Trikofitozisli sığırlarda lipid peroksidasyonu ve tiyol/disülfid homeostazı

Year 2024, Volume: 17 Issue: 2, 161 - 164, 31.12.2024

Lale Bölükbaşı , Oğuz Merhan

https://doi.org/10.47027/duvetfd.1534484

Abstract

Amacımız trikofitozisli sığırlarda tiyol/disülfid homeostazisini ortaya koymak ve hastalığa bağlı olarak oluşan malondialdehit (MDA), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px= GPx) seviyesindeki değişimleri belirlemektir. Trikofitozisli 15 ve kontrol grubunu oluşturan 15 adet sağlıklı sığır çalışmanın materyalini oluşturdu. Hayvanların Vena jugularis’inden antikoagulanlı ve antikoagulansız tüplere kan örnekleri alındı. Serumda MDA, CAT, SOD, total tiyol, natif tiyol, plazmada ise GSH-Px analizleri yapıldı. Trikofitozisli sığırlarda total tiyol, natif tiyol ve natif tiyol/total tiyol düzeylerinin istatistiksel olarak anlamlı düzeyde azaldığı belirlendi. Disülfid/natif tiyol ve disülfid/total tiyol düzeylerinin ise kontrol grubuna göre istatistiksel olarak arttığı tespit edildi. Disülfid düzeyi kontrol grubuna göre artmakla beraber istatistiksel olarak anlamsızdı. Bunun yanı sıra MDA düzeyinin kontrol grubuna göre arttığı, CAT, SOD ve GSH-Px düzeylerinin ise kontrol grubuna göre istatistiksel olarak anlamlı düzeyde azaldığı belirlendi. Sonuç olarak çalışmadan elde edilen bulgular trikofitozisin sığırlarda oksidatif strese neden olduğu, oksidatif stres belirteçlerinden özellikle de tiyol/disülfid homeostazis belirteçlerinin kullanımının trikofitozisin patogenezine katkı sağlayacağı kanısına varıldı.

Keywords

Oksidatif stres, sığır, tiyol/disülfid homeostazı, trikofitozis

Lipid peroxidation and thiol/disulfide homeostasis in cattle with trichophytosis

Abstract

The aim of this study was to determine thiol/disulfide homeostasis in cattle with trichophytosis and to determine the changes in malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px=GPx) levels due to the disease. 15 cattle with trichophytosis and 15 healthy cattle in the control group constituted the material of the study. Blood samples were taken from the jugular vein of the animals in tubes with and without anticoagulant. MDA, CAT, SOD, total thiol, native thiol analyses were performed in serum and GSH-Px analyses were performed in plasma. It was determined that total thiol, native thiol and native thiol/total thiol levels among biochemical parameters in cattle with trichophytosis decreased statistically significantly. It was determined that disulfide/native thiol and disulfide/total thiol levels increased statistically compared to the control group. Although the disulfide level increased compared to the control group, it was not statistically significant. In addition, it was determined that MDA level increased compared to the control group, CAT, SOD and GSH-Px levels decreased statistically significantly compared to the control group. In conclusion, the findings obtained from the study showed that trichophytosis causes oxidative stress in cattle, and the use of oxidative stress markers, especially thiol/disulfide homeostasis markers, would contribute to the pathogenesis of trichophytosis.

Keywords

Cattle, oxidative stress, thiol/disulfide homeostasis, trichophytosis

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