Urtica dioica L.’da Kuraklık Stresine İlişkin Antioksidant Savunma Sistemi ve ve Fizyolojik Yaklaşımlar

Year 2023, , 84 - 96, 29.12.2023

Hülya Torun

https://doi.org/10.58816/duzceod.1405714

Abstract

Urtica dioicaTürkiye'nin Batı Karadeniz bölgesinin kayalık habitatına ait çok yıllık endemik bir bitkidir. Bu çalışmada, Seseli resinosum Freyn & Sint.’in kuraklığa olan tepkilerini ve tolerans mekanizmasını anlamak için bağıl su içeriği (RWC), klorofil floresansı, prolin birikimi, lipid peroksidasyonu (TBARS), hidrojen peroksit (H2O2) miktarı ve antioksidan enzim miktarındaki değişimleri kuraklık stresini teşvik eden polietilen glikol (PEG) 6000 ( %5, 10 ve 15) varlığında analiz edilmiştir. Araştırma sonucunda, yapraktaki RWC değişmeden kalırken, klorofil floresansı yüksek PEG seviyesi (%15) ile azalmıştır. Ayrıca, PEG uygulamasının artmasıyla H2O2 ve prolin birikimi gözlenmiş, ancak TBARS miktarında artış belirlenmemiştir. Dahası, kuraklık altındaki H2O2 miktarındaki artış, glutatyon redüktaz, katalaz ve süperoksit dismutaz aktivitelerindeki artışa eşlik etmiştir. Diğer taraftan, PEG-teşvikli kuraklık stresi peroksidaz ve askorbat peroksidaz aktivitelerinde azalmaya neden olmuştur. Bu sonuçlar, endemik Urtica dioica bitkisinin kurak şartlar altında antioksidan enzim aktivitelerindeki artışla su durumunu koruyarak etkili bir kuraklık toleransına sahip olduğunu göstermektedir. Bu çalışmada, endemik Seseli resinosum Freyn & Sint.'in fizyolojik ve antioksidatif tepkileri hakkında önemli bilgiler ilk kez ortaya konulmuştur.

Keywords

Antioksidan enzimler, Kuraklık stresi, Urtica dioica, Isırgan

Antioxidant Defense System and Physiological Insights to Drought Stress in Urtica dioica L.

Abstract

Urtica dioicais an endemic perennial plant of rocky habitat of Western Black Sea region of Turkey. To understand drought responses and tolerance mechanism of Seseli resinosum Freyn & Sint., relative water content (RWC), chlorophyll fluorescence, proline accumulation, lipid peroxidation (TBARS), hydrogen peroxide (H2O2) content and changes in antioxidant enzymes were assayed in polyethylene glycol (PEG) 6000 (5, 10 and 15%) induced drought stress in the present study. Leaf RWC maintained unchanged, while chlorophyll fluorescence reduced with high level of PEG (15%). Additionally, H2O2 and proline accumulation were determined with the increase of PEG application, but no increase in the amount of TBARS was determined. Moreover, the increment in H2O2 content under drought was accompanied by increased in glutathione reductase, catalase and superoxide dismutase activities. On the other hand, PEG-induced drought stress caused a reduction in peroxidase and ascorbate peroxidase activities. These results suggest that endemic Urtica dioica plant have an efficient drought tolerance, as displayed by enhanced antioxidant enzyme activities with maintaining water status under drought conditions. In this study, important information about physiological and antioxidative responses of endemic Urtica dioica was revealed for the first time.

Keywords

Antioxidant enzymes, Drought stress, Urtica dioica, Stinging nettle

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