Absorbable and Non-Absorbable Treatment Materials’ Effects on Growth Factors in Traumatic Wound Healing

Abstract

Aim: Comparison of rapidly absorbable monoflament “Polydioxanon”, non-absorbable monoflament “Polypropylene” and slowly absorbable polyflament “Polycaprolactone” wound treatment material sutures’ effects on growth factors in wound healing by an experimental design with biochemical analyses. Methods: Ankara University Ethical Council number 20.14-16-111 permission was taken and 8-12 weeks old, 150-200 gram, 72 male Wistar albino rats were randomly separated into nine groups that each had eight rats. The Sham 7th and 14th, Polypropylene 7th and 14th, Polydioxanon 7th and 14th, Polycaprolactone 7th and 14th groups, besides a Control group were formed. On 7th day of wound formation Sham 7 th, Polypropylene 7 th, Polycaprolactone 7 th, Polydioxanone 7 th groups each containing eight rats tissues’ were sampled (n=32). The Sham 14th, Polypropylene 14th, Polydioxanon 14th, Polycaprolactone 14th groups that each had eight rats (n=32) and control group (n=8) were tissue sampled on 14th day (n=40). “Collagen”, “FGF”, “VEGF”, “EGF” and “KGF” tissue levels were calculated (Ʃn=72). “Suture construction was the stable, flament contructions and absorbability were variable parameters”. Findings: Slowly absorbable “Polycaprolacton” and rapidly absorbable “Polydioxanon” treatment materials “elevated the collagen” formation. “The highest FGF” level which was “responsable for collagen formation” were detected with “Polycaprolacton” and in wound healing vascularization responsible VEGF was observed “the lowest” with “Polypropylene”. Finally,“Polycaprolactone” suture “elevated KGF the most” and “improved skin healingas potent epithelisant”. Conculsion: Absorbable “Polycaprolacton” and “Polydioxanon” both had “the highest collagen” level in tissues which could indicate “effectiveness of soluble treatment materials” in wound healing. “The highest FGF” responsible for “the highest collagen” formation was found with absorbable “Polycaprolacton”. On 14th day absorbable “Polycaprolactone” was observed as “the prevailed epitelisant” among all groups and had “the highest KGF levels” for skin wound healing. “The lowest VEGF levels” that decelerates vascularization delaying wound healing was observed with non-absorbable “Polypropylene”. Key words: Wound Healing, Physical Trauma, Growth Factors, Treatment.

Keywords

• Wound Healing
• Trauma
• Growth Factors
• Treatment Meterials

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Ethical Statement

As the authors we declare that this research is original and that it has never been presented anywhere before besides has never been published in any journal. There is no conflict of interest in this research.

Thanks

We respectfully request your acceptance of this original research for publication. Thank you for your efforts.

Travmatik Yara İyileşmesinde Emilebilen ve Emilemeyen Tedavi Gereçlerinin Büyüme Faktörleri Üzerine Etkisi

Abstract

Amaç: Hızlı emilebilen monoflaman "Polidioksanon", emilemeyen monoflaman "Polipropilen" ve yavaş emilebilen poliflaman "Polikaprolakton" yara tedavi gereçleri olan sutürlerin travmatik yara iyileşmesinde büyüme faktörleri üzerindeki etkilerinin deneysel bir model ve biyokimyasal analizlerle karşılaştırılmasıdır. Yöntem: Ankara Üniversitesi Etik Kurulu 20.14-16-111 numaralı izin alınarak 8-12 haftalık, 150-200 gram ağırlığında, 72 adet erkek Wistar albino sıçan her grupta kör örneklemle rasgele seçilen sekiz sıçan içeren dokuz gruba ayırılarak Sham 7. ve 14. gün, Polipropilen 7 ve 14. gün, Polidioksanon 7. ve 14.gün, Polikaprolakton 7. ve 14.gün ile Kontrol denek grupları oluşturuldu (n=72). Yara oluşumunun 7.gününde her biri sekiz sıçan içeren Sham, Polipropilen 7.gün, Polidioksanon 7.gün, Polikaprolacton 7.gün grubu deneklerinden doku örnekleri alındı (n=32). Herbiri sekiz rat içeren Polipropilen 14.gün, Polikaprolacton 14.gün, Polidioksanon 14.gün ve Sham 14.gün (n=32) ve kontrol grubu (n=8) deneklerinin dokuları ise 14.günde örneklendi (n=40). “Kolajen”, “FGF”, “VEGF”, “EGF” ve “KGF” düzeyleri doku örneklerinde hesaplandı (Ʃn=72). “Sutür yapısı stabil, flament yapıları ve emilebilirlik değişken parametreler”idi. Bulgular: Yavaş emilebilen “Polikaprolakton” ve hızlı emilebilen “Polidioksanon” tedavi materyalleri “Kolajen oluşumunu artırmıştır”. Kolajen oluşumundan sorumlu “en yüksek FGF” düzeyi dokuda emilebilen “Polikaprolakton” ile; yara dokusunda iyileşme için damarlaşmanın artmasından sorumlu olan “VEGF’nin en düşük düzeyi” dokuda emilemeyen “Polipropilen” ile gözlemlenmiştir. Dokuda emilebilir olan “Polikaprolakton” ise araştırmada “KGF'yi en fazla artıran kuvvetli bir epitelizan” olarak saptanmıştır. Sonuç: Emilebilen “Polikaprolakton” ve “Polidioksanon” her ikisi de dokularda “en yüksek Kolajen” oluşturmuştur bu ise yara iyileşmesinde “emilebilen materyallerin etkinliğini” düşündürmektedir. En yüksek Kolajen oluşumundan sorumlu “en yüksek FGF” ise emilebilen “Polikaprolakton”ile bulunmuştur. Emilebilen “Polikaprolakton” araştırmanın 14. gününde tüm gruplar arasında“en yüksek KGF düzeylerini” gösterip cilt yarası için “güçlü bir epitelizan” olmuştur. Damarlanmayı yavaşlatıp iyileşmeyi geciktirebilecek “en düşük VEGF” düzeyini ise “Polipropilen” oluşturduğu gözlenmiştir. Anahtar kelimeler: Yara İyileşmesi, Fiziksel Travma, Büyüme Faktörleri, Tedavi.

Keywords

• Yara iyileşmesi
• Travma
• Büyüme Faktörleri
• Tedavi Materyalleri

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Ethical Statement

Yazarlar olarak bu araştırmanın özgün bir araştırma olduğunu beyan eder; bu araştırmanın daha önce hiçbir yerde sunulmadığını ve hiçbir dergide daha önce yayınlanmamış olduğunu bildiririz. Bu araştırmada çıkar çatışması yoktur.

Thanks

Bu özgün araştırmanın tarafınızca yayınlanmak üzere kabulünü Saygılarımızla arz eder . Verilecek emekler için teşekkür ederiz.

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