Farklı Matrislerde Pioglitazon ve Metforminin Yüksek Performanslı Sıvı Kromatografi Yöntemleri ile Nicel Analizine Yönelik Güncel Yaklaşımlar

Abstract

Diyabet, sıklıkla karşılaşılan ve yüksek ölüm oranına sahip bir hastalıktır. Pankreasın beta hücrelerinin tahrip olması sonucu pankreastan insülin salgılanamaması veya insüline karşı direnç gelişmesiyle insülin görevini yapamaz hale gelir ve sonuç olarak hastalık yüksek glikoz seviyesi ile karekterize olur. Vakaların büyük çoğunluğunu insüline bağımlı olmayan (tip 2) diyabet oluşturur. Diyabet çoğu zaman asemptomatik olsa da klinik belirtileri, poliüri, polidipsi, polifaji ve açıklanamayan kilo kaybı şeklinde sıralanabilir. Komplikasyonları arasında, ülserler, ampütasyonlar, retinopati, böbrek yetmezliği ve kalp hastalıkları sıralanabilir. Diyabet hastalığının tedavisinde hedef glikoz seviyesini azaltmak veya korumaktır. Bu amaçla hastaya ilk olarak, egzersiz ve diyet değişikliği ile yaşam tarzı değişiklikleri önerilir. Glikoz düzeyi, bu tavsiyeler ile düzenlenmiyorsa hastaya oral antidiyabetik ajan kullanması önerilir. Bu çalışmada, diyabet tedavisinde kullanılan oral antidiyabetik ajanlardan metformin ve pioglitazonun, analitik kimyada sıklıkla kullanılan bir kromatografik yöntem olan HPLC ile yapılan miktar analizlerine yer verilmiştir.

Keywords

• Metformin
• Pioglitazon
• HPLC
• Analitik Kimya

Current Approaches to Quantitative Analysis of Pioglitazone and Metformin in Different Matrices by High-Performance Liquid Chromatography Methods

Abstract

Diabetes is a common disease with a high mortality rate. Damage to the pancreatic beta cells results in the pancreas failing to secrete insulin or developing insulin resistance, rendering insulin unable to function effectively. Consequently, the disease is characterized by high glucose levels. Non-insulin-dependent (type 2) diabetes accounts for most cases. While diabetes is often asymptomatic, clinical symptoms include polyuria, polydipsia, polyphagia, and unexplained weight loss. Complications include ulcers, amputations, retinopathy, renal failure, and heart disease. The goal of treating diabetes is to reduce or maintain glucose levels. To this end, patients are initially advised to make lifestyle changes, including exercise and dietary changes. If glucose levels are not controlled with these recommendations, oral antidiabetic agents are recommended. This study includes quantitative analyses of metformin and pioglitazone, oral antidiabetic agents used in the treatment of diabetes, using HPLC, a chromatographic method frequently used in analytical chemistry.

Keywords

• Metformin
• Pioglitazone
• HPLC
• Analytical Chemistry

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