Besin Ortamı Sterilizasyonunda Alternatif Yöntemlerin Gardenia jasminoides’in Mikroçoğaltımındaki Rolü

Year 2024, Volume: 20 Issue: Özel Sayı, 342 - 356, 23.12.2024

Elif Nur Kabadayı , Emrah Zeybekoğlu , John L Griffis, Jr. , Ercan Özzambak

https://doi.org/10.58816/duzceod.1559242

Abstract

Bu çalışmanın amacı gardenyanın mikroçoğaltımında besin ortamlarının sterilizasyonunda geleneksel olarak kullanılan otoklavın yerini alabilecek alternatif sterilizasyon yöntemlerinin geliştirilmesidir. Bu alternatif yöntemlerin, aynı zamanda otoklavlama nedeni ile besin ortamında meydana gelebilecek bazı olumsuz değişiklikleri ortadan kaldırması da beklenmektedir. Çalışmada farklı sürelerde mikrodalga uygulaması ve farklı dozlarda kimyasal dezenfektanlar kullanılmış ve standart otoklavlama yöntemleri ile karşılaştırılmıştır. Tüm kültürler için Gardenia jasminoides sürgünleri kullanılmıştır. Ortalama 6, 8 ve 10 dakika mikrodalga uygulanmıştır. Otoklavlanmış besin ortamında kültüre alınmış sürgünlerin büyümesinin, sürgün uzunluğu, taze ve kuru ağırlık ve sürgün kalitesi açısından 10 dakikalık mikrodalga işlemiyle sterilize edilen ortamda yetiştirilen sürgünlere kıyasla daha iyi olduğu görülmüştür İkinci denemede besin ortamlarında dört farklı dozda NaOCl veya dört dozda NaDCC eklendiğinde kontaminasyon meydana gemediği belirlenmiş, bu çalışmada Kullanılan tüm Na=cl veya düşük konsantrasyonlarda NaDCC ile besin ortamının sterilizasyonunun besin ortamının otoklavlanmasına alternatif bir sterilizasyon yötemi olarak kullanılabileceği belirlenmiştir

Keywords

Mikrodalga sterilizasyon, Otoklavlama, sodyum hipoklorit, Sodyum dikloroizosiyanürat

Thanks

Bu çalışma VIII. Ulusal Süs Bitkileri Kongresi’nde sunulmuştur

The Role of Alternative Methods of Nutrient Medium Sterilization in Micropropagation of Gardenia jasminoides

Abstract

The aim of this study was to develop alternative sterilization methods that could replace the conventionally used autoclave device for sterilization
of nutrient medium used in gardenia micropropagation. These alternative methods may, at the same time, eliminate some negative changes that can occur in nutrient medium because of autoclaving. Either microwave irradiation for differing times or chemical disinfectants in differing doses were utilized as alternative sterilization methods for the nutrient medium. These were compared with standard autoclave methods in the sterilization of the medium. In vitro Gardenia jasminoides shoots were used for all cultures. Microwaves were applied to the medium for 6, 8, and 10 minutes. Growth of gardenia shoots cultured on the autoclaved nutrient medium was significantly better compared to shoots grown on medium sterilized with the 10 minute microwave treatment in terms of shoot length, fresh and dry weight and shoot quality. In the second experiment, no pre cultured contamination occurred in the nutrient media in which either NaOCl was added at four concentrations of 0.002% -0.02% active chlorine or NaDCC was added at four dosages of 25-200 mg /l as sterilization agents. It has been determined that sterilization of nutrient media with either all concentrations of NaOCl or NaDCC at the low concentrations used in this study could be used as an alternative sterilization method to the autoclaving of nutrient media.

Keywords

Microwave sterilization, Autoclaving, Sodium hypochlorite, Sodium dichloroisocyanurate, Contamination

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