Absorbable and Non-Absorbable Treatment Materials’ Effects on Growth Factors in Traumatic Wound Healing

Öz

Aim: Comparison of rapidly absorbable monoflament “Polydioxanon”, non-absorbable monoflament “Polypropylene” and slowly absorbable polyflament “Polycaprolactone” wound treatment material sutures’ effects on growth factors in wound healing by an experimental design with biochemical analyses. Methods: Ankara University Ethical Council number 20.14-16-111 permission was taken and 8-12 weeks old, 150-200 gram, 72 male Wistar albino rats were randomly separated into nine groups that each had eight rats. The Sham 7th and 14th, Polypropylene 7th and 14th, Polydioxanon 7th and 14th, Polycaprolactone 7th and 14th groups, besides a Control group were formed. On 7th day of wound formation Sham 7 th, Polypropylene 7 th, Polycaprolactone 7 th, Polydioxanone 7 th groups each containing eight rats tissues’ were sampled (n=32). The Sham 14th, Polypropylene 14th, Polydioxanon 14th, Polycaprolactone 14th groups that each had eight rats (n=32) and control group (n=8) were tissue sampled on 14th day (n=40). “Collagen”, “FGF”, “VEGF”, “EGF” and “KGF” tissue levels were calculated (Ʃn=72). “Suture construction was the stable, flament contructions and absorbability were variable parameters”. Findings: Slowly absorbable “Polycaprolacton” and rapidly absorbable “Polydioxanon” treatment materials “elevated the collagen” formation. “The highest FGF” level which was “responsable for collagen formation” were detected with “Polycaprolacton” and in wound healing vascularization responsible VEGF was observed “the lowest” with “Polypropylene”. Finally,“Polycaprolactone” suture “elevated KGF the most” and “improved skin healingas potent epithelisant”. Conculsion: Absorbable “Polycaprolacton” and “Polydioxanon” both had “the highest collagen” level in tissues which could indicate “effectiveness of soluble treatment materials” in wound healing. “The highest FGF” responsible for “the highest collagen” formation was found with absorbable “Polycaprolacton”. On 14th day absorbable “Polycaprolactone” was observed as “the prevailed epitelisant” among all groups and had “the highest KGF levels” for skin wound healing. “The lowest VEGF levels” that decelerates vascularization delaying wound healing was observed with non-absorbable “Polypropylene”. Key words: Wound Healing, Physical Trauma, Growth Factors, Treatment.

Anahtar Kelimeler

• Wound Healing
• Trauma
• Growth Factors
• Treatment Meterials

Destekleyen Kurum

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Proje Numarası

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Etik Beyan

As the authors we declare that this research is original and that it has never been presented anywhere before besides has never been published in any journal. There is no conflict of interest in this research.

Teşekkür

We respectfully request your acceptance of this original research for publication. Thank you for your efforts.

Travmatik Yara İyileşmesinde Emilebilen ve Emilemeyen Tedavi Gereçlerinin Büyüme Faktörleri Üzerine Etkisi

Öz

Amaç: Hızlı emilebilen monoflaman "Polidioksanon", emilemeyen monoflaman "Polipropilen" ve yavaş emilebilen poliflaman "Polikaprolakton" yara tedavi gereçleri olan sutürlerin travmatik yara iyileşmesinde büyüme faktörleri üzerindeki etkilerinin deneysel bir model ve biyokimyasal analizlerle karşılaştırılmasıdır. Yöntem: Ankara Üniversitesi Etik Kurulu 20.14-16-111 numaralı izin alınarak 8-12 haftalık, 150-200 gram ağırlığında, 72 adet erkek Wistar albino sıçan her grupta kör örneklemle rasgele seçilen sekiz sıçan içeren dokuz gruba ayırılarak Sham 7. ve 14. gün, Polipropilen 7 ve 14. gün, Polidioksanon 7. ve 14.gün, Polikaprolakton 7. ve 14.gün ile Kontrol denek grupları oluşturuldu (n=72). Yara oluşumunun 7.gününde her biri sekiz sıçan içeren Sham, Polipropilen 7.gün, Polidioksanon 7.gün, Polikaprolacton 7.gün grubu deneklerinden doku örnekleri alındı (n=32). Herbiri sekiz rat içeren Polipropilen 14.gün, Polikaprolacton 14.gün, Polidioksanon 14.gün ve Sham 14.gün (n=32) ve kontrol grubu (n=8) deneklerinin dokuları ise 14.günde örneklendi (n=40). “Kolajen”, “FGF”, “VEGF”, “EGF” ve “KGF” düzeyleri doku örneklerinde hesaplandı (Ʃn=72). “Sutür yapısı stabil, flament yapıları ve emilebilirlik değişken parametreler”idi. Bulgular: Yavaş emilebilen “Polikaprolakton” ve hızlı emilebilen “Polidioksanon” tedavi materyalleri “Kolajen oluşumunu artırmıştır”. Kolajen oluşumundan sorumlu “en yüksek FGF” düzeyi dokuda emilebilen “Polikaprolakton” ile; yara dokusunda iyileşme için damarlaşmanın artmasından sorumlu olan “VEGF’nin en düşük düzeyi” dokuda emilemeyen “Polipropilen” ile gözlemlenmiştir. Dokuda emilebilir olan “Polikaprolakton” ise araştırmada “KGF'yi en fazla artıran kuvvetli bir epitelizan” olarak saptanmıştır. Sonuç: Emilebilen “Polikaprolakton” ve “Polidioksanon” her ikisi de dokularda “en yüksek Kolajen” oluşturmuştur bu ise yara iyileşmesinde “emilebilen materyallerin etkinliğini” düşündürmektedir. En yüksek Kolajen oluşumundan sorumlu “en yüksek FGF” ise emilebilen “Polikaprolakton”ile bulunmuştur. Emilebilen “Polikaprolakton” araştırmanın 14. gününde tüm gruplar arasında“en yüksek KGF düzeylerini” gösterip cilt yarası için “güçlü bir epitelizan” olmuştur. Damarlanmayı yavaşlatıp iyileşmeyi geciktirebilecek “en düşük VEGF” düzeyini ise “Polipropilen” oluşturduğu gözlenmiştir. Anahtar kelimeler: Yara İyileşmesi, Fiziksel Travma, Büyüme Faktörleri, Tedavi.

Anahtar Kelimeler

• Yara iyileşmesi
• Travma
• Büyüme Faktörleri
• Tedavi Materyalleri

Destekleyen Kurum

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Proje Numarası

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Etik Beyan

Yazarlar olarak bu araştırmanın özgün bir araştırma olduğunu beyan eder; bu araştırmanın daha önce hiçbir yerde sunulmadığını ve hiçbir dergide daha önce yayınlanmamış olduğunu bildiririz. Bu araştırmada çıkar çatışması yoktur.

Teşekkür

Bu özgün araştırmanın tarafınızca yayınlanmak üzere kabulünü Saygılarımızla arz eder . Verilecek emekler için teşekkür ederiz.

Kaynakça

1. 1. Wu S, Dong T, Li Y, Sun M, Qi Y, Liu J, Kuss M A, Chen S, Duan B. State of the art review of advanced electrospun nanofiber yarn based textiles for biomedical applications. Appl Mater Today. 2022;27:101473. doi: 10.1016/j.apmt.2022.101473.
2. 2. Cesur O, Tanır TE, Celepli P, Ozarslan F, Hucmenoglu S, Karaibrahimoglu A, Hasirci N. Enhancing esophageal repair with bioactive bilayer mesh containing FGF. Scientefic Reports. 2021;11(1):19203. doi: 10.1038/s41598-021-98840-w.
3. 3. Guo S, Wang P, Sun Y, Cao C, Gao J, Hong S, Li N, Xu R. Transformation of Natural Resin Resina Draconis to 3D Functionalized Fibrous Scaffolds for Efficient Chronic Wound Healing. Adv Healthc Mater. 2024;13(30):e2401105. doi:10.1002/adhm.202401105.
4. 4. Abdelkhalek MA, Abdelaal MM, Abdel Gaber SA. Cold atmospheric plasma followed by in-situ electrospinning of arginine-loaded nanofibers promote infected diabetic wound healing. Int J Pharm. 2025;683:126023. doi:10.1016/j.ijpharm.2025.126023.
5. 5. Benıto-Martinez S, Perez-Kohler B, Rodriguez M, Garcia-Moreno F, Gomez-Gil V, Pascual G, Bellon JM. Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection. Polymers (Basel). 2021;13(14):2371. doi: 10.3390/polym13142371.
6. 6. Sofii I, Widodo I, Gunadi I, Handaya AY. Free omental patch as the promising future for incisional hernia surgery: an experimental study in rat model. Med J Malaysia. 2025;80(2):174-177. 7. Heene S, Thoms S, Kalies S, Wegner N, Peppermüller P, Born N, Walter F, Scheper T, Blume CA. Vascular Network Formation on Macroporous Polydioxanone Scaffolds. Tissue Eng Part A. 2021;27(19-20):1239-1249. doi: 10.1089/ten.TEA.2020.0232.
7. 8. Jayachandran V, Murugan SS, Dalavi PA, Vishalakshi YDG, Seong GH. Alginate based Composite Microspheres: Preparations and Applications for Bone Tissue Engineering. Curr Pharm Des. 2022;28(13):1067-1081. doi:10.2174/1381612828666220518142911.
8. 9. Wu X, Jia Y, Sun X, Wang J. Acceleration of pelvic tissue generation by over expression of basic fibroblast growth factor in stem cells. Connect Tissue Res. 2022;63(3):256-268. doi: 10.1080/03008207.2021.1895130.

9. 10. Lotfi Z, Khakbiz M, Davari N, Bonakdar S, Mohammadi J, Shokrgozar MA, Derhambaksh S. Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing. Artif Organs. 2023;47(8):1267-1284. doi: 10.1111/aor. 145 18.
10. 11. Moyers-Montoya ED, Escobedo-Gonzalez RG, Vargas-Requena CL, Garcia-Casillas PE, Martinez-Perez CA. Epithelial Growth Factor anchored on Polycaprolactone/6-deoxy-6-amino-beta-cyclodextrin Nanofibers: In Vitro In Vivo Evaluation. Polymers (Basel). 2021;13(8):1303. doi:10.3390/polym13081303.
11. 12. Singh R, Roopmani P, Hasan U, Dogra P, Giri J. Airbrushed nanofibers with bioactive core and antibacterial shell for wound healing application. Eur J Pharm Biopharm. 2024;195:114169. doi:10.1016/j.ejpb.2023.12.009. 13. Wu Z, Xu L, Xu X, Hou J, Li W, Lou G, Xu Y, Chen Q, Cui F. Loureirin hydrogel promotes healing of radionuclide-contaminated wounds by regulating angiogenesis and immune cells. Biomaterials Science. 2024;12(22):5789-5802.
12. 14. Zhang X, Hao R, Tong J, Hu H, Du J, Gong B, Tian F, Lu Y, Xue J. A radially aligned nanofiber scaffold with engineered guidance gradients for directed cell migration and accelerated wound healing. Biomaterials. 2025;327:123797. doi: 10.1039/D4BM00813H.
13. 15. Pasqua M, Barbuto M, Calligaris M, Di Gesu R, Pagano D, Busa R, Gruttaduaria S, Scilabra SD, D’Amore A, Francipane MG. Engineering a Human-Sized Common Bile Duct Prototype with Regenerative Potential: In Vitro Evaluation of Mechanics, Function, Degradation and Immune Modulation. Adv Healthc Mater. 2025;14(21):e2501660. doi: 10.1002/adhm.202501660.
14. 16. Devin PK, Farahpour MR, Tabatabaei ZG. Multicomponent biopolymer hydrogels based on polycaprolactone with the combination of nano silver and linalool for the healing of infectious wounds. Int Immunopharmacol. 2025:148:114075. doi:10.1016/j.intimp.2025.1140 75.
15. 17. Lee S, Moon HW, Lee SJ, Cho JC. Development and Characterization of PEGylated Poly D, L-Lactic Acid Nanoparticles for Skin Rejuvenation. Nanomaterials(Basel). 2025;15(6):470. doi: 10.3390/nano15060470.
16. 18. Ha YI, Kim JH, Park ES. Histological and molecular biological analysis on the reaction of absorbable thread; Polydioxanone and Polycaprolactone in rat model. J Cosmet Dermatol. 2022;21(7):2774-2782. doi: 10.1111/jocd.14587.
17. 19. Cho SW, Shin BH, Heo CY, Shim JH. Efficacy study of the new polycaprolactone thread compared with other commercialized threads in a murine model. J Cosmet Dermatol. 2021;20(9):2743-2749. doi: 10.1111/jocd.13883.
18. 20. Nikhil A, Gugjoo MB, Das A, Ahmad SM, Kumar A. 3D-Printed-Cryogel-Impregnated Functionalized Scaffold Augments Bone Regeneration in Critical Tibia Fracture in Goat. Adv Healthc Mater. 2024;13(32): e2402619. doi: 10.1002/adhm.202402619.
19. 21. Chen YP, Lo TS, Lin YT, Chien YH, Lu CJ, Liu SJ. Fabrication of Drug Eluting Polycaprolactone/polylactic-co-glycolicacid Prolapse Mats Using Solution Extrusion 3D Printing and Coaxial Electrospinning Techniques. Polymers (Basel). 2021;13(14):2295. doi:10.3390/polym13142295