Akut Gastroenteritli Çocuklarda Grup A Rota Virus Antijen Sıklığının İmmünokromatik Yöntemle Araştırılması

Year 2017, Volume: 7 Issue: 2, 78 - 81, 24.09.2017

Orhan Akpınar , Hatice Akpınar Esra Şendil Keskin

Abstract

Rotavirüs dünyadaki çocuk ölümlerinin önemli bir faktörüdür ve çocukluk çağında görülen akut gastroenteritin en büyük sebebidir. Rotavirüs enfeksiyonları yatış gerektiren ağır enfeksiyonlar şeklinde görüldüğü gibi hafif enfeksiyon şeklinde de seyredebilir. Mortalite ve morbidite yanında bir takım ekonomik kayıplarada sebeb olabilir. Bu çalışma çocuklarda görülen rotavirüs gastroenteritinin bölgemizdeki epidemiyolojisinin tespiti amacıyla yapılmıştır. Akut gastoenterit şikayeti ile acil kliniğine başvuran 0-14 yaş arası çocuklardan elde edilen dışkı örnekleri rotavirüs açısından değerlendirildi. Üretici firmanın talimatlarına göre Rota virüs A antijeni immünokramatografik yöntemle incelendi. 3102 örnekten 318’inde viral antijenler saptandı (%10.2). Hastaların 179’u (%56.3) erkek, 139’u (%43.7) kadındı. Rotavirüs antijeni en yüksek olarak 2 yaşından küçüklerde gözlemlendi (%44.3; n = 141). Pozitif oran özellikle Ocak, Şubat, Mart ta en yüksek düzeyine ulaşmıştır. Özellikle kış aylarında görülen gastroenteritlerde Rota virüs önemli bir faktör olarak karşımıza çıkmaktadır. Gastroenteritin en büyük sebebi olan rota virüs, dışkıdaki serogrup A antijeninden saptanabilir. İmmünokramatografik yöntemle antijeni hızlı bir şekilde belirlemek mümkündür. Çok kısa sürede dışkıdan antijenin belirleyen ve ishali teşhis eden immünokramatografik yöntemler son derece önemlidir. Gasroenteritli çocuklarda viral faktörlerin hızlı bir şekilde teşhis edilmesinin gereksiz antibiyotik kullanımının önüne geçeceğine inanıyoruz.

Keywords

Akut gastroenterit, immunokramatografik, rotavirüs

Investigation of Group A Rotavirus Antigen Frequency in Children Who Have Acute Gastroenteritis with Immunochromatographic Methods

Abstract

Rotavirus is the major
cause of acute gastroenteritis in infants and young children worldwide and is
the important agent of child deaths. Rotavirus infections represent in
different clinical pictures ranging between mild diarrhea to serious cases
requiring hospitalization. In addition to causing morbidity and mortality in
children, rotavirus gastroenteritis creates a major economic burden on health
care systems and families. To investigate the epidemiology of rotavirus
infections in children with acute gastroenteritis in our region. Feces samples
of 0- to 14-year-old children who were admitted to emergency department with
acute gastroenteritis were examined in terms of rotavirus A antigen with the
immunochromatographic method, using the manufacturer’s instructions. Viral
antigens were detected in 318 of 3102 samples (10.2%). Of the patients, 179
(56.3%) were male and 139 (43.7%) female. The highest rate of rotavirus antigen
positivity was observed in patients younger than 2 years (44.3%; n = 141).
Positivity rate peaked in the January-February-March, especially in winter
months, rotavirus is an important agent in children with acute gastroenteritis.
Diarrhea due to rotavirus, the most common cause, can be detected from
serogroup A antigen in the feces. It is possible to detect with the
immunochromatographic method, which is a rapid testing method.
immunochromatographic methods that can detect antigens from feces and diagnose
diarrhea in a short time period is important. We believe that when patients
present with diarrhea, diagnosed the viral factor accurately and quickly, which
could also prevent unnecessary use of antibiotics.

Keywords

Acute gastroenteritis, immunochromatographic, rotavirus

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