Monosodyum İodoasetat ile İndüklenen Diz Osteoartriti Modellerinde Bitki Bazlı Tedavilerin Araştırılması

Abstract

Diz osteoartriti, diz ekleminde histolojik ve biyokimyasal değişikliklere yol açan bir eklem bozukluğudur. Diz osteoartriti tedavisinde farmakolojik ve farmakolojik olmayan yöntemler uygulanmaktadır. Farmakolojik olmayan yöntemler arasında bitkisel uygulamalar da yer almaktadır. Monosodyum iodoasetat (MIA) uygulaması, diz osteoartriti çalışmalarında sıklıkla kullanılan bir yöntemdir. Bu modellere yönelik çalışmalar, diz bölgesindeki ağrı ve iltihabı azaltmayı, apoptozu önlemeyi ve osteoartritin ilerlemesini yavaşlatmayı amaçlamaktadır. Bu model uygulanırken, bitki ve bitki bazlı uygulamalar da analiz edilmektedir. Derlememizde, monosodyum iodoasetat ile indüklenen diz osteoartriti modellerinde uygulanan bitki ve bitki bazlı tedaviler; kullanılan bitkiler, uygulama şekli, türler, kullanılan MIA miktarı, çözücü, MIA uygulamasından sonra kesim günü ve etkilediği yollar (pathways) açısından ele alınmıştır. Bu çalışmaların derlenmesiyle, preklinik tartışmalara katkı sağlanması amaçlanmaktadır.

Keywords

• osteoartirit
• bitki
• tedaviler
• monosodyum iyodoasetat

Investigation of Plant-Based Treatments, Mechanisms of Action, and Related Molecular Pathways in Models of Monosodium Iodoacetate Induced Knee Osteoarthritis: A Narrative Review

Abstract

Osteoarthritis of the knee is a disorder of the joint that results in changes to the histology and biochemistry of knee joint. Pharmacological and non-pharmacological treatment methods are applied for knee osteoarthritis. Non-pharmacological methods include herbal applications. Application of monosodium iodoacetate (mia) is a frequently used method in studies of knee osteoarthritis. Studies of these models aimed to reduce pain and inflammation in the knee area, prevent apoptosis and slow the development of osteoarthritis. While applying this model, plant and plant-based applications are also analyzed. In our review, plant and plant-based treatments applied in monosodium iodoacetate-induced knee osteoarthritis models are discussed according to plants, administration way of plants, species, mia amount used, solvent, slaughtered days after mia induction and pathways. It is aimed to contribute to the preclinical discussions by compiling the studies.

Keywords

• Osteoarthritis
• Plant
• Treatments
• Monosodium iodoacetate

References

1. 1. Cui A, Li H, Wang D, Zhong J, Chen Y, Lu H. Global, regional prevalence, incidence and risk factors of knee osteoarthritis in population-based studies. EClinicalMedicine. 2020;29.
2. 2. Uthman OA. Global, regional, national incidence, prevalence and years lived with disability for 310 acute and chronic diseases and injuries 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet. 2016;388(10053):1545-602.
3. 3. Loeser RF, Goldring SR, Scanzello CR, Goldring MB. Osteoarthritis: a disease of the joint as an organ. Arthritis and rheumatism. 2012;64(6):1697.
4. 4. Aşkın A, Özkan A, Tosun A, Demirdal ÜS, İsnaç F. Quality of life and functional capacity are adversely affected in osteoarthritis patients with neuropathic pain. The Kaohsiung Journal of Medical Sciences. 2017;33(3):152-8.
5. 5. Vitaloni M, Botto-van Bemden A, Sciortino R, Carné X, Quintero M, Santos-Moreno P, et al. A patients’ view of OA: the Global Osteoarthritis Patient Perception Survey (GOAPPS), a pilot study. BMC musculoskeletal disorders. 2020;21(1):727.
6. 6. Lee D, Ju M-K, Kim H. Commiphora extract mixture ameliorates monosodium iodoacetateinduced osteoarthritis. Nutrients. 2020;12(5):1477.
7. 7. Lim HJ, Park SI, Bak SG, Cheong SH, Lee S, Baek YB, et al. Beneficial effects of Vigna angularis extract in osteoporosis and osteoarthritis. Food Sci Nutr. 2020;8(12):6550-6.
8. 8. Madzuki IN, Lau SF, Abdullah R, Mohd Ishak NI, Mohamed S. Vernonia amygdalina inhibited osteoarthritis development by antiinflammatory and anticollagenase pathways in cartilage explant and osteoarthritis-induced rat model. Phytotherapy Research. 2019;33(7):1784-93.

9. 9. Sa L, Wei X, Huang Q, Cai Y, Lu D, Mei R, et al. Contribution of salidroside to the relieve of symptom and sign in the early acute stage of osteoarthritis in rat model. Journal of Ethnopharmacology. 2020;259:112883.
10. 10. Fusco R, Siracusa R, Peritore AF, Gugliandolo E, Genovese T, D’Amico R, et al. The role of cashew (Anacardium occidentale L.) nuts on an experimental model of painful degenerative joint disease. Antioxidants. 2020;9(6):511.
11. 11. Bethapudi B, Murugan S, Illuri R, Mundkinajeddu D, Velusami CC. Bioactive turmerosaccharides from Curcuma longa Extract (NR-INF-02): Potential ameliorating effect on osteoarthritis pain. Pharmacognosy magazine. 2017;13(Suppl 3):S623.
12. 12. Zhang H, Ding L, Shi X, Mei W, Huang Z, Zhang L, et al. Imperatorin alleviated NLR family pyrin domain-containing 3 inflammasome cascade-induced synovial fibrosis and synovitis in rats with knee osteoarthritis. Bioengineered. 2021;12(2):12954-64.
13. 13. Choi DJ, Choi S-I, Choi B-R, Lee Y-S, Lee DY, Kim GS. Cartilage protective and anti-analgesic effects of ALM16 on monosodium iodoacetate induced osteoarthritis in rats. BMC Complementary and Alternative Medicine. 2019;19(1):325.
14. 14. Chun JM, Lee AY, Moon BC, Choi G, Kim J-S. Effects of Dipsacus asperoides and Phlomis umbrosa Extracts in a Rat Model of Osteoarthritis. Plants. 2021;10(10):2030.
15. 15. Jo HG, Lee GY, Baek CY, Song HS, Lee D. Analgesic and Anti-Inflammatory Effects of Aucklandia lappa Root Extracts on Acetic Acid-Induced Writhing in Mice and Monosodium Iodoacetate-Induced Osteoarthritis in Rats. Plants (Basel). 2020;10(1).
16. 16. Jhun JY, Na HS, Shin JW, Jung KA, Seo HB, Ryu JY, et al. Notoginseng radix and rehmanniae radix preparata extract combination (YH23537) reduces pain and cartilage degeneration in rats with monosodium iodoacetate-induced osteoarthritis. Journal of Medicinal Food. 2018;21(8):745-54.
17. 17. Bahtiar A, Nurazizah M, Roselina T, Tambunan AP, Arsianti A. Ethanolic extracts of babandotan leaves (Ageratum conyzoides L.) prevents inflammation and proteoglycan degradation by inhibiting TNF-α and MMP-9 on osteoarthritis rats induced by monosodium iodoacetate. Asian Pacific journal of tropical medicine. 2017;10(3):270-7.
18. 18. Lee YM, Son E, Kim SH, Kim OS, Kim DS. Anti-inflammatory and anti-osteoarthritis effect of Mollugo pentaphylla extract. Pharm Biol. 2019;57(1):74-81.
19. 19. Hong JM, Shin JK, Kim JY, Jang MJ, Park SK, Lee JH, et al. BST106 Protects against Cartilage Damage by Inhibition of Apoptosis and Enhancement of Autophagy in Osteoarthritic Rats. Biol Pharm Bull. 2018;41(8):1257-68.
20. 20. Tong L, Yu H, Huang X, Shen J, Xiao G, Chen L, et al. Current understanding of osteoarthritis pathogenesis and relevant new approaches. Bone research. 2022;10(1):60.
21. 21. Brumat P, Kunšič O, Novak S, Slokar U, Pšenica J, Topolovec M, et al. The surgical treatment of osteoarthritis. Life. 2022;12(7):982.
22. 22. Kuyinu EL, Narayanan G, Nair LS, Laurencin CT. Animal models of osteoarthritis: classification, update, and measurement of outcomes. Journal of orthopaedic surgery and research. 2016;11(1):19.
23. 23. Kim HL, Lee HJ, Lee D-R, Choi B-K, Yang SH. Herbal composition LI73014F2 alleviates articular cartilage damage and inflammatory response in monosodium iodoacetate-induced osteoarthritis in rats. Molecules. 2020;25(22):5467.
24. 24. Ju L, Hu P, Chen P, Xue X, Li Z, He F, et al. Huoxuezhitong capsule ameliorates MIAinduced osteoarthritis of rats through suppressing PI3K/Akt/NF-κB pathway. Biomedicine & Pharmacotherapy. 2020;129:110471.
25. 25. Shin M-R, Kim H-Y, Choi H-Y, Park KS, Choi HJ, Roh S-S. Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis Through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis. Evidence-based Complementary and Alternative Medicine. 2022;2022(1):3067526.
26. 26. Orhan C, Tuzcu M, Durmus AS, Sahin N, Ozercan IH, Deeh PBD, et al. Protective effect of a novel polyherbal formulation on experimentally induced osteoarthritis in a rat model. Biomedicine & Pharmacotherapy. 2022;151:113052.
27. 27. Aborehab NM, El Bishbishy MH, Refaiy A, Waly NE. A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis. BMC complementary and alternative medicine. 2017;17(1):495.
28. 28. Abdel-Rahman RF, Abd-Elsalam RM, Amer MS, El-Desoky AM, Mohamed SO. Manjarix attenuated pain and joint swelling in a rat model of monosodium iodoacetate-induced osteoarthritis. Food & function. 2020;11(9):7960-72.
29. 29. Balkrishna A, Sinha S, Karumuri S, Srivastava J, Haldar S, Varshney A. Peedanil Gold, Herbo-Mineral Formulation, Moderates Cytokine Levels and Attenuates Pathophysiology in Monosodium Iodoacetate Induced Osteoarthritis in SD Rat Model. Front Pharmacol. 2022;13:883475.
30. 30. Lee YM, Son E, Kim SH, Kim DS. Effect of Alpinia oxyphylla extract in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model. Phytomedicine. 2019;65:153095.
31. 31. Choudhary D, Adhikary S, Ahmad N, Kothari P, Verma A, Trivedi PK, et al. Prevention of articular cartilage degeneration in a rat model of monosodium iodoacetate induced osteoarthritis by oral treatment with Withaferin A. Biomed Pharmacother. 2018;99:151-61.
32. 32. Yimam M, Lee YC, Kim TW, Moore B, Jiao P, Hong M, et al. Analgesic and anti-Inflammatory effect of UP3005, a botanical composition Containing two standardized extracts of Uncaria gambir and Morus alba. Pharmacognosy Res. 2015;7(Suppl 1):S39-46.
33. 33. Kim WK, Chung HJ, Pyee Y, Choi TJ, Park HJ, Hong JY, et al. Effects of intra-articular SHINBARO treatment on monosodium iodoacetate-induced osteoarthritis in rats. Chin Med. 2016;11:17.
34. 34. Kim JK, Park SW, Kang JW, Kim YJ, Lee SY, Shin J, et al. Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats. Evid Based Complement Alternat Med. 2012;2012:730907.
35. 35. Zhang Y, Zeng Y. Curcumin reduces inflammation in knee osteoarthritis rats through blocking TLR4 /MyD88/NF-κB signal pathway. Drug Dev Res. 2019;80(3):353-9.
36. 36. Murugan S, Bethapudi B, Purusothaman D, Chandrasekaran PR, Velusami CC. Antiarthritic effect of polar extract of Curcuma longa on monosodium iodoacetate induced osteoarthritis in rats. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Inflammatory and Anti-Allergy Agents). 2017;16(3):193-202.
37. 37. Wang C, Al-Ani MK, Sha Y, Chi Q, Dong N, Yang L, et al. Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis. Int J Biol Sci. 2019;15(1):229-38.
38. 38. Maresca M, Micheli L, Di Cesare Mannelli L, Tenci B, Innocenti M, Khatib M, et al. Acute effect of Capparis spinosa root extracts on rat articular pain. J Ethnopharmacol. 2016;193:456-65.
39. 39. Chin KY, Wong SK, Japar Sidik FZ, Abdul Hamid J, Abas NH, Mohd Ramli ES, et al. The Effects of Annatto Tocotrienol Supplementation on Cartilage and Subchondral Bone in an Animal Model of Osteoarthritis Induced by Monosodium Iodoacetate. Int J Environ Res Public Health. 2019;16(16).
40. 40. Sa L, Wei X, Huang Q, Cai Y, Lu D, Mei R, et al. Contribution of salidroside to the relieve of symptom and sign in the early acute stage of osteoarthritis in rat model. J Ethnopharmacol. 2020;259:112883.
41. 41. Wan Osman WN, Che Ahmad Tantowi NA, Lau SF, Mohamed S. Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti-inflammatory, anti-oxidative, and antiprotease pathways. J Food Biochem. 2019;43(3):e12755.
42. 42. Wan Osman WN, Lau SF, Mohamed S. Scopoletin-standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study. Phytother Res. 2017;31(12):1954-61.
43. 43. Mohammadifar M, Aarabi MH, Aghighi F, Kazemi M, Vakili Z, Memarzadeh MR, et al. Anti-osteoarthritis potential of peppermint and rosemary essential oils in a nanoemulsion form: behavioral, biochemical, and histopathological evidence. BMC Complement Med Ther. 2021;21(1):57.
44. 44. Hong J-M, Shin J-K, Kim J-Y, Jang M-J, Park S-K, Lee J-H, et al. BST106 protects against cartilage damage by inhibition of apoptosis and enhancement of autophagy in osteoarthritic rats. Biological and Pharmaceutical Bulletin. 2018;41(8):1257-68.
45. 45. Zhang X, Chen R, Liao Z, Zhu Y, Chen Y, Liu J, et al. The Xiaogu San Attenuates Pain and Cartilage Damage in Rats with Monosodium Iodoacetate Induced Osteoarthritis. Comb Chem High Throughput Screen. 2022;25(11):1914-51.
46. 46. Lee YM, Son E, Kim S-H, Kim D-S. Effect of Alpinia oxyphylla extract in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model. Phytomedicine. 2019;65:153095.
47. 47. Park YJ, Cho YR, Oh JS, Ahn EK. Effects of Tribulus terrestris on monosodium iodoacetate‑induced osteoarthritis pain in rats. Mol Med Rep. 2017;16(4):5303-11.
48. 48. Choudhary D, Kothari P, Tripathi AK, Singh S, Adhikary S, Ahmad N, et al. Spinacia oleracea extract attenuates disease progression and sub-chondral bone changes in monosodium iodoacetate-induced osteoarthritis in rats. BMC Complement Altern Med. 2018;18(1):69.
49. 49. Zhang L, Li T, Wang R, Xu J, Zhou L, Yan L, et al. Evaluation of Long-Time Decoction-Detoxicated Hei-Shun-Pian (Processed Aconitum carmichaeli Debeaux Lateral Root With Peel) for Its Acute Toxicity and Therapeutic Effect on Mono-Iodoacetate Induced Osteoarthritis. Front Pharmacol. 2020;11:1053.
50. 50. Woo YJ, Joo YB, Jung YO, Ju JH, Cho ML, Oh HJ, et al. Grape seed proanthocyanidin extract ameliorates monosodium iodoacetate-induced osteoarthritis. Exp Mol Med. 2011;43(10):561-70.
51. 51. Jeong JW, Kim J, Choi EO, Kwon DH, Kong GM, Choi IW, et al. Schisandrae Fructus ethanol extract ameliorates inflammatory responses and articular cartilage damage in monosodium iodoacetate-induced osteoarthritis in rats. Excli j. 2017;16:265-77.
52. 52. Kim SH, Choi HJ, Yang WK, Lee JE, Cho JH, Park IJ, et al. Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats. Evid Based Complement Alternat Med. 2017;2017:1791403.
53. 53. Kumari RR, More AS, Gupta G, Lingaraju MC, Balaganur V, Kumar P, et al. Effect of alcoholic extract of Entada pursaetha DC on monosodium iodoacetate-induced osteoarthritis pain in rats. Indian J Med Res. 2015;141(4):454-62.
54. 54. Lee YM, Son E, Kim S-H, Kim D-S. Antiinflammatory and analgesic effects of Schisandra chinensis leaf extracts and monosodium iodoacetate-induced osteoarthritis in rats and acetic acid-induced writhing in mice. Nutrients. 2022;14(7):1356.
55. 55. Lim HJ, Park SI, Bak SG, Cheong SH, Lee S, Baek YB, et al. Beneficial effects of Vigna angularis extract in osteoporosis and osteoarthritis. Food Science & Nutrition. 2020;8(12):6550-6.
56. 56. Heydari Nasrabadi M, Parsivand M, Mohammadi N, Asghari Moghaddam N. Comparison of Elaeagnus angustifolia L. extract and quercetin on mouse model of knee osteoarthritis. J Ayurveda Integr Med. 2022;13(2):100529.
57. 57. Jeong YJ, Kim I, Cho JH, Park DW, Kwon JE, Jung MW, et al. Anti-osteoarthritic effects of the Litsea japonica fruit in a rat model of osteoarthritis induced by monosodium iodoacetate. PLoS One. 2015;10(8):e0134856.
58. 58. Yamada EF, Olin LC, Pontel CL, da Rosa HS, Folmer V, da Silva MD. Sida tuberculata reduces oxidative stress and pain caused by the knee osteoarthritis. Journal of Ethnopharmacology. 2020;248:112277.
59. 59. Ma P, Yue L, Yang H, Fan Y, Bai J, Li S, et al. Chondroprotective and anti-inflammatory effects of amurensin H by regulating TLR4/Syk/NF-κB signals. J Cell Mol Med. 2020;24(2):1958-68.
60. 60. Wu W, Xu X, Dai Y, Xia L. Therapeutic effect of the saponin fraction from Clematis chinensis Osbeck roots on osteoarthritis induced by monosodium iodoacetate through protecting articular cartilage. Phytother Res. 2010;24(4):538-46.
61. 61. Ju L, Hu P, Chen P, Xue X, Li Z, He F, et al. Huoxuezhitong capsule ameliorates MIAinduced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway. Biomed Pharmacother. 2020;129:110471.
62. 62. Feng D, Zhu X. Preventive Effect of Saussurea lappa Extract on Osteoarthritis in Mice Model through Inhibition of NF-κB Pathway. Dokl Biochem Biophys. 2021;496(1):56-61.
63. 63. Min GY, Park JM, Joo IH, Kim DH. Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-κB signaling pathway. Int J Med Sci. 2021;18(4):861-72.
64. 64. Tantowi NACA, Hussin P, Lau SF, Mohamed S. Mistletoe fig (Ficus deltoidea Jack) leaf extract prevented postmenopausal osteoarthritis by attenuating inflammation and cartilage degradation in rat model. Menopause. 2017;24(9):1071-80.
65. 65. Tantowi NACA, Mohamed S, Lau SF, Hussin P. Comparison of diclofenac with apigeninglycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model. DARU Journal of Pharmaceutical Sciences. 2020;28(2):443-53.
66. 66. Kwon JY, Lee SH, Jhun J, Choi J, Jung K, Cho KH, et al. The combination of probiotic complex, rosavin, and zinc improves pain and cartilage destruction in an osteoarthritis rat model. Journal of medicinal food. 2018;21(4):364-71.
67. 67. Kwon HO, Lee M, Kim O-K, Ha Y, Jun W, Lee J. Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models. Nutrition research and practice. 2016;10(3):265-73.
68. 68. Wang C, Guo L, Tian F, An N, Luo L, Hao R, et al. Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model. Brazilian Journal of Medical and Biological Research. 2017;50(4):e5714.
69. 69. Kim C, Kim JH, Lee S, Lee C, Jang GY, Choi J, et al. Chrysanthemum zawadskii ethanol extract inhibits the replication of alpha-coronavirus and beta-coronavirus. PLoS One. 2025;20(6):e0326225.
70. 70. Kang M, Jung I, Hur J, Kim SH, Lee JH, Kang J-Y, et al. The analgesic and anti-inflammatory effect of WIN-34B, a new herbal formula for osteoarthritis composed of Lonicera japonica Thunb and Anemarrhena asphodeloides BUNGE in vivo. Journal of ethnopharmacology. 2010;131(2):485-96.
71. 71. Wang Q, Zhuang D, Feng W, Ma B, Qin L, Jin L. Fraxetin inhibits interleukin-1β-induced apoptosis, inflammation, and matrix degradation in chondrocytes and protects rat cartilage in vivo. Saudi Pharm J. 2020;28(12):1499-506.
72. 72. Kothari P, Tripathi AK, Girme A, Rai D, Singh R, Sinha S, et al. Caviunin glycoside (CAFG) from Dalbergia sissoo attenuates osteoarthritis by modulating chondrogenic and matrix regulating proteins. Journal of ethnopharmacology. 2022;282:114315.
73. 73. Chun JM, Kim HS, Lee AY, Kim S-H, Kim HK. Anti-inflammatory and antiosteoarthritis effects of Saposhnikovia divaricata ethanol extract: in vitro and in vivo studies. Evidence-Based Complementary and Alternative Medicine. 2016;2016(1):1984238.
74. 74. Park YM, Shin DY, Lee HY, Hwang HM, Kim JG, Kim BS, et al. Pinus densiflora Root Extract Attenuates Osteoarthritis Progression by Inhibiting Inflammation and Cartilage Degradation in Interleukin-1β and Monosodium Iodoacetate-Induced Osteoarthritis Models. Nutrients. 2024;16(22):3882.
75. 75. Jeong J-H, Moon S-J, Jhun J-Y, Yang E-J, Cho M-L, Min J-K. Eupatilin exerts antinociceptive and chondroprotective properties in a rat model of osteoarthritis by downregulating oxidative damage and catabolic activity in chondrocytes. PloS one. 2015;10(6):e0130882.
76. 76. Chun JM, Lee AY, Kim JS, Choi G, Kim S-H. Protective effects of Peucedanum japonicum extract against osteoarthritis in an animal model using a combined systems approach for compound-target prediction. Nutrients. 2018;10(6):754.
77. 77. Lu YC, Jayakumar T, Duann YF, Chou YC, Hsieh CY, Yu SY, et al. Chondroprotective role of sesamol by inhibiting MMPs expression via retaining NF-κB signaling in activated SW1353 cells. J Agric Food Chem. 2011;59(9):4969-78.
78. 78. Jia L, Gong Y, Jiang X, Fan X, Ji Z, Ma T, et al. Ginkgolide C inhibits ROS-mediated activation of NLRP3 inflammasome in chondrocytes to ameliorate osteoarthritis. Journal of Ethnopharmacology. 2024;325:117887.
79. 79. Kuyinu EL, Narayanan G, Nair LS, Laurencin CT. Animal models of osteoarthritis: classification, update, and measurement of outcomes. J Orthop Surg Res. 2016;11:19.
80. 80. Jo H-G, Lee G-Y, Baek CY, Song HS, Lee D. Analgesic and anti-inflammatory effects of aucklandia lappa root extracts on acetic acidinduced writhing in mice and monosodium iodoacetate-induced osteoarthritis in rats. Plants. 2020;10(1):42.
81. 81. Bao Z, Chen M, Li C, Shan Q, Wang Y, Yang W. Monosodium iodoacetate-induced subchondral bone microstructure and inflammatory changes in an animal model of osteoarthritis. Open Life Sci. 2022;17(1):781-93.
82. 82. Wang XD, Kou XX, He DQ, Zeng MM, Meng Z, Bi RY, et al. Progression of cartilage degradation, bone resorption and pain in rat temporomandibular joint osteoarthritis induced by injection of iodoacetate. PLoS One. 2012;7(9):e45036.
83. 83. de Sousa Valente J. The Pharmacology of Pain Associated With the Monoiodoacetate Model of Osteoarthritis. Front Pharmacol. 2019;10:974.