Effect of Enamel Preperation Methods on Microleakage of Two Different Fissure Sealants

Abstract

This in-vitro study compared the microleakage of unfilled and filled resin–based fissure sealant materials, after acid etched and Er:YAG laser treated enamel. Extracted sound human third molars were randomly assigned into 6 groups n=10/each . The occlusal surfaces were sealed with an unfilled Clinpro TM, 3M ESPE and filled Helioseal F, Ivoclar Vivadent resin-based fissure sealant material after one of the following pretreatments; 1. phosphoric acid etching only 30 sc ; 2. Er:YAG laser 240 mJ, 10 Hz, 2940nm wavelength ; 3. Er:YAG laser + acid etching 15 sc . Teeth were thermal cycled from 5-55 0C 1000 times with 15 sc dwell time at each temperature. Teeth were sectioned bucco-lingually and stained with basic fuchsin. Microleakage was evaluated at X50 optical magnification with a stereo-microscope. Data were statistically analysed with Kruskal-Wallis test. In this study, microleakage was found to be related to the enamel surface conditioning rather than the filler ratio of the material. Marginal leakage of the filled resin sealant was found to be similar with the unfilled resin group p>0.05 . The microleakage in Er:YAG laser group displayed greater microleakage than the other groups p 0.05 . Er:YAG laser irradiation did not eliminate the need for the acid etching of the enamel surface before sealant application

Keywords

• Pit and fissure sealant, ER:YAGlaser, microleakage

Mine yüzeyi hazırlama tekniklerinin iki farklı fissür örtücünün mikrosızıntısına etkisi

Abstract

In–vitro çalışmamızda, üç farklı mine yüzeyihazırlama tekniğinin, farklı doldurucu oranlarınasahip rezin esaslı fissür örtücülerin mikrosızıntısına etkisinin araştırılması amaçlanmıştır. Çalışmada, 60 adet çekilmiş çürüksüz yirmi yaş dişi kullanılmıştır n=10 . Mine yüzeylerine; 1. grupta;%37’ lik fosforik asit FGM, Condec 37,USA 30sn, 2. grupta; sadece Er:YAG lazer 240 mJ, 10 Hz,2940nm dalga boyu , 3.grupta; lazeri takiben asit15sn uygulanmıştır. Yıkanıp kurutulan mine yüzeylerine rezin içerikli farklı doldurucu oranlarınasahip iki ayrı fissür örtücü Clinpro TM, 3M ESPE;Helioseal F, Ivoclar Vivadent uygulanarak LEDışık kaynağıyla polimerize edilmiştir. 1000 kez 5/550C termal siklusa tabi tutulan dişler bukkolingual yönde ikiye ayrılarak, bazik fuksinle boyasızıntı testine tabi tutulmuştur. Mikrosızıntı X50büyültmede değerlendirilmiştir. Veriler KruskalWallis Test ile karşılaştırılmıştır. Çalışmamızdamikrosızıntının, fissür örtücülerin yüzey hazırlamatekniği ile ilişkili olarak şekillendiği gözlenmiştir.Lazer grubundaki mikrosızıntı değerleri, asit ve lazeri takiben asit uygulanan gruplara oranla anlamlı şekilde yüksek bulunmuştur p< 0.05 . Asit ile lazer+asit uygulanan gruplar arasındaki fark ise anlamlı değildir p> 0.05 . Çalışmanın sonucundahem dolduruculu hem de doldurucusuz resin esaslıfissür örtücülerde mineye tek başına uygulananEr:YAG lazerin mikrosızıntıyı olumsuz yönde etkilediği bulunmuştur. Lazeri takiben uygulanan asidin, mikrosızıntı açısından sadece asit uygulamasına oranla ilave bir yarar sağlamadığı sonucunavarılmıştır

Keywords

• Pit ve fissür örtücü,ER:YAG lazer, mikrosızıntı

References

1. Simonsen RJ. Retention and effectiveness of dental sealant after 15 years. J Am Dent Assoc 1991; 122:34-42.
2. Subramaniam P, Babu KL, Naveen HK. Effect of tooth preparation on sealant success--an in vitro study. J Clin Pediatr Dent 2009; 33:325-31.
3. Walsh LJ. Split-mouth study of sealant reten- tion with carbon dioxide laser versus acid etch con- ditioning. Aust Dent J 1996; 41:124-7.
4. Pelagalli J, Gimbel CB, Hansen RT, Sweet A, Winn DW. Investigation study of the use of Er:YAG laser versus dental drill for caries removal and cavity praparation-phase I. J Clin Laser Med Surg 1997; 15:109-15.
5. Cozean C, Arcoria CJ, Pelagalli J, Powell GL. Dentistry for 21st century Erbium:YAG laser for teeth. J Dent Res 1997; 128:1080-8
6. Roebuck EM, Sounders WP, Whitters CJ. Influence of three Erbium:YAG laser energies on the in vitro microleakage of Class V resin-based com- posite restorations. Am J Dent 2000; 13: 280-9.
7. Hibst R, Keller U. Experimental studies of the application of the Er:YAG laser on dental hard substances: I. Measurement of the ablation rate. Lasers Surg Med 1989; 9:338-44.
8. Keller U, Hibst R. Experimental studies of the application of the Er:YAG laser on dental hard substances: II. Light microscopic and SEM investi- gations. Lasers Surg Med 1989; 9:345-51.

9. Zakariasen KL, MacDonald R, Boran T. Spotlight on lasers. A look at potential benefits. J Am Dent Assoc 1991; 122:58-62.
10. Walsh LJ. Split-mouth study of sealant retention with carbon dioxide laser versus acid etch conditioning. Aust Dent J 1996;41:124-7.
11. Pardi V, Sinhoreti MA, Pereira AC, Ambrosano GM, Meneghim Mde C. In vitro evalu- ation of microleakage of different materials used as pit-and-fissure sealants. Braz Dent J 2006;17:49-52.
12. Martínez-Insua A, Da Silva Dominguez L, Rivera FG, Santana-Penín UA. Differences in bon- ding to acid-etched or Er:YAG-laser-treated enamel and dentin surfaces. J Prosthet Dent 2000; 84:280-8.
13. Borsatto MC, Corona SA, Dibb RG, Ramos RP, Pécora JD. Microleakage of a resin sealant after acid-etching, Er:YAG laser irradiation and air-abra- sion of pits and fissures. J Clin Laser Med Surg 2001; 19:83-7.
14. Lupi-Pegurier L, Bertrand MF, Muller- Bolla M, Rocca JP, Bolla M. Comparative study of microleakage of a pit and fissure sealant placed after preparation by Er: Yag laser in permanent molars. J Dent Child 2003; 70:134-8.
15. De Craene GP, Martens C, Dermaut R. The invasive pit-and-fissure sealing technique in pedi- atric dentistry: a SEM study of a preventive restora- tion. ASDC J Dent Child 1988; 55:34-42.
16. Barnes&Others. Flow characteristics and sealing ability of fissure sealants. Op Dent 2000; 25:306-10.
17. Barrie AM, Stephen KW, Kay EJ. Fissure sealant retention: a comparison of three sealant types under field conditions. Community Dent Health 1990; 7:273-7.
18. Rock WP, Weatherill S, Anderson RJ. Retention of three fissure sealant resins. The effects of etching agent and curing method. Results over 3 years. Br Dent J 1990; 168:323-5.
19. Futatsuki M, Kubota K, Yeh YC, Park K, Moss SJ. Early loss of pit and fissure sealant: a cli- nical and SEM study. J Clin Pediatr Dent 1995; 19:99-104.
20. Tulga F, Kara D. Farklı yüzey hazırlama tekniklerinin ve asitleme sürelerinin fissür örtücü- lerin bağlanma kuvvetleri üzerine etkilerinin süt dişlerinde değerlendirilmesi (Bölüm II). GÜ Diş Hek. Fak. Derg 1998;15:41-50.
21. Lupi-Pégurier L, Bertrand MF, Genovese O, Rocca JP, Muller-Bolla M. Microleakage of resin- based sealants after Er:YAG laser conditioning. Lasers Med Sci 2007; 22:183-8.
22. Kato J, Moriya K, Jayawardena JA, Wijeyeweera RL. Clinical application of Er:YAG laser for cavity preparation in children. J Clin Laser Med Surg 2003; 21:151-5.
23. Hoke JA, Burkes EJ, Gomes ED, Wolbarsht ML. Erbium:YAG (2,94µm)laser effects on dental tissues. J Laser Appl 1990; 2:61-5.
24. Visuri SR, Walsh JT Jr, Wigdor HA. Erbium laser ablation of dental hard tissue: effect of water cooling. Lasers Surg Med 1996; 18:294-300.
25. Groth EB, Mercer CE, Anderson P. Microtomographic analysis of subsurface enamel and dentine following Er:YAG laser and acid etching. Eur J Prosthodont Restor Dent 2001; 9:73-9
26. Shortall AC. Microleakage, marginal adap- tation and composite resin restorations. Br Dent J 1982; 153:223-7.
27. Wendt SL, McInnes PM, Dickinson GL. The effect of thermocycling in microleakage analysis. Dent Mater 1992; 8:181-4.
28. Xalabarde A, Garcia-Godoy F, Boj JR, Canaida C. Fissure micromorphology and sealant adaptation after occlusal enameloplasty J Clin Pediatr Dent 1996; 20:299-304.
29. Kalachandra S, Kusy RP. Comparison of water sorption by methacrylate and dimethacrylate monomers and their corresponding polymers. Polymer 1991; 32:2428-34.
30. Kalachandra S, Taylor DF, Deporter CD, Grubbs HJ, McGrath JE. Polymeric materials for composite matrixes in biological environments. Polymer 1993; 34:778-82.
31. A.J. Feilzer, B.S. Dauvillier. Effect of TEGDMA/BisGMA ratio on stress development and viscoelastic properties of experimental two-paste composites. J Dent Res 2003; 82:824-8.
32. Koch MJ, García-Godoy F, Mayer T, Staehle HJ. Clinical evaluation of Helioseal F fissure sealant. Clin Oral Investig 1997; 1:199-202.
33. Waggoner WF, Siegal M. Pit and fissure sealant application: updating the technique. J Am Dent Assoc 1996; 127:351-61.
34. Feldens EG, Feldens CA, de Araujo FB, Souza MA. Invasive technique of pit and fissure sealants in primary molars: a SEM study. J Clin Pediatr Dent 1994; 18:187-90.
35. Park K, Georgescu M, Scherer W, Schulman A. Comparison of shear strength, fracture patterns, and microleakage among unfilled, filled, and fluoride-releasing sealants. Pediatr Dent 1993; 15:418-21.
36. Wright JT, Retief DH. Laboratory evalu- ation of eight pit and fissure sealants. Pediatr Dent 1984; 6:36-40.
37. Ramos RP, Pecora JD, Brugnera AJ, Corona SAM, Palma Dibb RG. Morphological analysis of dental surface treated by two Er:YAG laser devices. J Dent Res 2002; 81(spec issue B):B- 181-5.
38. Borges DG, Watanabe I, Brugnera A. A SEM comparison of Er:YAG pulsed and CO2 super- pulsed lasers on decidious teeth enamel. J Dent Res 1999; 8: 496-9.
39. Moshonov J, Stabholz A, Zyskind D, Sharlin E, Peretz B. Acid-etched and erbium:yittrium aluminium garnet laser-treated enamel for fissure sealants: a comparison of microleakage. Int J Paediatr Dent 2005; 15:205-9.