Köpeklerde ve Rhipicephalus sanguineus sensu lato Kenelerinde Hepatozoon canis'in Genetik Çeşitliliği Üzerine Ön Çalışma

Year 2024, Volume: 17 Issue: 2, 118 - 123, 31.12.2024

Sezayi Özübek

https://doi.org/10.47027/duvetfd.1504235

Abstract

Hepatozoon canis, köpeklerde tipik olarak subklinik enfeksiyona neden olan ancak diğer patojenlerle birlikte olduğunda ciddi hastalıklara yol açabilen bir protozoon paraziti, dünya çapında köpeklerde hepatozoonozun birincil sebebidir. Bu çalışma, köpeklerde ve Rhipicephalus sanguineus sensu lato kenelerinde H. canis'in genetik çeşitliliğini biyoinformatik analiz kullanarak araştırmaktadır. Köpeklerden ve kenelerden alınan arşiv DNA örnekleri, 18S rRNA geninin PCR amplifikasyonu ile analiz edilmiş ve ardından BLAST analizi ile dizi karşılaştırması ve biyoinformatik araçlar kullanılarak filogenetik analiz yapılmıştır. Sonuçlar, farklı haplotipleri ayırt etmek için kritik olan çeşitli tek nükleotid polimorfizmlerini (SNP'ler) tanımlayarak genetik değişkenliği ortaya koymuştur. PopART'da oluşturulan Minimum Yayılma Ağları, geniş bir coğrafi dağılıma sahip 18 farklı haplotipi belirlemiştir. Çalışma, H. canis'in geniş genetik çeşitliliğini vurgulamakta ve evrimsel dinamiklerini, bulaşma yollarını, patojenitesini ve direncini anlamak için önemli çıkarımlarda bulunmaktadır. Gelecekteki çalışmalar, H. canis'in genetik yapısını daha ayrıntılı bir şekilde açıklığa kavuşturmak, hedeflenmiş kontrol stratejilerinin geliştirilmesine ve epidemiyolojik bilginin artırılmasına yardımcı olmak için daha değişken genom bölgelerini kullanmalıdır.

Keywords

: Köpek, genetik çeşitlilik, Hepatozoon canis, Rhipicephalus sanguineus sensu lato, 18S rRNA

Preliminary Study on the Genetic Diversity of Hepatozoon canis in Dogs and Rhipicephalus sanguineus sensu lato Ticks

Abstract

Hepatozoon canis, a protozoan parasite, is the primary cause of canine hepatozoonosis worldwide, typically causing subclinical infection in dogs but potentially leading to severe illness when accompanied by other pathogens. This study investigates the genetic diversity of H. canis in dogs and Rhipicephalus sanguineus sensu lato ticks using bioinformatics analysis. Archived DNA samples from dogs and ticks were analyzed through PCR amplification of the 18S rRNA gene, followed by sequence comparison using BLAST analysis and phylogenetic analysis using bioinformatics tools. The results revealed genetic variability, identifying several single nucleotide polymorphisms (SNPs) critical for distinguishing between different haplotypes. Minimum Spanning Networks created in PopART identified 18 distinct haplotypes across a broad geographical distribution. The study highlights the extensive genetic diversity of H. canis, with implications for understanding its evolutionary dynamics, transmission, pathogenicity, and resistance. Future studies should employ more variable genomic regions to further elucidate the genetic landscape of H. canis, aiding in the development of targeted control strategies and enhancing epidemiological knowledge.

Keywords

Bioinformatics, genetic diversity, haplotype, Hepatozoon canis, Rhipicephalus sanguineus sensu lato

References

• Smith TG (1996). The Genus Hepatozoon (Apicomplexa: Adeleina). J Parasitol. 82(4):565–85.
• Hasani SJ, Rakhshanpour A, Enferadi A, Sarani S, Samiei A, Esmaeilnejad BA (2024). Review of Hepatozoonosis Caused by Hepatozoon canis in Dogs. J Parasit Dis. 48(3):424-438
• Little SE, Allen KE, Johnson EM, Panciera RJ, Reichard MV, Ewing SA (2009). New Developments in Canine Hepatozoonosis in North America: A Review. Parasit Vectors. 2(Suppl 1):S5.
• Gabrielli S, Kumlien S, Calderini P, Brozzi A, Iori A, Cancrini G (2010). The First Report of Hepatozoon canis Identified in Vulpes vulpes and Ticks from Italy. Vector Borne Zoonotic Dis. 10(9):855–9.
• Baneth G, Samish M, Shkap V (2007). Life Cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in The Tick Rhipicephalus sanguineus and Domestic Dog (Canis familiaris). J Parasitol. 93(2):283–99.
• Baneth G, Allen K (2022). Hepatozoonosis of Dogs and Cats. (2002). Vet Clin North Am Small Anim Pract. 52(6):1341–58.
• Karagenc TI, Pasa S, Kirli G, et al. (2006). A Parasitological, Molecular and Serological Survey of Hepatozoon canis Infection in Dogs Around The Aegean Coast Of Turkey. Vet Parasitol. 135(2):113–9.
• Orkun Ö, Nalbantoğlu S (2018). Hepatozoon canis in Turkish Red Foxes and Their Ticks. Vet Parasitol Reg Stud Reports. 13:35–7.
• Aktas M, Ozubek S (2017). A Survey of Canine Haemoprotozoan Parasites from Turkey, Including Molecular Evidence of an Unnamed Babesia. Comp Immunol Microbiol Infect Dis. 52:36-42.
• Aktas M, Ozubek S (2017). Transstadial Transmission of Hepatozoon canis by Rhipicephalus sanguineus (Acari: Ixodidae) in Field Conditions. J Med Entomol. 54(4):1044–8.
• Aktas M, Ozubek S, Ipek DNS (2013). Molecular Investigations of Hepatozoon species in Dogs and Developmental Stages of Rhipicephalus sanguineus. Parasitol Res. 112:2381–5.
• Alabí AS, Monti G, Otth C, et al. (2021). Genetic Diversity of Hepatozoon spp. in Rodents from Chile. Rev Bras Parasitol Vet. 30:e012721.
• Inokuma H, Okuda M, Ohno K, Shimoda K, Onishi T (2002). Analysis of The 18S rRNA Gene Sequence of a Hepatozoon Detected in Two Japanese Dogs. Vet Parasitol. 106(3):265–71.
• Tamura K, Stecher G, Kumar S (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 38(7):3022–7.
• Leigh JW, Bryant D (2015). POPART: Full-Feature Software for Haplotype Network Construction. Methods Ecol Evol. 6:1110–1116.
• Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, et al. (2017). DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Mol Biol Evol. 34(12):3299–302.
• Tomé B, Maia JP, Salvi D, et al. (2014). Patterns of Genetic Diversity in Hepatozoon spp. Infecting Snakes From North Africa and the Mediterranean Basin. Syst Parasitol. 87(3):249–58.
• Starkey LA, Panciera RJ, Paras K, et al. (2013). Genetic Diversity of Hepatozoon spp. in Coyotes from The South-Central United States. J Parasitol. 99(2):375–8.
• Junsiri W, Kamkong P, Taweethavonsawat P (2024). First Molecular Detection and Genetic Diversity of Hepatozoon sp.(Apicomplexa) and Brugia sp.(Nematoda) in a Crocodile Monitor in Nakhon Pathom, Thailand. Sci Rep. 14(1):3526.
• de Azevedo Gomes L, Moraes LA, Aguiar DCF, et al. (2018). Genetic Diversity of Hepatozoon spp. in Hydrochoerus hydrochaeris and Pecari tajacu from Eastern Amazon. Ticks Tick-borne Dis. 9(2):314–8.
• Harris DJ, Halajian A, Santos J, Rampedi KM, Xavier R (2019). Genetic Diversity of Hepatozoon (Apicomplexa) from Domestic Cats in South Africa, with a Global Reassessment of Hepatozoon felis Diversity. J S Afr Vet Assoc. 90: 1747.
• Koçkaya ES, Güvendi M, Köseoğlu AE, et al. (2023). Molecular Prevalence and Genetic Diversity of Hepatozoon spp. in Stray Cats of İzmir, Türkiye. Comp Immunol Microbiol Infect Dis.101:102060.
• Kuručki M, Tomanović S, Sukara R, Ćirović D (2022). High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia. Animals. 12(23):3335.
• Helm CS, von Samson-Himmelstjerna G, Liesner JM, et al. (2020). Identical 18S rRNA Haplotypes of Hepatozoon canis in Dogs and Foxes in Brandenburg, Germany. Ticks Tick-borne Dis. 11(6):101520.
• Léveillé AN, Baneth G, Barta JR (2019). Next Generation Sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete Apicoplast Genome And Multiple Mitochondrion-Associated Sequences. Int J Parasitol. 49(5):375–87.