Abstract
Amaç: Bu çalışmada, Lamiaceae familyasından Salvia verticillata subsp. verticillata L. (Lamiaceae) bitkisinden elde edilen yaprak, kök ve kombine (yaprak+kök) ekstresinin antifungal aktivitesinin araştırılması amaçlandı.
Gereç ve Yöntemler: Bitki örnekleri, Düzce ili, Samandere köyü civarında haziran ayında çiçeklenme döneminde toplanmıştır. Toplanan bitkiler herbaryum tekniklerine göre kurutuldu. Toz haline getirilmiş bitki materyalinin, etil alkol (96%) kullanılarak ekstraksiyonu yapıldı. Tıbbi maya kültürleri (Candida krusei ATCC 20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403 ve Kluyveromyces fragilis ATCC 8608) kullanılarak, görsel sıvı mikrodilüsyon yöntemi ile değerlendirildi.
Bulgular: Çalışmada sonuçları karşılaştırmak için antifungal antibiyotik ketokonazol kullanıldı. MİK değerlerinin 1.56 ile 25.00 mg / mL arasında değiştiği saptandı. Ekstreler, maya kültürlerine karşı güçlü antifungal etki sergilemiştir. Ancak bitki (yaprak+kök) ekstre kombinasyonu, Cryptococcus neoformans, C. laurentii ve Geotrichum capitatum maya kültürlerine karşı daha güçlü antifungal etkiye sahip olduğu belirlenmiştir.
Sonuç: Sonuçlarımız, Salvia verticillata subsp. verticillata ekstrelerinin mantar enfeksiyonlarının tedavisi için geleneksel tıpta kullanımını destekler niteliktedir.
References
- 1. Celep F, Kahraman A. Salvia L. In A. Guner, S. Aslan, T. Ekim, M. Vural, M.T. Babac (Eds.) Türkiye Bitkileri Listesi (Damarli Bitkiler) Nezahat Gokyigit Botanik Bahcesi ve Flora Arastirmalari Dernegi Yayini, Istanbul, 2012.
- 2. Tabanca N, Demirci B, Aytac Z, Baser KHC. The chemical composition of Salvia verticillata L. subsp. verticillata from Turkey. Nat Volatiles & Essent Oils 2017; 4(1):18-28.
- 3. Tabanca N, Demirci B, Baser KHC, Aytac Z, Ekici M, Khan SI, Jacob MR, Wedge DE. Chemical composition and antifungal activity of Salvia macrochlamys and Salvia recognita essential oils. J Agric Food Chem 2006; 54(18):6593-6597. doi: 10.1021/jf0608773
- 4. Ozek G, Demirci F, Ozek T, Tabanca N, Wedge DE, Khan SI, Baser KHC, Duran AH, Hamzaoglu E. Gas chromatographic–mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. J Chromatography A 2010; 1217(5):741-748. https://doi.org/10.1016/j.chroma.2009.11.086
- 5. Askun T, Baser KHC, Tumen G, Kurkcuoglu M. Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turk J Biol 2010; 34(1): 89-95. doi: 10.3906/biy-0809-2
- 6. Suntar I, Akkol EK, Keles H, Oktem A, Baser KHC, Yesilada E. A novel wound healing ointment: a formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacology 2011; 134(1), 89-96. doi: 10.1016/j.jep.2010.11.061
- 7. https://bizimbitkiler.org.tr/yeni/demos/technical/
- 8. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey. Procedia Social and Behavioral Sciences 2011; 19:756-777. https://doi.org/10.1016/j.sbspro.2011.05.195
- 9. Singh H, Gulati IB. Tribological behaviour of some hydrocarbon compounds and their blends. Wear 1990; 139(2): 425-437. https://doi.org/10.1016/0043-1648(90)90060-N
- 10. Dulger B. Antifungal activity of Lamium tenuiflorum against some medical yeast Candida and Cryptococcus species. Pharm Biol 2009; 47(5):467-470. https://doi.org/10.1080/13880200902824881
- 11. Dzoyem JP, Tangmouo JG, Lontsi D, Etoa FX, Lohoue PJ. In vitro antifungal activity of extract and plumbagin from the stem bark of Diospyros crassiflora Hiern (Ebenaceae). Phytotherapy Res 2007; 21(7): 671-674. doi: 10.1002/ptr.2140
- 12. Baser KHC. Aromatic biodiversity among the flowering plant taxa of Turkey. Pure Appl Chem 2002; 74(4): 527-545. doi: 10.1351/pac200274040527
- 13. Altun M, Unal M, Kocagoz T, Goren AC. Essential oil compositions and antimicrobial activity of Salvia species. J Ess Oil Bearing Plants 2007; 10(3): 251-258. doi:10.1080/0972060X.2007.10643550
- 14. Ozkan O, Aydın H, Bagcigil AF. In vitro evaluation of antimicrobial activities of Salvia verticillata and Phlomis pungens. Kafkas Üni Vet Fak Derg 2009; 15(4): 587-590. doi: 10.9775/kvfd.2009.073-A
- 15. Orhan DD, Ozcelik B, Hosbas S, Vural M. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turk J Biol 2012; 36(6): 672-686. doi: 10.3906/biy-1203-33
Abstract
Aim: It was aimed to investigate the antifungal activity of the individual as well as the combine extract (leaf+root) of Salvia verticillata subsp. verticillata L. from Lamiaceae family which has ethnobotanical use.
Material and Methods: The plant samples were collected during to flowering stage in June, Samandere village around, Duzce, Turkey. The collected plants were dried according to herbarium techniques. The powdered plant material was extraction using ethyl alcohol (96%). Antifungal effects of the extracts was evaluated for yeasts (Candida krusei ATCC 20298, C. parapsilosis ATCC 90112, C. guilliermondii ATCC 6260, C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. glabrata ATCC 2001, Cryptococcus laurentii ATCC 34142, C. neoformans ATCC 90112, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608) by visual broth microdilution method. Antifungal antibiotic ketoconazole was used in the study to compare the results.
Results: MIC values ranged from 1.56 to 25.00 mg/mL. The extracts have shown potential antifungal effects against the yeast cultures. Notably, the combine plant extracts (both leaves and rootstock) possessed higher antifungal potency against C. neoformans, C. laurentii and G. capitatum.
Conclusion: Our results demonstrated that the extracts of Salvia verticillata subsp. verticillata are used for the treatment of fungal infections as traditional medicine
References
- 1. Celep F, Kahraman A. Salvia L. In A. Guner, S. Aslan, T. Ekim, M. Vural, M.T. Babac (Eds.) Türkiye Bitkileri Listesi (Damarli Bitkiler) Nezahat Gokyigit Botanik Bahcesi ve Flora Arastirmalari Dernegi Yayini, Istanbul, 2012.
- 2. Tabanca N, Demirci B, Aytac Z, Baser KHC. The chemical composition of Salvia verticillata L. subsp. verticillata from Turkey. Nat Volatiles & Essent Oils 2017; 4(1):18-28.
- 3. Tabanca N, Demirci B, Baser KHC, Aytac Z, Ekici M, Khan SI, Jacob MR, Wedge DE. Chemical composition and antifungal activity of Salvia macrochlamys and Salvia recognita essential oils. J Agric Food Chem 2006; 54(18):6593-6597. doi: 10.1021/jf0608773
- 4. Ozek G, Demirci F, Ozek T, Tabanca N, Wedge DE, Khan SI, Baser KHC, Duran AH, Hamzaoglu E. Gas chromatographic–mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. J Chromatography A 2010; 1217(5):741-748. https://doi.org/10.1016/j.chroma.2009.11.086
- 5. Askun T, Baser KHC, Tumen G, Kurkcuoglu M. Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turk J Biol 2010; 34(1): 89-95. doi: 10.3906/biy-0809-2
- 6. Suntar I, Akkol EK, Keles H, Oktem A, Baser KHC, Yesilada E. A novel wound healing ointment: a formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacology 2011; 134(1), 89-96. doi: 10.1016/j.jep.2010.11.061
- 7. https://bizimbitkiler.org.tr/yeni/demos/technical/
- 8. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey. Procedia Social and Behavioral Sciences 2011; 19:756-777. https://doi.org/10.1016/j.sbspro.2011.05.195
- 9. Singh H, Gulati IB. Tribological behaviour of some hydrocarbon compounds and their blends. Wear 1990; 139(2): 425-437. https://doi.org/10.1016/0043-1648(90)90060-N
- 10. Dulger B. Antifungal activity of Lamium tenuiflorum against some medical yeast Candida and Cryptococcus species. Pharm Biol 2009; 47(5):467-470. https://doi.org/10.1080/13880200902824881
- 11. Dzoyem JP, Tangmouo JG, Lontsi D, Etoa FX, Lohoue PJ. In vitro antifungal activity of extract and plumbagin from the stem bark of Diospyros crassiflora Hiern (Ebenaceae). Phytotherapy Res 2007; 21(7): 671-674. doi: 10.1002/ptr.2140
- 12. Baser KHC. Aromatic biodiversity among the flowering plant taxa of Turkey. Pure Appl Chem 2002; 74(4): 527-545. doi: 10.1351/pac200274040527
- 13. Altun M, Unal M, Kocagoz T, Goren AC. Essential oil compositions and antimicrobial activity of Salvia species. J Ess Oil Bearing Plants 2007; 10(3): 251-258. doi:10.1080/0972060X.2007.10643550
- 14. Ozkan O, Aydın H, Bagcigil AF. In vitro evaluation of antimicrobial activities of Salvia verticillata and Phlomis pungens. Kafkas Üni Vet Fak Derg 2009; 15(4): 587-590. doi: 10.9775/kvfd.2009.073-A
- 15. Orhan DD, Ozcelik B, Hosbas S, Vural M. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turk J Biol 2012; 36(6): 672-686. doi: 10.3906/biy-1203-33
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | September 20, 2021 |
| Submission Date | February 8, 2021 |
| Published in Issue | Year 2021 Volume: 11 Issue: 3 |
