Abstract
The aim of this study is to examine the condroprotective efficacies of intraarticular glucosamine-sulfate administrationsin an experimental osteoarthritis model in rats. There are 2 groups in this study. 15 rats for each group (total 30) wereused in this study. The knee joints of the rats were seen macroscopically and the anterior cruciate ligaments (acl) wereresected. Two weeks after the surgery, three injections were administered intra-articularly once two weeks. 12mg/0.06cc glucosamine-sulfate was injected to the intraarticular cavity to the rats in group 1 (glucosamine-sulphate group). 50ul/0.05 cc 0.9% NaCl solution was injected to the intraarticular cavity to the rats in group 2 (control group). The ratswere sacrificed eight weeks later and the preparations were evaluated histopatologically. In the osteoarthritic rat kneejoints, the histopathological effects of tissue regeneration of glucosamine sulfate and HA applications compared to thecontrol group were shown to be statistically significant. Glucosamine sulphate administration can be accepted as analternative treatment protocol in the treatment of osteoartritis in the knee joint.
References
- Altman RD, Lozada CJ. Clinical features of osteoarthritis. In: Hochberg MC, Silman AJ, Smolen JS, Weinblatt ME, Weisman MH, editors. Rheumatology. 4th ed. Spain: Mosby Elsevier; 2008. p.1703-10.
- Peat G, McCarney R, Croft P. Knee pain and osteoarthritis in older adults: a review of community burden and current use of primary health care. Ann Rheum Dis. 2001; 60:91-7.
- Özkan ÜF, Özkan K, Ramadan S, Güven Z. Chondroprotective effect of N-acetylglucosamine and hyaluronate in early stages of osteoarthritis an experimental study in rabbits. Bulletin of the NYU Hospital for Joint Diseases. 2009; 67(4):352-7.
- Dıraçoğlu D. Osteoartritte intraartiküler hyalüronik asit tedavisi. Türk fiz tıp rehabilitasyon dergisi. 2007; 53:154-9. Doral MN, Dönmez G, Atay ÖA, Bozkurt M, Leblebicioğlu G, Üzümcügil A ve ark. Dejeneratif Eklem Hastalıkları. Totbid Dergisi. 2007; 6:1-2.
- Şen C, Güneş T, Saygı B, Erdem M. Eklem içine uygulanan hyalüronik asidin erken evreli osteoartritte kıkırdak koruyucu etkisi: Tavşanda deneysel çalışma. Acta Orthop Traumatol Turc. 2004; 38(5):348-352.
- Martin CW. Glucosamine: Review of its effectiveness in treating knee osteoarthritis. Workspace BC; January 2004.
- Glucosamine Sulfate. Alternative Medicine Review. Monograph. 1994; 4(3):193-5.
- Horton D, Wander JD. The carbohydrates. Vol IB. New York: Academic Press. 1980.
- Brief AA, Maurer SG, Di Cesare PE. Use of glucosamine and chondroitin sulfate in the management of osteoarthritis. J Am Acad Orthop Surg. 2001; 9:71-8.
- Kazakçıoğlu M. Osteoartrit ve glukozamin. Turk J Rheumatol. 2009; 24:94-7.
- Beaumont GH, Rovari LC. Use of cyrstalline glucosamine sulfate in osteoarthritis. Future rheumatol. 2006; 397-414.
- Chen-ti W, Jinn L, Chee-jen C, Yu-tsan L, Sheng-mou H. Therapeutic effects of hyaluronic acid on osteoarthritis of the knee. J Bone Joint Surg Am. 2004; 86:538-45.
- Gouze JN, Gouze E, Popp MP, Bush ML, Dacanay EA, Kay JD, et al. Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1β. Arthritis Research & Therapy. 2006; 8:R173.
- Mankin HJ, Dorfman H, Lippiello L, Zarins A. Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg. 1971; 53:523-7.
- Murat N, Karadam B, Özkal S, Karatosun V, Gidener S. Sıçanlarda papainle oluşturulan osteoartritin Mankin skoru ile zaman bağlı derecelendirilmesi. Acta Orthop Traumatol Turc. 2007; 41(3):233-7.
- Shikhman AR, Amiel D, D'Lima D, Hwang SB, Hu C, Xu A, et al. Chondroprotective activity of N-acetylglucosamine in rabbits with experimental osteoarthritis. Ann Rheum Dis. 2005; 64(1):89-94.
- Scotto d'Abusco A, Calamia V, Cicione C, Grigolo B, Politi L, Scandurra R. Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes. Arthritis Res Ther. 2007; 9(5):R104.
- Rezende MU, Gurgel HM, Vilaça Junior PR, Kuroba RK, Lopes ASS, Phillipi RZ, et al. Diacerein versus glucosamine in a rat model of osteoarthritis. Clinics. 2006; 61(5):461-6.
Ratlarda Oluşturulan Deneysel Diz Osteoartrit Modelinde İntraartiküler Glukozamin Sülfat Uygulamasının Kondroprotektif Etkisi
Abstract
Bu çalışmada deneysel olarak osteoartrit oluşturulan ratlarda intraartiküler glukozaminsülfat uygulamasının
kondroprotektif etkisinin değerlendirilmesi amaçlanmıştır. Çalışmada 2 grup olup, her grupta 15 olmak üzere toplam
30 rat kullanılmıştır. Ratların dizlerinde ön çapraz bağ makroskopik olarak görülmüş ve eksize edilmiştir. Cerrahi
işlemden 2 hafta sonra her iki gruba da 1 hafta ara ile 3 kez intra artiküler enjeksiyon uygulanmıştır. 1. gruba (glukosamin sülfat grubu) 12mg/0.06cc glukosamin sülfat intraartiküler olarak uygulanmıştır. 2. gruba (kontrol grubu) intraartiküler 50μl/0.05cc %0.9 luk NaCL(Sodyum klorid) uygulanmıştır. Cerrahi sonrası 8. hafta ratlar sakrifiye edilmiştir ve elde edilen preparatlar histopatolojik olarak değerlendirilmiştir. Sonuç olarak osteoartritli ratlarda Glukozamin sülfat uygulamasının kontrol grubuna kıyasla rejenerasyon dokusundaki histopatolojik etkilerinin istatiksel açıdan anlamlı olduğu ortaya konmuştur. Glukozamin sülfatın intraartiküler uygulaması osteoartritli dizlerde uygulanabilecek alternatif bir tedavi yöntemi olarak değerlendirilebilir.
Keywords
References
- Altman RD, Lozada CJ. Clinical features of osteoarthritis. In: Hochberg MC, Silman AJ, Smolen JS, Weinblatt ME, Weisman MH, editors. Rheumatology. 4th ed. Spain: Mosby Elsevier; 2008. p.1703-10.
- Peat G, McCarney R, Croft P. Knee pain and osteoarthritis in older adults: a review of community burden and current use of primary health care. Ann Rheum Dis. 2001; 60:91-7.
- Özkan ÜF, Özkan K, Ramadan S, Güven Z. Chondroprotective effect of N-acetylglucosamine and hyaluronate in early stages of osteoarthritis an experimental study in rabbits. Bulletin of the NYU Hospital for Joint Diseases. 2009; 67(4):352-7.
- Dıraçoğlu D. Osteoartritte intraartiküler hyalüronik asit tedavisi. Türk fiz tıp rehabilitasyon dergisi. 2007; 53:154-9. Doral MN, Dönmez G, Atay ÖA, Bozkurt M, Leblebicioğlu G, Üzümcügil A ve ark. Dejeneratif Eklem Hastalıkları. Totbid Dergisi. 2007; 6:1-2.
- Şen C, Güneş T, Saygı B, Erdem M. Eklem içine uygulanan hyalüronik asidin erken evreli osteoartritte kıkırdak koruyucu etkisi: Tavşanda deneysel çalışma. Acta Orthop Traumatol Turc. 2004; 38(5):348-352.
- Martin CW. Glucosamine: Review of its effectiveness in treating knee osteoarthritis. Workspace BC; January 2004.
- Glucosamine Sulfate. Alternative Medicine Review. Monograph. 1994; 4(3):193-5.
- Horton D, Wander JD. The carbohydrates. Vol IB. New York: Academic Press. 1980.
- Brief AA, Maurer SG, Di Cesare PE. Use of glucosamine and chondroitin sulfate in the management of osteoarthritis. J Am Acad Orthop Surg. 2001; 9:71-8.
- Kazakçıoğlu M. Osteoartrit ve glukozamin. Turk J Rheumatol. 2009; 24:94-7.
- Beaumont GH, Rovari LC. Use of cyrstalline glucosamine sulfate in osteoarthritis. Future rheumatol. 2006; 397-414.
- Chen-ti W, Jinn L, Chee-jen C, Yu-tsan L, Sheng-mou H. Therapeutic effects of hyaluronic acid on osteoarthritis of the knee. J Bone Joint Surg Am. 2004; 86:538-45.
- Gouze JN, Gouze E, Popp MP, Bush ML, Dacanay EA, Kay JD, et al. Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1β. Arthritis Research & Therapy. 2006; 8:R173.
- Mankin HJ, Dorfman H, Lippiello L, Zarins A. Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg. 1971; 53:523-7.
- Murat N, Karadam B, Özkal S, Karatosun V, Gidener S. Sıçanlarda papainle oluşturulan osteoartritin Mankin skoru ile zaman bağlı derecelendirilmesi. Acta Orthop Traumatol Turc. 2007; 41(3):233-7.
- Shikhman AR, Amiel D, D'Lima D, Hwang SB, Hu C, Xu A, et al. Chondroprotective activity of N-acetylglucosamine in rabbits with experimental osteoarthritis. Ann Rheum Dis. 2005; 64(1):89-94.
- Scotto d'Abusco A, Calamia V, Cicione C, Grigolo B, Politi L, Scandurra R. Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes. Arthritis Res Ther. 2007; 9(5):R104.
- Rezende MU, Gurgel HM, Vilaça Junior PR, Kuroba RK, Lopes ASS, Phillipi RZ, et al. Diacerein versus glucosamine in a rat model of osteoarthritis. Clinics. 2006; 61(5):461-6.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | August 28, 2013 |
| Submission Date | August 28, 2013 |
| Published in Issue | Year 2013 Volume: 3 Issue: 1 |
