KNS pozitif olan bakterilerin orantısal olarak dağılımı %20.25 Staphylococcus xylosus, %5.06 Staphylococcus warneri, %2.53 Staphylococcus lugdunensis, %1.27 Staphylococcus capitis, %2.53 Staphylococcus chromogenes ve %1.27 Staphylococcus hominis olarak identifiye edildi. Çalışmada izole ve identifiye edilen bu bakterilere karşı yapılan MİK sonuçlarına göre siprofloksasin, tigesiklin, benzilpenisilin, oksasilin ve seftazidimin mastitis tedavisinde etkili olabileceği belirlendi.

Keywords

İdentifikasyon, izolasyon, keçi, mastitis, prevalans

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